Determination of trans-resveratrol in human plasma by high-performance liquid chromatography

Zhu, Zhigang; Klironomos, Gia; Vachereau, André; Neirinck, Len; Goodman, D. W.

doi:10.1016/s0378-4347(98)00586-6

Cited by 31 publications

(16 citation statements)

References 8 publications

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“…Samples (0.5 ml) of cell culture media were treated for 2 h at 37°C with 200 U of ␤-glucuronidase in 0.5 ml of 0.2 M acetate buffer; control samples were incubated with buffer alone. Each sample was then acidified to pH 4.5, received carbamazepine as internal standard (Zhu et al, 1999), and was passed through a C18 Sep-Pak cartridge (Waters, Milford, MA) preconditioned with methanol and acetate buffer. After washing with acetate buffer, resveratrol and/or its metabolites were eluted with 2 ml of methanol.…”

Section: Methodsmentioning

confidence: 99%

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Lançon¹,

Hanet²,

Jannin³

et al. 2007

Drug Metabolism and Disposition

108

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ABSTRACT:trans-Resveratrol is a polyphenol present in several plant species. Its chemopreventive properties against several diseases have been largely documented. To validate a model for the study of the factors influencing its biological fate at the hepatic level, the metabolism and the efflux of resveratrol were studied in the human hepatoblastoma cell line, HepG2. Comparative high-performance liquid chromatography analysis of cell culture media before and after deconjugation showed that resveratrol was rapidly conjugated; at the concentration of 10 M, it was entirely metabolized at 8 h of incubation. Two main resveratrol metabolites, monosulfate and disulfate, were identified by atmospheric pressure chemical

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Section: Methodsmentioning

confidence: 99%

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Lançon¹,

Hanet²,

Jannin³

et al. 2007

Drug Metabolism and Disposition

108

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“…Part of the development and refinement of the analytical assay included the validation of a gradient elution HPLC assay for plasma and urine. Several methods have been published for the analysis of resveratrol and some of its metabolites [21,[24][25][26] This method is an improvement on the UV-HPLC method developed by Zhu et al, in 1999 [21] as it allows resolution of the several human phase II metabolites of resveratrol, by direct means rather than by deconjugation, (many of which are very polar and difficult to resolve in gradient modifications of the previous method) in addition to trans-resveratrol and uses a mobile phase that can be directly transferred to LC-MSMS for identification of these metabolites. Wang et al, in 2005 [26] published an HPLC-MSMS method for identification of major metabolites in rat urine, this method identified several conjugated metabolites as well as dihydroresveratrol and its monosulfate conjugate that were found in humans by Walle et al, in 2004 [27].…”

Section: Introductionmentioning

confidence: 99%

Quantitation of trans-resveratrol and detection of its metabolites in human plasma and urine by high performance liquid chromatography

Boocock¹,

Patel²,

Faust³

et al. 2007

Journal of Chromatography B

180

134

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We describe a reversed-phase HPLC method that uses gradient elution and UV detection (325 nm) to determine levels of resveratrol and identify 6 major conjugated metabolites in the plasma and urine of human volunteers after administration of a single oral dose of 1 g. Waters Atlantis C 18 3μ served as the stationary phase. The gradient was formed using ammonium acetate and methanol, containing 2% propan-2-ol. Detection is linear between 5 ng/mL and 500 ng/mL in plasma (5-1000 ng/mL in urine). The coefficient of variation for intra-and inter-day variation is <10%. The average recovery of resveratrol from plasma and urine is 58 ± 3%. The data presented in this report demonstrate a rapid, sensitive and accurate method for the analysis of resveratrol and its metabolites in human plasma and urine for pharmacokinetic studies.

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“…t-RES levels were determined in plasma by high pressure liquid chromatography according to the method of Zhu et al (1999) with some modifications in chromatographic conditions [10]. Prior to application to the analytical column, 500 l at plasma was precipated with 250 l of 0.5 N sodium hydroxide.…”

Section: Resveratrol Extraction and Quantitationmentioning

confidence: 99%

HPLC Analysis of Trans-Resveratrol in Human Plasma After Red Wine Consumption

Corre¹,

Léger-Enreille²,

Chalabi³

et al. 2008

TOCBMJ

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Abstract:Trans-resveratrol (t-RES), a phenolic compound produced by several plants and present in wine, has been reported to be a potential chemopreventive agent for cardiovascular, cancer and neurodegenerative pathologies. Thus, understanding the plasma level in vivo of trans-resveratrol is the prerequisite to evaluate its potential health impact. Bioavailability studies mainly in animals or in humans using the pure compound at very high doses were performed. The objective of this present study was to detected trans-resveratrol in human plasma from two subjects who consumed 600 mL of red wine over 40 min. Plasma analyses were performed by HPLC and obtained results indicated the absence of t-RES in subject plasma at any time with limit of detection of 5 ng/mL. In conclusion, this study suggests that t-RES from red wine is poorly bioavailable and even an important red wine consumption does not make it possible to obtain detectable plasma concentrations of t-RES.

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Determination of trans-resveratrol in human plasma by high-performance liquid chromatography

Cited by 31 publications

References 8 publications

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Quantitation of trans-resveratrol and detection of its metabolites in human plasma and urine by high performance liquid chromatography

HPLC Analysis of Trans-Resveratrol in Human Plasma After Red Wine Consumption

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