Determination of tigecycline in turkey plasma by LC-MS/MS: validation and application in a pharmacokinetic study

Abstract

Tigecycline (TIG), a novel glycylcycline antibiotic, plays an important role in the management of complicated skin and intra-abdominal infections. The available data lack any description of a method for determination of TIG in avian plasma. In our study, a selective, accurate and reversed-phase high performance liquid chromatography-tandem mass spectrometry method was developed for the determination of TIG in turkey plasma. Sample preparation was based on protein precipitation and liquid-liquid extraction using 1,2-dichloroethane. Chromatographic separation of TIG and minocycline (internal standard, IS) was achieved on an Atlantis T3 column (150 mm x 3.0 mm, 3.0 μm) using gradient elution. The selected reaction monitoring transitions were performed at 293.60 m/z → 257.10 m/z for TIG and 458.00 m/z → 441.20 m/z for IS. The developed method was validated in terms of specificity, selectivity, linearity, lowest limit of quantification, limit of detection, precision, accuracy, matrix effect, carry-over effect, extraction recovery and stability. All parameters of the method submitted to validation met the acceptance criteria. The assay was linear over the concentration range of 0.01-100 μg/ml. This validated method was successfully applied to a TIG pharmacokinetic study in turkey after intravenous and oral administration at a dose of 10 mg/kg at various time-points.

Pharmacokinetics of tigecycline in turkeys following different routes of administration

“…The plasma concentration of TIG was determined using the sample preparation and the validated liquid chromatography (LC) coupled with a mass spectrometry (LC/MS‐MS) analytical method described previously by Jasiecka‐Mikołajczyk and Jaroszewski (). The analytical method was performed in a reversed‐phase LC system Alliance 2695 coupled with a tandem mass spectrometer (MS/MS) Quattro Micro API MS (Waters, Milford, MA, USA).…”

Comparison of adsorption of selected antibiotics on the filters in continuous renal replacement therapy circuits: in vitro studies

“…The tigecycline concentrations in collected samples were determined using fully validated LC with tandem mass spectrometry detection analytical method described previously by Jasiecka-Mikołajczyk and Jaroszewski with minor modifications, which were shorter column (50 mm instead of 150 mm) and tigecycline-d9 as the internal standard (instead of minocycline) [13]. The calibration curve ranged from 0.1 to 100 μg/mL and the limit of detection was 0.01 μg/mL.…”

Comparative pharmacokinetics of chlortetracycline, tetracycline, minocycline, and tigecycline in broiler chickens

“…Fully validated analytical methods were used in the Alliance 2695 HPLC system coupled with the Quattro micro API MS tandem mass spectrometer (Waters, Milford) according to the protocols described by Jasiecka-Mikołajczyk and Jaroszewski (2017) and Ziółkowski et al. (2016) .…”

“…Plasma samples for the determination of TIG and MIN were prepared based on the analytical method of Jasiecka-Mikołajczyk and Jaroszewski (2017) with minor modifications (TIG-d9 was the IS, and a shorter column was used). Moreover, for CTC and TET analysis the sample preparation method described by Ziółkowski et al.…”