Determination of thymol in halothane anaesthetic preparations by high-performance liquid chromatography

Thompson, Richard; Carlson, Marvin

doi:10.1016/0731-7085(89)80055-x

Cited by 17 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This value was similar to the EC 50 value for blocking Ca 2+ and Na + currents [7], [8] and for inducing Ca 2+ release from internal stores [4], [5]. Thymol is used as a stabilizing agent for liquid halothane used in general anesthesia [24]. Thymol is used as a stabilizing agent for liquid halothane used in general anesthesia [24].…”

Section: Discussionsupporting

confidence: 64%

Stimulatory Actions of Thymol, a Natural Product, on Ca²⁺-Activated K⁺Current in Pituitary GH₃Cells

Huang¹,

Wu²,

Shen³

2005

Planta med

View full text Add to dashboard Cite

Drugs that influence the opening of potassium (K(+)) channels and as a consequence cause hyperpolarization of cell membrane possess clinical potential. The large conductance Ca(2+)-activated K(+) (BK) channel is highly selective for K(+). Activation of this channel is Ca(2+)- and voltage-dependent. We have investigated the effects of thymol, a natural product, on ion currents in pituitary GH(3) cells. The patch-clamp technique was used to investigate the effect of thymol (100 microM) in these cells. Thymol reversibly stimulated the Ca(2+)-activated K(+) current with an EC (50) value of 75 microM. In a cell-attached configuration, application of thymol to the bath increased the activity of BK channels. BAPTA (1 mM) attenuated thymol-stimulated channel activity. In an experiment using the inside-out configuration, thymol exposed to the intracellular face of excised patches did not modify the single-channel conductance of these channels whereas it enhanced the channel activity. Neither menthol (100 microM) nor zingerone (100 microM) had an effect on BK-channel activity while AAPH (100 microM) suppressed it significantly. The stimulatory actions of thymol on Ca(2+)-activated K(+) currents may be associated with the underlying cellular mechanisms through which it affects neuronal or neuroendocrine functions.

show abstract

Section: Discussionsupporting

confidence: 64%

Stimulatory Actions of Thymol, a Natural Product, on Ca²⁺-Activated K⁺Current in Pituitary GH₃Cells

Huang¹,

Wu²,

Shen³

2005

Planta med

View full text Add to dashboard Cite

show abstract

“…Although the concentration of thymol is relatively low (0.01 %) in the original halothane-thymol mixture [17], its concentration may dramatically increase due to accumulation of thymol in the vaporizer. Indeed, halothane was shown to block Ca and K currents in cardiac [18-20] and skeletal [21] muscle membranes.…”

Section: Discussionmentioning

confidence: 99%

Effect of thymol on kinetic properties of Ca and K currents in rat skeletal muscle

et al. 2003

View full text Add to dashboard Cite

Background: Thymol is widely used as a general antiseptic and antioxidant compound in the medical practice and industry, and also as a stabilizer to several therapeutic agents, including halothane. Thus intoxication with thymol may occur in case of ingestion or improper anesthesia. In the present study, therefore, concentration-dependent effects of thymol (30-600 micro-grams) were studied on calcium and potassium currents in enzymatically isolated rat skeletal muscle fibers using the double vaseline gap voltage clamp technique.

show abstract

“…CX and TH were acetone‐extracted, in duplicate, from individual 1‐cm catheter segments, and lyophilised extracts were subsequently reconstituted in HPLC buffer consisting of methanol:water (75:25). Individual samples were tested in a reversed‐phase HPLC system using a C18 column with a modified flow rate of 2.5 mL/min isocratically as described previously [9]. HPLC analysis indicated that a 1‐cm segment of antimicrobial‐impregnated urinary catheter contained an average of 1.4 mg of CX and 4.2 mg of TH.…”

Section: In‐vitro Zones Of Inhibition Against Uropathogens (Mm)mentioning

confidence: 99%

In-vitro activity and in-vivo efficacy of catheters impregnated with chloroxylenol and thymol against uropathogens

Mansouri

Darouiche

2008

Clinical Microbiology and Infection

View full text Add to dashboard Cite

Determination of thymol in halothane anaesthetic preparations by high-performance liquid chromatography

Cited by 17 publications

References 9 publications

Stimulatory Actions of Thymol, a Natural Product, on Ca²⁺-Activated K⁺Current in Pituitary GH₃Cells

Stimulatory Actions of Thymol, a Natural Product, on Ca²⁺-Activated K⁺Current in Pituitary GH₃Cells

Effect of thymol on kinetic properties of Ca and K currents in rat skeletal muscle

In-vitro activity and in-vivo efficacy of catheters impregnated with chloroxylenol and thymol against uropathogens

Contact Info

Product

Resources

About

Determination of thymol in halothane anaesthetic preparations by high-performance liquid chromatography

Cited by 17 publications

References 9 publications

Stimulatory Actions of Thymol, a Natural Product, on Ca2+-Activated K+Current in Pituitary GH3Cells

Stimulatory Actions of Thymol, a Natural Product, on Ca2+-Activated K+Current in Pituitary GH3Cells

Effect of thymol on kinetic properties of Ca and K currents in rat skeletal muscle

In-vitro activity and in-vivo efficacy of catheters impregnated with chloroxylenol and thymol against uropathogens

Contact Info

Product

Resources

About

Stimulatory Actions of Thymol, a Natural Product, on Ca²⁺-Activated K⁺Current in Pituitary GH₃Cells

Stimulatory Actions of Thymol, a Natural Product, on Ca²⁺-Activated K⁺Current in Pituitary GH₃Cells