Determination of total cysteamine in human serum by a high-performance liquid chromatography with fluorescence detection

Stachowicz, M.; Lehmann, Blandine; Tibi, Annick; Prognon, P.; Daurat, Véronique; Pradeau, D.

doi:10.1016/s0731-7085(97)00248-3

Cited by 41 publications

(35 citation statements)

References 20 publications

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“…The active surface areas of the modified electrodes are estimated according to the slope of the I p versus ν 1/2 plot for a known concentration of K 2 Fe(CN) 6 , based on the Randles-Sevcik equation,…”

Section: Electrochemistr Y Of 34-dihydroxycinnamic Acid (34-dhca)mentioning

confidence: 99%

“…where I pa refers to the anodic peak current, n the electron transfer number, A the surface area of the electrode, D R the diffusion coefficient, C 0 the concentration of K 2 Fe(CN) 6 and ν is the scan rate. relation, the microscopic areas were calculated.…”

Section: Electrochemistr Y Of 34-dihydroxycinnamic Acid (34-dhca)mentioning

confidence: 99%

“…5 With this goal in mind, a rapid but specific and sufficiently sensitive analytical method was required for total cysteamine determination in biological and pharmaceutical samples. Numerous methods have been reported for the determination of CA in pharmaceutical and biological samples including chromatography, [6][7][8] electrophoresis, 9 gas chromatography with flame photometric detection, 10 ion exchange chromatography, 11 and electrochemical methods [12][13][14] using modified electrodes. Due to problems in chromatographic methods such as selecting a suitable column or a mobile phase with liquid chromatographic methods, long response time, expensive instruments, complicated procedure, and low detection capability [15][16][17] , electrochemical techniques for determination of CA was used in this work.…”

Section: Introductionmentioning

confidence: 99%

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Electrocatalytic determination of cysteamine using multiwall carbon nanotube paste electrode in the presence of 3,4-dihydroxycinnamic acid as a homogeneous mediator

Keyvanfard¹,

Sami²,

Karimi-Maleh³

et al. 2013

J. Braz. Chem. Soc.

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A eletrooxidação da cisteamina (CA) foi estudada por eletrodos de pasta de nanotubos de carbono modificados com ácido 3,4-diidroxicinâmico (3,4-DHCA) usando voltametria cíclica, cronoamperometria e voltametria de varredura linear. Usando o eletrodo modificado, a cinética de eletrooxidação da CA foi consideravelmente intensificada através da redução do potencial anódico por meio de uma reação catalítica. O mecanismo de comportamento eletroquímico da CA na superfície do eletrodo modificado foi analisado por vários métodos eletroquímicos, na presença de mediador. O eletrodo modificado preparado apresentou respostas voltamétricas com alta sensibilidade para CA, o que o torna muito adequado para a detecção de CA em quantidades de traço. Um intervalo dinâmico linear de 0,25-400 µmol L −1 para a CA foi obtida em solução tamponada com pH 7,0. O limite de detecção foi de 0,09 µmol L −1 .The electrooxidation of cysteamine (CA) was studied by modified carbon nanotubes paste electrode in the presence of 3,4-dihydroxycinnamic acid (3,4-DHCA) using cyclic voltammetry, chronoamperometry and linear sweep voltammetry. Using the modified electrode, the kinetics of CA electrooxidation was considerably enhanced by lowering the anodic over-potential through a catalytic fashion. The mechanism of CA electrochemical behavior at the modified electrode surface was analyzed by various electrochemical methods in the presence of mediator. The prepared modified electrode showed voltammetric responses with high sensitivity for CA, making it very suitable for the detection of CA at trace levels. A linear dynamic range of 0.25-400 µmol L −1 for CA was obtained in buffered solutions with pH 7.0. The limit of detection was 0.09 µmol L −1 . Keywords: cysteamine, electrocatalysis, voltammetry, multiwall carbon nanotubes, 3,4-dihydroxycinnamic acid IntroductionCysteamine is an important thiol drug for the treatment of cystinosis.1 The deficiency of a cystine carrier in the lysosomal membrane leads to cystine accumulation within the lysosomes, ultimately crystallizing in vital organs such as the liver, kidney, spleen, intestines, and cornea.2,3 A number of long term clinical trials have shown that cysteamine administration (as cysteamine hydrochloride) stabilizes renal function, delays glomerular deterioration and improves linear growth. 4 Cysteamine and its disulfide, cystamine, have been shown to be neuroprotective in a number of cell culture and animal models.5 With this goal in mind, a rapid but specific and sufficiently sensitive analytical method was required for total cysteamine determination in biological and pharmaceutical samples. Numerous methods have been reported for the determination of CA in pharmaceutical and biological samples including chromatography, 6-8 electrophoresis, 9 gas chromatography with flame photometric detection, 10 ion exchange chromatography, 11 and electrochemical methods 12-14 using modified electrodes. Due to problems in chromatographic methods such as selecting a suitable column or a mobile phase with liquid chrom...

