Determination of Trazodone(R) and Its Metabolite, m-CPP, in Serum and Urine by HPLC

Tella, A. Scotto di; Nunzio, Ciro Di; Ricci, Pierre-Louis; Parisi, Gregory

doi:10.1093/jat/10.6.233

Cited by 9 publications

(3 citation statements)

References 4 publications

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“…This method has a better extraction recovery percentage and a better precision than other methods for quantitative determination of trazodone in blood [15][16][17][18][19][20][21][22][23][24][25] . The proposed method has extraction recoveries of trazodone from serum between 92.5 % and 96.7 %, whereas the method by Okhubo et al 19 showed extraction recoveries of 81.0 % to 84.2 %, and the method of RaviPrakash et al 16 had trazodone recoveries from blood between 36.1% and 40.8%.…”

Section: Comparison Of Results With Other Analytical Methods To Determentioning

confidence: 99%

“…This procedure enables the determination of trazodone using a very simple and fast liquidliquid extraction method, with a very good efficacy, having extraction recoveries between 92.5 % and 96.7 %, better than other methods to quantify trazodone in blood [15][16][17][18][19][20][21][22][23][24][25] . The intra-day and inter-day variation of trazodone concentrations were in concordance with the precision criteria for an assay method for drugs in biological fluids [36][37][38][39] , and also were better than other methods to quantitative determination of trazodone in blood [15][16][17][18][19][20][21][22][23][24][25] . Serum levels of trazodone at therapeutically efective doses (0.48-1.62 ng/μL) 1-3 are above the detection limit (0.016 ng/μL) and above the quantification limit (0.048 ng/ μL) for this method.…”

Section: Discussionmentioning

confidence: 99%

“…Literature survey reveals that some LC [15][16][17][18][19][20][21][22][23] and GC 24 had been reported for the determination of trazodone in human blood in the last years. Also a HPTLC method 25 , but, although this HPTLC method was find, our work has important advantages, as its precision.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of Validated HPTLC Method for Quantification of Trazodone in Human Serum

Mennickent¹,

González²,

Sierra³

et al. 2014

J. Chil. Chem. Soc.

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A high performance thin-layer chromatographic (HPTLC) method for quantitative analysis of trazodone in human serum has been developed and validated. Trazodone was extracted with n-hexane: isoamyl alcohol (99:1). Chromatographic separation was performed on silica gel 60 F 254 HPTLC plates, with toluene: acetone: ethanol: ammonium (9:7:2:0.5, v/v/v/v) as mobile phase. Densitometric evaluation was carried out at 290 nm. There was no chromatographic interference between trazodone and its major metabolite, m-chlorophenilpiperazine (R f 0.82 and 0.39, respectively). Regression analysis of the calibration plot revealed good linearity (r=0.999) over the range of 20.00 and 200.00 ng/band, corresponding to 0.20 and 2.00 ng/μL of trazodone in human serum after extraction process and applying 10 μL to the chromatographic plates. The % RSD of intra-assay and inter-assay precision, were in the range of 0.98% to 2.97% (n=3) and 1.06% to 3.54% (n=9), respectively. LOD and LOQ were found to be 0.016ng/μL, and 0.048ng/μL, and the recovery values, of trazodone from serum, were between 92.52 % and 96.73 %. This method was successfully applied to quantify trazodone in patient serum samples. In conclusion, the developed method might be useful for the quantitative determination of trazodone in human serum, as a tool to evaluate patient adherence to their pharmacotherapy with this drug.

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