Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative “Shape-Fitting” Peak Identification/Quantification Method

Hamase, Kenji; Ikeda, Tatsuhiko; Ishii, Chiharu; Ishigo, Shoto; Masuyama, Kei; Akita, Takeyuki; Furusho, Aogu; Takahashi, Miho; Ide, Tomomi; Mita, Masashi

doi:10.15583/jpchrom.2018.019

Cited by 23 publications

(14 citation statements)

References 29 publications

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“…Target peaks were quantified by scaling the standard peak shape [20] . In this method, the shape of a peak was used for identification of the substrate, whereas the magnitude of the intensity was used for quantification.…”

Section: Methodsmentioning

confidence: 99%

“…Target peaks were quantified by scaling the standard peak shape. 20 In this method, the shape of a peak was used for identification of the substrate, whereas the magnitude of the intensity was used for quantification. Prior to the quantification, peak sections of various concentrations of standard serine enantiomers (D-serine, 199-08822; L-serine, 634-25661; Fujifilm Wako Chemical Corporation, Osaka, Japan) were obtained as standard shapes for calibration.…”

Section: D-serine Quantificationmentioning

confidence: 99%

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Measurement of glomerular filtration rate using endogenous d-serine clearance in living kidney transplant donors and recipients

et al. 2022

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Background Endogenous molecules that provide an unbiased and a precise evaluation of kidney function are still necessary. We explored the potential of clearance of D-serine, a rare enantiomer of serine and a biomarker of kidney function, as a measure of glomerular filtration rate (GFR).Methods This was a cross-sectional observational study of 200 living kidney transplant donors and recipients enrolled between July 2019 and December 2020 in a single Japanese center, for whom GFR was measured by clearance of inulin (C-in). Clearance of D-serine (C-DSer) was calculated based on blood and urine levels of D-serine, as measured by two-dimensional high-performance liquid chromatography. Analytical performance was assessed by calculating biases. Utilizing data from 129 participants, we developed equations for C-in based on C-DSer and C-cre using a linear regression model, and the performance was validated in 68 participants.Findings The means of C-in and C-DSer were 66.7 and 55.7 mL/min/1.73 m 2 of body surface area, respectively, in the entire cohort. C-DSer underestimated C-in with a proportional bias of 22.0% (95% confidence interval, 14.2 −29.8%) and a constant bias of -1.24 (-5.78−3.31), whereas the proportional bias was minor to that of C-cre (34.6% [31.1−38.2%] and 2.47 (-1.18−6.13) for proportional and constant bias, respectively). Combination of C-DSer and Ccre measured C-in with an equation of 0.391 £ C-DSer + 0.418 £ C-cre + 3.852, which reduced the proportional bias (6.5% [-0.2−13.1%] and -4.30 [-8.87−0.28] for proportional and constant bias, respectively). In the validation dataset, this equation performed well with median absolute residual of 3.5 [2.3−4.8], and high ratio of agreement (ratios of 30% and 15% different from C-in [P 30 and P 15 ] of 98.5 [91.4−100] and 89.7 [80.0−95.2], respectively).Interpretation The smaller proportional bias compared to that of C-cre is an advantage of C-DSer as a measure of Cin. Combinational measurement of D-serine and creatinine, two endogenous molecules, has the potential to serve as a measure of GFR with precision and minor biases and can support important clinical decisions.

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Section: Methodsmentioning

confidence: 99%

Section: D-serine Quantificationmentioning

confidence: 99%

Measurement of glomerular filtration rate using endogenous d-serine clearance in living kidney transplant donors and recipients

et al. 2022

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“…The mobile phases are the mixed solution of MeOH–MeCN containing formic acid, and the fluorescence detection of the NBD-amino acids was carried out at 530 nm with excitation at 470 nm using two photomultiplier tubes. Target peaks were quantified by scaling the standard peak shapes [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

Intra-body dynamics of d-serine reflects the origin of kidney diseases

et al. 2021

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Introduction d-Serine, present only in trace amounts in humans, is now recognized as a biomarker of chronic kidney disease (CKD). CKD is heterogeneous in its original kidney diseases, whose diagnoses require kidney biopsy. In this study, we examined whether the intra-body dynamics of d-serine, indexed by its blood and urinary levels, reflects the origin of kidney diseases. Methods Patients with six kinds of kidney disease undergoing kidney biopsy were enrolled in a single center. Levels of d- and l-serine were measured using two-dimensional high-performance liquid chromatography. The associations between the origin of kidney diseases and the intra-body dynamics of d-serine were examined using multivariate cluster analyses. Results Unlike the non-CKD profile, patients with CKD showed broadly-distributed profiles of intra-body dynamics of d-serine. The plasma level of d-serine plays a key role in the detection of kidney diseases, whereas a combination of plasma and urinary levels of d-serine distinguished the origin of CKD, especially lupus nephritis. Conclusion Intra-body dynamics of d-serine have the potential to predict the origin of kidney diseases. Monitoring of d-serine may guide specific treatments for the origin of kidney diseases.

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“…The enantiomers of serine were quantified using the 2D-HPLC platform, as previously described 16,17 , with the shape-fitting algorithm 18 . Briefly, the NBDderivatives of the amino acids were separated from numerous intrinsic substances using a reversed-phase column (Singularity RP column, 1.0 mm i.d.…”

Section: Determination Of Amino Acid Enantiomers By 2d-hplcmentioning

confidence: 99%

d-Serine Mediates Cellular Proliferation for Kidney Remodeling

et al. 2021

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Background: D-Serine, a long-term undetected enantiomer of serine, is a biomarker that reflects kidney function and disease activity. The physiological functions of D-serine have been unclear. Methods: Dynamics of D-serine was assessed by measuring D-serine in human samples of living kidney donors using two-dimensional high-performance liquid chromatography, and by auto-radiographic studies in mice. Effects of D-serine on kidney were examined by gene expression profiling and metabolic studies using unilateral nephrectomy mice, and genetically modified cells. Results: Unilateral nephrectomy in human living kidney donors decreases urinary excretion and thus increases the blood level of D-serine. D-Serine is quickly and dominantly distributed to the kidney upon injection in mice, suggesting that the kidney is a main target organ. Treatment of D-serine at low dose promotes the enlargement of remnant kidney in mouse model. Mechanistically, D-serine activates the cell cycle for tissue remodeling through an mTOR-related pathway. Conclusions: D-Serine is a physiological molecule that promotes kidney remodeling. Besides its function as a biomarker, D-serine has a physiological activity that influences kidney function.

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Determination of Trace Amounts of Chiral Amino Acids in Complicated Biological Samples Using Two-Dimensional High-Performance Liquid Chromatography with an Innovative “Shape-Fitting” Peak Identification/Quantification Method

Cited by 23 publications

References 29 publications

Measurement of glomerular filtration rate using endogenous d-serine clearance in living kidney transplant donors and recipients

Measurement of glomerular filtration rate using endogenous d-serine clearance in living kidney transplant donors and recipients

Intra-body dynamics of d-serine reflects the origin of kidney diseases

d-Serine Mediates Cellular Proliferation for Kidney Remodeling

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