Determination of Total Sennosides and Sennosides A, B, and A1 in Senna Leaflets, Pods, and Tablets by Two-Dimensional qNMR

Abstract

In the present work, a two-dimensional qNMR method for the determination of sennosides was established. Using band-selective HSQC and the cross correlations of the characteristic 10–10’ bonds, we quantified the total amount of the value-determining dianthranoids in five minutes, thus, rendering the method not only fast, but also specific and stability indicating. The validation of the method revealed excellent accuracy (recovery rates of 98.5 to 103%), precision (RSD values of 3.1%), and repeatability (2.2%) and demonstrated the potential of 2D qNMR in the quality control of medicinal plants. In a second method, the use of 2D qNMR for the single analysis of sennosides A, B, and A1 was evaluated with acceptable measurement times (31 min), accuracy (93.8%), and repeatability (5.4% and 5.6%) for the two major purgatives sennoside A and B. However, the precision for sennoside B and A1 was not satisfactory, mainly due to the low resolution of the HSQC signals of the two compounds.

Simultaneous rapid detection of glycyrrhizin and sennoside A in Daiokanzoto samples by lateral flow immunoassay

“…These encompass semipreparative reversed-phase HPLC with a photodiode array, 13 ultrahigh-performance liquid chromatography-tandem mass spectrometry, 14 reversed-phase high-performance thin-layer chromatography, 15 HPTLC, 16 and two-dimensional quantitative nuclear magnetic resonance. 17 A few of these methods are distinct modifications or advancements of HPLC. [18][19][20] Despite their capabilities, these techniques present certain limitations.…”

“…Although HPLC remains a primary method, various advanced analytical techniques have been proposed. These encompass semipreparative reversed‐phase HPLC with a photodiode array, 13 ultrahigh‐performance liquid chromatography–tandem mass spectrometry, 14 reversed‐phase high‐performance thin‐layer chromatography, 15 HPTLC, 16 and two‐dimensional quantitative nuclear magnetic resonance 17 . A few of these methods are distinct modifications or advancements of HPLC 18–20 .…”