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Section: Electrochemistr Y Of 34-dihydroxycinnamic Acid (34-dhca)mentioning

confidence: 99%

Section: Electrochemistr Y Of 34-dihydroxycinnamic Acid (34-dhca)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrocatalytic determination of cysteamine using multiwall carbon nanotube paste electrode in the presence of 3,4-dihydroxycinnamic acid as a homogeneous mediator

Keyvanfard¹,

Sami²,

Karimi-Maleh³

et al. 2013

J. Braz. Chem. Soc.

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show abstract

“…Several methods have been reported for the determination of CA in different samples including chromatography [5][6][7], electrophoresis [8], gas chromatography with flame photometric detection [9], ion exchange chromatography [10], and electrochemical methods [10,12] using modified electrodes. However, chromatographic methods still have to cope with certain limitations such as selecting a suitable column or a mobile phase while liquid chromatographic methods are limited by finding a suitable reactant for post-column reactions (in order to increase sensitivity).…”

Section: Introductionmentioning

confidence: 99%

A Voltammetric Sensor Based on Modified Multiwall Carbon Nanotubes for Cysteamine Determination in the Presence of Tryptophan Using p‐Aminophenol as a Mediator

Ensafi

Karimi-Maleh

2010

Electroanalysis

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Determination of cysteamine and tryptophan is described by electrochemical methods using p-aminophenol-multiwall carbon nanotubes paste electrode. Cysteamine and tryptophan in mixture can each be measured independently from each other with a potential difference of 600 mV. The results showed that the electrocatalytic currents increased linearly with cysteamine and tryptophan concentrations over the ranges 0.5-300 mmol L À1 and 10.0-650 mmol L À1 , respectively. The detection limits for cysteamine and tryptophan are found to be 0.14 and 5.9 mmol L À1, respectively. The proposed method is successfully employed for the determination of cysteamine in both capsule and urine samples.

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“…Because of CASH's important role in clinical settings and its potential future applications as an antioxidant, it has become necessary to develop analytical methods for detecting CASH in biological samples. Various derivatizing reagents and procedures have been described in the literature for CASH analysis [7][8][9]. These procedures, however, are either complex or time-consuming, involving incubation of samples in the dark, deproteinization, coupled enzyme reactions and pre-treatment of the biological samples.…”

Section: Introductionmentioning

confidence: 99%

Method for determination of total cysteamine in human plasma by high performance capillary electrophoresis with acetonitrile stacking

Kubalczyk

Bald

2008

Electrophoresis

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An analytical procedure enabling routine analysis of human plasma for total cysteamine (CASH) has been developed and validated. The method includes reduction of CASH disulfides to thiol with tri-n-butylphosphine, derivatization of the thiol with 2-chloro-1-methylquinolinium tetrafluoroborate, separation of CASH 2-S-quinolinium derivate from those of plasma endo- and exogenous thiol derivatives by capillary zone electrophoresis based on acetonitrile stacking and quantitation with the use of ultraviolet detection. A large volume of sample is injected to achieve analyte concentration directly on the capillary, according to the transient pseudo-isotachophoresis principle, before the separation step takes place. Method performance characteristics, for example recovery, calibration, precision, limit of detection and limit of quantitation are presented. The procedure was applied to analysis of plasma samples donated by apparently healthy volunteers spiked with known amounts of cystamine standard solution.

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Determination of total cysteamine in human serum by a high-performance liquid chromatography with fluorescence detection

Cited by 41 publications

References 20 publications

Electrocatalytic determination of cysteamine using multiwall carbon nanotube paste electrode in the presence of 3,4-dihydroxycinnamic acid as a homogeneous mediator

Electrocatalytic determination of cysteamine using multiwall carbon nanotube paste electrode in the presence of 3,4-dihydroxycinnamic acid as a homogeneous mediator

A Voltammetric Sensor Based on Modified Multiwall Carbon Nanotubes for Cysteamine Determination in the Presence of Tryptophan Using p‐Aminophenol as a Mediator

Method for determination of total cysteamine in human plasma by high performance capillary electrophoresis with acetonitrile stacking

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