Determination of tricyclic antidepressants using silica gel with a reversed-phase eluent

Bidlingmeyer, Brian A.; Korpi, J.; Little, James N.

doi:10.1007/bf02290438

Cited by 20 publications

(1 citation statement)

References 5 publications

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“…Samples were hydrolyzed in the gas phase with 6 M hydrochloric acid containing 0.1% (by volume) phenol for 24 h at 115 ‡C under N 2 vacuum [12]. The liberated amino acids were reacted with phenylisothiocyanate and the resulting phenylthiocarbamoyl amino acids analyzed by RP-HPLC on a Nova Pak ODS column (3.9U150 mm, 4 Wm; Waters) in a Hewlett Packard liquid chromatograph 1090 with an automatic injection system [13].…”

Section: Amino Acid Analysismentioning

confidence: 99%

Functional and structural characterization of a novel member of the natriuretic family of peptides from the venom of Pseudocerastes persicus

et al. 2003

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A novel peptide, PNP (Pseudocerastes persicus natriuretic peptide), was isolated from the venom of the Iranian viper P. persicus. Amino acid sequencing revealed that the 37-residue peptide belongs to the family of natriuretic peptides. The physiological e¡ects of intra-venously PNP infused into anesthetized rats on urine £ow, sodium excretion and blood pressure were comparable to those of atrial natriuretic peptide (ANP). In PC12 cells that were treated with either PNP, ANP, or C-type natriuretic peptide, PNP induced a similar cGMP response as ANP. Since PC12 cells only express the natriuretic peptide receptor (NPR)-A receptor we conclude that PNP binds to the NPR-A receptor. The solution conformation of PNP was characterized using 1 H nuclear magnetic resonance spectroscopy and indicates a high degree of conformational £exi-bility.

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Section: Amino Acid Analysismentioning

confidence: 99%