1994
DOI: 10.1093/jaoac/77.3.687
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Determination of Total Dietary Fiber in Foods and Products with Little or No Starch, Nonenzymatic-Gravimetric Method: Collaborative Study

Abstract: A collaborative study was conducted to validate a nonenzymatic-gravimetric method for the determination of total dietary fiber (TDF) of samples containing little or no starch such as most fruits, and vegetables and many purified polysaccharides. This simple procedure involves suspension of freeze-dried, ground samples in deionized water and incubation at 37°C for 90 min, followed by pre-cipitation with 4 volumes of 95% ethanol. The weight of the dilute alcohol-insoluble residues after correcting for crude prot… Show more

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Cited by 35 publications
(8 citation statements)
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“…The ash content was determined by heating 2 g of the dried sample in a silica dish at 600°C for 6 h (AOAC, 2005). Total dietary fibre (TDF) was estimated by the nonenzymatic-gravimetric method (Li & Cardozo, 1994). To determine the TDF, duplicate 500-mg ground samples were taken in separate 250-mL beakers.…”
Section: Proximate Compositionmentioning
confidence: 99%
“…The ash content was determined by heating 2 g of the dried sample in a silica dish at 600°C for 6 h (AOAC, 2005). Total dietary fibre (TDF) was estimated by the nonenzymatic-gravimetric method (Li & Cardozo, 1994). To determine the TDF, duplicate 500-mg ground samples were taken in separate 250-mL beakers.…”
Section: Proximate Compositionmentioning
confidence: 99%
“…A fibra alimentar total foi determinada pelo método gravimétrico não-enzimático (Li & Cardozo, 1994).…”
Section: Caracterização Químicaunclassified
“…With a "gold standard" method in place, nutritional research and analytical chemists began to add improvements or alternative approaches but all having the same objective and results. Lee [40], Mongeau [41], Li [42], Theander [43] and their co-workers developed, validated and carried out successful collaborative studies which were adopted by the AOAC. Noteworthy is the method of Lee et al who substituted MES-TRIS buffer for the phosphate buffer in the original AOAC dietary fiber determination.…”
Section: Introductionmentioning
confidence: 99%