Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

Кучеренко, І. С.; Солдаткін, О. О.; Lagarde, Florence; Jaffrézic‐Renault, Nicole; Dzyadevych, S. V.

doi:10.1016/j.talanta.2015.06.079

Cited by 22 publications

(9 citation statements)

References 13 publications

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“…Serum levels of creatine kinase (CK) enzyme activities were measured using the CK activity assay kits (CK-20; Sigma Chemical Co.) as described for CK activity in serum by Kucherenko et al (2015). The se-rum levels of malondialdehyde (MDA) were measured using the thiobarbituric acid assay following Khoubnasabjafari (2015) method.…”

Section: Biochemical Assessmentmentioning

confidence: 99%

Histological Studies of the Heart and Biochemical Changes Due to the Perinatal Consumption of Hibiscus sabdariffa (Flavonoid-rich Extract) to Feed-restricted Rats on Offspring

et al. 2023

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Background: Experiments using animal models have indicated that maternal diet during the perinatal period can drastically impact the life-long susceptibility of offspring to chronic disease. Objectives: This study aims to determine the impact of perinatal consumption of Hibiscus sabdariffa to feed restriction on offspring heart development. Methods: In this study, we used 25 pregnant rats. They were grouped as follows: group 1 (normal control), group 2 (70% feed restriction), group 3 (70% feed restriction and 5mg/kg body weight of H. sabdariffa administration), group 4 (70% feed restriction, and 10mg/kg body weight of H. sabdariffa administration), and group 5 (70% feed restriction and 20mg/kg body weight of H. sabdariffa administration). Dams were only allowed to nurse 8 pups. Pups were weaned and observed daily for puberty onset. Blood samples were collected to determine serum levels of creatine kinase, hydrogen peroxide, and malondialdehyde. The hearts were excised, weighed, and prepared for histological examination. Results: The result showed a significant increase in absolute and relative weights of the heart with alterations in the histology of the heart. There was a significant change in serum levels of creatine kinase and malondialdehyde. Conclusion: Maternal consumption of H. sabdariffa during pregnancy and lactation may increase the development of cardiovascular diseases in offspring during postnatal growth.

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Section: Biochemical Assessmentmentioning

confidence: 99%

Histological Studies of the Heart and Biochemical Changes Due to the Perinatal Consumption of Hibiscus sabdariffa (Flavonoid-rich Extract) to Feed-restricted Rats on Offspring

et al. 2023

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“…Late identification and treatment cause organ damage and lead to death, and rapid identification of MI is mandatory to save the patient's life 4 . Various blood‐based biomarkers, including creatine kinase, myoglobin, MB isozymes, troponins C, I, and T, have been found to be suitable for diagnosing MI 5,6 . Among them, troponin was proven to be an efficient biomarker, and cardiac troponin I (cTnI) is a unique protein in the muscle cells of the heart that has a high correlation with MI and serves as an efficient biomarker to detect MI 7–9 .…”

Section: Introductionmentioning

confidence: 99%

Cardiovascular biomarker troponin I biosensor: Aptamer‐gold‐antibody hybrid on a metal oxide surface

Hu¹,

Gopinath

Ismail

et al. 2022

Biotech and App Biochem

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Myocardial infarction (MI) is highly related to cardiac arrest leading to death and organ damage. Radiological techniques and electrocardiography have been used as preliminary tests to diagnose MI; however, these techniques are not sensitive enough for early‐stage detection. A blood biomarker‐based diagnosis is an immediate solution, and due to the high correlation of troponin with MI, it has been considered to be a gold‐standard biomarker. In the present research, the cardiac biomarker troponin I (cTnI) was detected on an interdigitated electrode sensor with various surface interfaces. To detect cTnI, a capture aptamer‐conjugated gold nanoparticle probe and detection antibody probe were utilized and compared through an alternating sandwich pattern. The surface metal oxide morphology of the developed sensor was proven by microscopic assessments. The limit of detection with the aptamer‐gold‐cTnI‐antibody sandwich pattern was 100 aM, while it was 1 fM with antibody‐gold‐cTnI‐aptamer, representing 10‐fold differences. Further, the high performance of the sensor was confirmed by selective cTnI determination in serum, exhibiting superior nonfouling. These methods of determination provide options for generating novel assays for diagnosing MI.

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“…For example, luciferase‐based bioluminescence is considered the gold standard for in situ ATP detection even at sub‐nanomolar concentrations [20]. There are techniques that can be used to measure physiologically‐relevant ATP concentrations including: spectrophotometry [21], liquid chromatography [22–25], fluorescence [26, 27], chemiluminescence [28–30], bioluminescence [31, 32] and electrochemical methods using potentiometric [33, 34] and amperometric biosensors [35–49]. Of these, only the electrochemical biosensors would be useful for transient changes in ATP concentration in vitro or in vivo near the sites of release.…”

Section: Introductionmentioning

confidence: 99%

“…Amperometric ATP biosensors have been prepared and employed with a common design using hexokinase (Hex) and glucose oxidase (GOx) incorporated within a polymer matrix [35, 36, 38, 40, 43–45]. This dual enzyme biosensor reports changes in the ATP concentration indirectly via reduction of the glucose concentration in solution as the substrate reacts with ATP at Hex to form glucose 3‐phosphate [35, 36, 38, 40, 43–45]. This reduces the H 2 O 2 concentration at the electrode, which is detected as a decrease in the oxidation current at +0.85 V vs. Ag/AgCl.…”

Section: Introductionmentioning

confidence: 99%

An Electrochemical ATP Biosensor with Enzymes Entrapped within a PEDOT Film

et al. 2020

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This paper reports on the electrochemical behavior of an ATP biosensor that utilizes glucose oxidase (GOx) and hexokinase (Hex) immobilized within the electroactive polymer, polyethylenedioxythiophene (PEDOT). This biosensor design detects ATP indirectly at 0.85 V vs. Ag/AgCl based on the oxidation current for enzymatically generated H2O2, and at −0.20 V; a potential at which improved analyte selectivity is achieved. The detection figures of merit at both detection potentials are a response time of 15±1 s, an experimental detection limit of 10.0±0.2 μmol L−1 (S/N=3), and a sensitivity in the range of 100–500 mA M−1cm−2.

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Determination of total creatine kinase activity in blood serum using an amperometric biosensor based on glucose oxidase and hexokinase

Cited by 22 publications

References 13 publications

Histological Studies of the Heart and Biochemical Changes Due to the Perinatal Consumption of Hibiscus sabdariffa (Flavonoid-rich Extract) to Feed-restricted Rats on Offspring

Histological Studies of the Heart and Biochemical Changes Due to the Perinatal Consumption of Hibiscus sabdariffa (Flavonoid-rich Extract) to Feed-restricted Rats on Offspring

Cardiovascular biomarker troponin I biosensor: Aptamer‐gold‐antibody hybrid on a metal oxide surface

An Electrochemical ATP Biosensor with Enzymes Entrapped within a PEDOT Film

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