Determination of total mercury in biological tissues by flow injection cold vapour generation atomic absorption spectrometry following tetramethylammonium hydroxide digestion

Tao, Guanhong; Willie, Scott

doi:10.1039/a802000k

Cited by 69 publications

(45 citation statements)

References 26 publications

(39 reference statements)

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“…Among all the species, methylmercury (MeHg) is the organomercury compound most commonly found in fish and is recognized as a major environmental pollutant and health hazard for humans because of its easy penetration through biological membranes, efficient bioaccumulation, high stability, and long-term elimination from tissues [1]. Therefore, the exposure to MeHg supposes a risk for human health because of its teratogenic, immunotoxic, and, most importantly, neurotoxic effects [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Mercury–Selenium Species Ratio in Representative Fish Samples and Their Bioaccessibility by an In Vitro Digestion Method

Cabañero

Madrid

Cámara

2007

Biol Trace Elem Res

117

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The potential toxicity of mercury (Hg) content in fish has been widely evaluated by the scientific community, with Methylmercury (MeHg) being the only legislated species (1 mg kg-1, maximum concentration allowed in predatory fish). On the other hand, selenium (Se) is recognized to decrease its toxicity when both elements are simultaneously administrated. In the present paper, the total content of Se and Hg and their species in fish of high consumption, such as tuna, swordfish, and sardine, have been evaluated. The percentage of MeHg is higher than 90% of total Hg content. The results show that, for all of them, the Se/Hg ratio is significantly higher than one, being the maximum ratio for sardine. As only studying the bioaccessible fraction the extent of a toxic effect caused by an element can be predicted, the bioaccessibility of both analytes through an in vitro digestion method has been carried out. The results show that MeHg in all fishes is very low bioaccessible in both gastric and intestinal digestion. Because the MeHg bioaccessible fraction might be correlated to the Se content, the potential toxicity cannot be only related to the total Hg content but also to Se/Hg ratio.

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Section: Introductionmentioning

confidence: 99%

Mercury–Selenium Species Ratio in Representative Fish Samples and Their Bioaccessibility by an In Vitro Digestion Method

Cabañero

Madrid

Cámara

2007

Biol Trace Elem Res

117

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“…In order to convert all Hg in the solution to Hg 2+ , KMnO 4 was added to the five solutions earlier mentioned, before the reaction with SnCl 2 or with NaBH 4 [19]. The effect of increasing amount of KMnO 4 on the intensity signals for the solutions is shown in Fig.…”

Section: Mercury Vapor Generationmentioning

confidence: 99%

Determination of As and Hg in acetic acid extract by vapor generation coupled to atomic spectrometry for solid waste classification

Maranhão

Silva

Andrade

et al. 2013

Microchemical Journal

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Methods for the determination of As and Hg by vapor generation atomic spectrometry in acetic acid leachates are proposed. The waste classification involves several tests, including the toxicity evaluation by which the solid waste is leached with acetic acid solutions according to procedures specified by Norms as for example USEPA, toxicity characteristic leaching procedure (TCLP). Some elements, such as As and Hg, are determined in the leachate, and if the concentration is higher than the limits of the Norms the waste is classified as hazardous. In this study, two wastes, a retorted shale and a catalyst from hydrothermal treatment, were submitted to the procedures. The reagents concentrations used to generate arsine for further determination by hydride generation atomic absorption spectrometry (HG AAS) were optimized: HCl (5% v/v), KI (3% m/v) and NaBH 4 (1.0% m/v containing 0.1% m/v NaOH). The previous addition of KI to reduce As(V) to As(III) was necessary to obtain accurate results. Mercury was determined by two techniques, cold vapor atomic absorption spectrometry (CV AAS), using NaBH 4 as reducing agent, and cold vapor atomic fluorescence spectrometry (CV AFS), using SnCl 2 as reducing agent. The reagents concentrations were also optimized, for AAS: HCl (0.5% v/v), KMnO 4 (0.25% m/v), hydroxylamine hydrochloride (0.02% m/v) and NaBH 4 (0.5% m/v containing 0.1% m/v NaOH); and for AFS: HCl (2% v/v), KMnO 4 (0.04% m/v), hydroxylamine hydrochloride (0.02% m/v) and SnCl 2 (3% m/v). Addition of KMnO 4 was used for an efficient vapor generation in the presence of acetic acid. The detection limit (3 s, n = 10) for Hg using CV AFS was about two orders of magnitude lower than for CV AAS. All three proposed methods can be used for waste classification, considering that the limits of detection were in the order of ng L − 1 , much lower than the concentration limits of the Norm EPA, 5 mg L − 1 for As and 0.2 mg L − 1 for Hg. Accuracy for As was demonstrated by comparison with the results obtained by ICP-MS, and for Hg it was demonstrated by the recovery test. The results by CV AAS were below the detection limit. The analytes concentrations in the acetic acid leachates from both residues were below the concentration limits of the Norms, demonstrating that they are non-dangerous concerning As and Hg.

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“…Copper is widespread in the environment where its determination is necessary because it is known to be toxic at higher concentrations and causes dyslexia, hypoglycemia, gastrointestinal problems, and Wilson's disease [1,2]. A number of methods such as atomic absorption spectrometry (AAS), cold vapour AAS or flame atomic absorption spectrometry-electrothermal atomization (AAS-ETA) [3][4][5][6], gravimetriy [6], chromatography [7], and photometry [6,[8][9][10][11][12] have been used for its determination. These methods provide accurate analysis but generally require sample pretreatment and expertise with good infrastructure backup and are therefore, not very convenient for routine analysis of large number of environmental and biological samples.…”

Section: Introductionmentioning

confidence: 99%

Copper(II) ion selective electrode based on a newly synthesized Schiff-base chelate

Jain

Singh

Jain

et al. 2007

Transition Met Chem

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A new Cu (II) ion-selective electrode has been fabricated in poly (vinyl chloride) matrix based on a recently synthesized Schiff-base chelate. The addition of sodium tetraphenylborate (NaTPB) and various plasticizers viz. TBP, TEHP, DOS, and CN have been found to substantially improve the performance of the electrode. The membrane of various compositions of the ionophore (I) were investigated and it was found that the best performance was obtained with the membrane having (I): PVC: NaTPB: CN in the ratio 4: 140: 3: 80 (mg). The electrode exhibits a Nernstian response over a wide concentration range (1.9 9 10 -6 -1.0 9 10 -1 M) with 30.0 mV/decade of concentration between pH 3.0 and 7.5. The response time of the electrode is about 12 s and it can be used over a period of 3 months without any divergence in potential. The potentiometric selectivity coefficient values as determined by fixed interference method indicate excellent selectivity for Cu 2+ ions over interfering cations. The electrode has also been used successfully in partially nonaqueous media having a 25% (v/v) methanol, ethanol or acetone content without showing any considerable change in the value of slope or working concentration range. The electrode has been used in the potentiometric titration of Cu 2+ with EDTA.

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Determination of total mercury in biological tissues by flow injection cold vapour generation atomic absorption spectrometry following tetramethylammonium hydroxide digestion

Cited by 69 publications

References 26 publications

Mercury–Selenium Species Ratio in Representative Fish Samples and Their Bioaccessibility by an In Vitro Digestion Method

Mercury–Selenium Species Ratio in Representative Fish Samples and Their Bioaccessibility by an In Vitro Digestion Method

Determination of As and Hg in acetic acid extract by vapor generation coupled to atomic spectrometry for solid waste classification

Copper(II) ion selective electrode based on a newly synthesized Schiff-base chelate

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