Determination of treosulfan in plasma and urine by HPLC with refractometric detection; pharmacokinetic studies in children undergoing myeloablative treatment prior to haematopoietic stem cell transplantation

Pharmacokinetics and Pharmacodynamics of Treosulfan in Patients With Thalassemia Major Undergoing Allogeneic Hematopoietic Stem Cell Transplantation

“…The interday assay precision as reported in %CV was <10% and intraday precision was <13%. The mean recovery of Treo from plasma was much higher in our assay, i.e., 63.7–81.8% as compared to a previously reported method, which was 40–48.1%. The lower limits of detection and quantification were 0.5 μg/mL and 1.0 μg/mL, respectively.…”

“…Hence, the therapeutic range of Treo to achieve better HSCT outcome has not been established so far. This is probably due to a limited number of pharmacokinetic (PK) studies of Treo conducted in the HSCT setting with a relatively small number of patients with a difference in interindividual variability (IIV) . These studies included various disease conditions, the majority being hematological malignant disorders, dose of Treo, rate of infusion, and method of Treo analysis.…”

Pharmacokinetics of Treosulfan in Pediatric Patients Undergoing Hematopoietic Stem Cell Transplantation

“…Glowka et al 11 presented methods for analyzing treosulfan in biologic fluids based on RP-HPLC with refractometric detection. Another article of Glowka et al 16 describes a HPLC method using UV detection at 264 nm.…”

“…6 Only 2 reports describe the pharmacokinetics of treosulfan in pediatric patients. 10,11 In these studies, the interpatient variability in exposure AUC of 5 children seems to be very large (CV = 70%), and therapeutic drug monitoring could therefore be of great importance to optimize treosulfan therapy for the individual patient.…”

Refractive Index Measurement of Pharmaceutical Solids: A Review of Measurement Methods and Pharmaceutical Applications

“…14 Liquid refractometry is also a very common high-performance liquid chromatography detector. [15][16][17][18][19] The wide availability and ease of use of these refractometers has expanded refractive index applications for liquid systems, ranging from counterfeit drug detection 3,5 to biomaterial analysis. 4,9 In contrast, refractive index measurements of pharmaceutical solids are more challenging owing to their complexity (i.e., dimension, solid state, crystal form, number of components, and so forth).…”