Determination of Total Apigenin in Herbs by Micellar Electrokinetic Chromatography with UV Detection

Głowacki, Rafał; Furmaniak, Paulina; Kubalczyk, Paweł; Borowczyk, Kamila

doi:10.1155/2016/3827832

Cited by 13 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The content of apigenin in parsley depends on the place where it was bought. Such conclusions were drawn by Głowacki et al [24]. In our study, the lower content of apigenin was determined (0.69 mg/g DW).…”

Section: Resultssupporting

confidence: 88%

Mechanism of Action and Interactions between Thyroid Peroxidase and Lipoxygenase Inhibitors Derived from Plant Sources

2019

View full text Add to dashboard Cite

This study focused on the effect of kaempferol, catechin, apigenin, sinapinic acid, and extracts from plants (i.e., parsley, cumin, mustard, green tea, and green coffee) on thyroid peroxidase (TPO) and lipoxygenase (LOX) activity, antiradical potential, as well as the result of interactions among them. Catechin, sinapinic acid, and kaempferol acted as a competitive TPO inhibitors, while apigenin demonstrated an uncompetitive mode of inhibitory action. Ethanol extracts from all plants acted as competitive TPO inhibitors, while, after in vitro digestion, TPO activation was found especially in the case of mustard (24%) and cumin (19.85%). Most importantly, TPO activators acted synergistically. The TPO effectors acted as LOX inhibitors. The most effective were potentially bioaccessible compounds from green tea and green coffee (IC50 = 29.73 mg DW/mL and 30.43 mg DW/mL, respectively). The highest free radical scavenging ability was determined for catechin and sinapinic acid (IC50 = 78.37 µg/mL and 84.33 µg/mL, respectively) and potentially bioaccessible compounds from mustard (0.42 mg DW/mL) and green coffee (0.87 mg DW/mL). Green coffee, green tea, cumin, and mustard contain potentially bioaccessible TPO activators that also act as effective LOX inhibitors, which indicate their potentially health-promoting effects for people suffering from Hashimoto’s disease.

show abstract

Section: Resultssupporting

confidence: 88%

Mechanism of Action and Interactions between Thyroid Peroxidase and Lipoxygenase Inhibitors Derived from Plant Sources

2019

View full text Add to dashboard Cite

show abstract

“…In the last 10 years, several studies have been carried out to develop analytical methods for these metabolites. Maher et al [ 125 ] developed a method for the detection of luteolin and apigenin in thyme and parsley, using an electrolyte solution of borax and methanol, while Głowacki et al [ 126 ] used MEKC for apigenin analysis. In the same way, methods for the analysis of bioactive curcuminoids from turmeric herbal products have been developed using a non-aqueous background electrolyte [ 127 ], borate [ 128 ], and 20 mM Triton X-100, 20 mM SDS, 30% ( v / v ) methanol in 10 mM borax solution at pH 10.0 [ 129 ].…”

Section: Methods For Detecting Spice and Herb Frauds And Adulterationsmentioning

confidence: 99%

Spice and Herb Frauds: Types, Incidence, and Detection: The State of the Art

Velázquez,

Rodríguez,

Hernández

et al. 2023

Foods

View full text Add to dashboard Cite

There is a necessity to protect the quality and authenticity of herbs and spices because of the increase in the fraud and adulteration incidence during the last 30 years. There are several aspects that make herbs and spices quite vulnerable to fraud and adulteration, including their positive and desirable sensorial and health-related properties, the form in which they are sold, which is mostly powdered, and their economic relevance around the world, even in developing countries. For these reasons, sensitive, rapid, and reliable techniques are needed to verify the authenticity of these agri-food products and implement effective adulteration prevention measures. This review highlights why spices and herbs are highly valued ingredients, their economic importance, and the official quality schemes to protect their quality and authenticity. In addition to this, the type of frauds that can take place with spices and herbs have been disclosed, and the fraud incidence and an overview of scientific articles related to fraud and adulteration based on the Rapid Alert System Feed and Food (RASFF) and the Web of Science databases, respectively, during the last 30 years, is carried out here. Next, the methods used to detect adulterants in spices and herbs are reviewed, with DNA-based techniques and mainly spectroscopy and image analysis methods being the most recommended. Finally, the available adulteration prevention measurements for spices and herbs are presented, and future perspectives are also discussed.

show abstract

“…Borate buffer was often employed in separation of flavonoids due to its ability to create anionic complexes with compounds possessing neighboring hydroxyl groups such as luteolin or [50] 2003 Trojanowicz quercetin. [79,80] The optimization of separation was achieved by change of the buffer pH, parameters of organic solvents and surfactant (SDS) and finally detection parameters in UV or LIF detectors. [48][49][50] The migration of flavonoids and their resolution is mostly influenced by buffer characteristics (pH and concentration), surfactant (concentration) and type of organic solvents (MeOH or ACN).…”

Section: Yellow Dyes-analysis With Electromigration Techniquesmentioning

confidence: 99%

Analysis of Natural Dyes from Historical Objects by High Performance Liquid Chromatography and Electromigration Techniques

Zasada-Kłodzińska

Basiul

Buszewski

et al. 2020

Critical Reviews in Analytical Chemistry

View full text Add to dashboard Cite

Based on material published between 1989 and 2018 in this paper high performance liquid chromatography and electromigration techniques used in studies of natural dyes that can be found in historical objects are rewieved. Different aspects of analysis have been discussed: the stationary and mobile phase, the choice of sample solvent, methods of extraction and detection, including sensitivity parameters, such as LOD and/or LOQ. The discussed dyes have been divided into three categories (a) red antraquinone dyes along with dyes extracted from bark and tree juices, (b) yellow flavonoid dyes and saffron and (c) blue indigoid dyes. The main markers (chromophores or auxochromes) typical for each dye source were presented which allows to identify specific species of source plants and animals. The first part of the study involved the analysis of most critical findings when it comes to HPLC or represented an significant analytical approach. The second part of the study is focused on different aspects of electromigration techniques application in analysis of natural dyes with special attention paid on such parameters as the running buffer/mobile phase composition and sample solvent. Detection methods along with LOD and LOQ comparison in HPLC and electromigration techniques were also discussed. Methods of sample preparation, such as hydrolysis and extraction, used in HPLC and electromigration techniques were also briefly discussed.

show abstract

Determination of Total Apigenin in Herbs by Micellar Electrokinetic Chromatography with UV Detection

Cited by 13 publications

References 31 publications

Mechanism of Action and Interactions between Thyroid Peroxidase and Lipoxygenase Inhibitors Derived from Plant Sources

Mechanism of Action and Interactions between Thyroid Peroxidase and Lipoxygenase Inhibitors Derived from Plant Sources

Spice and Herb Frauds: Types, Incidence, and Detection: The State of the Art

Analysis of Natural Dyes from Historical Objects by High Performance Liquid Chromatography and Electromigration Techniques

Contact Info

Product

Resources

About