Determination of toxigenic potential of &lt;i&gt;Fusarium&lt;/i&gt; species occurring on sorghum and maize grains produced in Karnataka, India by using Thin Layer Chromatography

Sreenivasa, M. Y.; Diwakar, BT; Raj, A. P. Charith; Dass, Regina Sharmila; Naidu, K. Akhilender; Janardhana, G. R.

doi:10.3126/ijls.v6i1.5883

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…F. verticillioides are widely distributed in crops and vegetables such as maize, , rice, sugar cane, wheat, banana, asparagus, and sorghum causing stalk and root rots. In addition to causing serious economic losses, this pathogen produces a group of mycotoxins that threaten human and animal health .…”

Section: Resultsmentioning

confidence: 99%

Discovery of Novel Spiropiperidinyl-α-methylene-γ-butyrolactones as Antifungal and Antitoxin Agents Targeting Oxysterol Binding Protein

Yuan,

Yang,

Gao

et al. 2024

J. Agric. Food Chem.

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Corn ear rot and fumonisin caused by Fusarium verticillioides pose a serious threat to food security. To find more highly active fungicidal and antitoxic candidates with structure diversity based on naturally occurring lead xanthatin, a series of novel spiropiperidinyl-α-methylene-γ-butyrolactones were rationally designed and synthesized. The in vitro bioassay results indicated that compound 7c showed broad-spectrum in vitro activity with EC 50 values falling from 3.51 to 24.10 μg/mL against Rhizoctonia solani and Alternaria solani, which was more active than the positive controls xanthatin and oxathiapiprolin. In addition, compound 7c also showed good antitoxic efficacy against fumonisin with a 48% inhibition rate even at a concentration of 20 μg/mL. Fluorescence quenching and the molecular docking validated both 7c and oxathiapiprolin targeting at FvoshC. RNA sequencing analysis discovered that FUM gene cluster and protein processing in endoplasmic reticulum were downregulated. Our studies have discovered spiropiperidinyl-α-methylene-γ-butyrolactone as a novel FvoshC target-based scaffold for fungicide lead with antitoxin activity.

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Section: Resultsmentioning

confidence: 99%

Discovery of Novel Spiropiperidinyl-α-methylene-γ-butyrolactones as Antifungal and Antitoxin Agents Targeting Oxysterol Binding Protein

Yuan,

Yang,

Gao

et al. 2024

J. Agric. Food Chem.

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“…Fumonisins are found in maize or maize-produced products [ 13 ]. Consumption of F. verticilliodes mycotoxin leads to health risks in vital organs (lungs, kidneys, skin), cancer, and others [ 14 , 15 ]. F. solani and F. proliferatum were isolated from date palms from various regions, one of which was Saudi Arabia [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

The Antifungal Properties of Tamarix aphylla Extract against Some Plant Pathogenic Fungi

Al-Otibi¹,

Moria²,

Alharbi³

et al. 2023

Microorganisms

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Tamarix aphylla is a Saudi herb, which possesses antimicrobial properties and potentially introduces a solution to the subsequent dilemma caused by agrochemicals and antifungal misuse. The current study aimed to assess the fungicidal properties of water and ethanolic extracts of T. aphylla leaves against Macrophomina phaseolina, Curvularia spicifera, and Fusarium spp. The chemical composition of T. aphylla was evaluated by gas chromatography/mass spectrometry technique (GC–MS) and Fourier-transform infrared spectroscopy (FTIR). The antifungal assay assessed the fungal growth inhibition using the poisoned food technique. Scanning and transmission electron microscopy (SEM and TEM) were used to evaluate the structural changes induced in the fungal species post-treatment by T. aphylla. FTIR and GC–MS analysis revealed that T. aphylla extracts were rich in aromatic and volatile compounds, such as Benzeneselenol, Gibberellic acid, and Triaziquone, which proved multiple antifungal properties. The results showed significant inhibition in the growth of all species (p < 0.05) except for F. moniliforme, where the water extract induced the highest mycelial growth inhibition at the dose of 30%. The highest inhibition was for M. phaseolina treated with the water extract (36.25 ± 1.06 mm, p < 0.001) and C. spicifera, treated with the ethanolic extract (27.25 ± 1.77 mm, p < 0.05), as compared to the untreated control and the positive control of Ridomol. SEM and TEM revealed some ultrastructural changes within the fungal growth of treated M. phaseolina, which included the thickening and mild rupture of mycelia. Those findings suggested the robust antifungal properties of T. aphylla against some filamentous fungi. The phenolic composition illustrated the potential fungicidal properties of T. aphylla. Additional studies are required to focus on more antimicrobial properties of T. aphylla against other species, particularly those that might benefit the medical field.

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“…Studies have shown that consumption of mycotoxin-contaminated cereals can affect the lungs, liver and kidneys in animals and cause wounds, skin lesions, and even lead to cancer in humans [ 3 , 4 ]. In horses, being fed with naturally contaminated corn, corn screenings, and corn-based feeds or intravenous injection of fumonisin leads to leukoencephalomalacia (LEM) [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Current Perspectives of Biocontrol Agents for Management of Fusarium verticillioides and Its Fumonisin in Cereals—A Review

Deepa

Achar

Sreenivasa

2021

JoF

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Fusarium verticillioides is the most predominant fungal phytopathogen of cereals and it is posing great concern from a global perspective. The fungus is mainly associated with maize, rice, sorghum, wheat, sugarcane, banana, and asparagus and causes cob, stalk, ear, root, crown, top, and foot rot. F. verticillioides produces fumonisins as the major secondary metabolite along with trace levels of beauvericin, fusaric acid, fusarin C, gibberiliformin, and moniliformin. Being a potential carcinogen, fumonisins continue to receive major attention as they are common contaminants in cereals and its processed food products. The importance of elimination of F. verticillioides growth and its associated fumonisin from cereals cannot be overemphasized considering the significant health hazards associated with its consumption. Physical and chemical approaches have been shown to reduce fumonisin B1 concentrations among feeds and food products but have proved to be ineffective during the production process. Hence, biological control methods using microorganisms, plant extracts, antioxidants, essential oils, phenolic compounds, and other advanced technologies such as growing disease-resistant crops by applying genetic engineering, have become an effective alternative for managing F. verticillioides and its toxin. The different methods, challenges, and concerns regarding the biocontrol of F. verticillioides and production of fumonisin B1 have been addressed in the present review.

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Determination of toxigenic potential of <i>Fusarium</i> species occurring on sorghum and maize grains produced in Karnataka, India by using Thin Layer Chromatography

Cited by 6 publications

References 24 publications

Discovery of Novel Spiropiperidinyl-α-methylene-γ-butyrolactones as Antifungal and Antitoxin Agents Targeting Oxysterol Binding Protein

Discovery of Novel Spiropiperidinyl-α-methylene-γ-butyrolactones as Antifungal and Antitoxin Agents Targeting Oxysterol Binding Protein

The Antifungal Properties of Tamarix aphylla Extract against Some Plant Pathogenic Fungi

Current Perspectives of Biocontrol Agents for Management of Fusarium verticillioides and Its Fumonisin in Cereals—A Review

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