Determination of tryptophan in unhydrolyzed food and feedstuffs by the acid ninhydrin method

Pintér-Szakács, M.; Molnár-Perl, I.

doi:10.1021/jf00093a028

Cited by 92 publications

(32 citation statements)

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“…After removal of HCl/phenol by evaporation, the amino acid compositions were established after chromatography on a Biochrom 20 System (Pharmacia). Tryptophan content was measured colorimetrically (Pintér-Szakács & Molnár-Perl 1990). Aqueous soybean crude extracts -The seeds were ground in a coffee grinder to a fine powder.…”

Section: Methodsmentioning

confidence: 99%

Brazilian soybean Glycine max (L.) Merr. cultivars adapted to low latitude regions: seed composition and content of bioactive proteins

et al. 2006

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-(Brazilian soybean Glycine max (L.) Merr. cultivars adapted to low latitude regions: seed composition and content of bioactive proteins). Among the goals of the Brazilian soybean improvement programmes, the breeding strategies for cultivars adapted to low latitudes have been included to extend crop areas and to increase production. Seeds of nine Brazilian soybean cultivars adapted to low latitudes were investigated regarding to their composition, and amino acid and antinutritional/ toxic protein contents. Protein (394.5 ± 13.1 to 445.3 ± 8.0 g kg flour) presented variations in their contents amongst the cultivars. Compared to other soybean cultivars, urease was higher, the acute toxicity lower and the lectin and trypsin inhibitor contents similar to data available. In general, soybean cultivars showed similar biochemical composition to those developed in different geographic regions. The relevance of these findings to the agronomic features and to choice of soybean cultivars to be used as food or feed is discussed.Key words -amino acid content, antinutritional factors, seed composition, soybean, toxicity RESUMO -(Cultivares brasileiras de soja Glycine max (L.) Merr. adaptadas ao cultivo em baixas latitudes: composição da semente e conteúdo de proteínas bioativas). Dentre os objetivos dos programas de melhoramento da soja no Brasil, está o desenvolvimento de estratégias para obtenção de cultivares adaptadas às regiões de baixas latitudes, visando a ampliação da área cultivada e o aumento da produção. Sementes de nove cultivares brasileiras de soja adaptadas a estas condições foram investigadas em relação à sua composição proximal e conteúdos de aminoácidos e proteínas tóxicas e/ou antinutricionais. Os conteúdos de proteína (394,5 ± 13,1 a 445,3 ± 8,0 g kg farinha). Comparados com outras cultivares de soja, os conteúdos de urease verificados foram superiores, a toxicidade aguda observada foi inferior e os conteúdos de lectina e inibidores de tripsina foram semelhantes aos resultados disponíveis na literatura. De um modo geral, as cultivares estudadas mostraram composição bioquímica similar àquela verificada para genótipos desenvolvidos em outras regiões geográficas. A relevância desses achados para as propriedades agronômicas e a seleção de cultivares de soja para a alimentação de seres humanos e animais são discutidas.Palavras-chave -composição química da semente, conteúdo de aminoácidos, fatores antinutricionais, soja, toxicidade

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Section: Methodsmentioning

confidence: 99%

Brazilian soybean Glycine max (L.) Merr. cultivars adapted to low latitude regions: seed composition and content of bioactive proteins

et al. 2006

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“…The column temperature was set at 40°C. Tryptophan content was measured by the acid ninhydrin method of Pintér-Szakács and Molnár-Perl (1990). The results were expressed as mg/g of dry weight.…”

Section: Amino Acid Compositionmentioning

confidence: 99%

Comparison of nutrient composition in kernel of tenera and clonal materials of oil palm (Elaeis guineensis Jacq.)

Kok

Ong‐Abdullah

et al. 2011

Food Chemistry

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a b s t r a c tNutritive information about oil palm kernel is scarce, especially on the composition of sugars and watersoluble vitamins. This study aims to evaluate both tenera and clonal materials for their proximate composition, fatty acid profile, amino acid composition, sugar, mineral and water-soluble vitamin contents. The tenera material had a higher moisture, fat and fibre content as compared to the clonal material, whereas protein, carbohydrate and ash content were higher in the clonal material. The major fatty acid constituents in palm kernel oil were lauric acid, myristic acid and oleic acid. The palm kernel proteins were deficient in lysine and tryptophan but rich in glutamic acid, arginine and aspartic acid. Sucrose was the most abundant sugar in palm kernel. The mineral analysis of the samples showed high levels of potassium, phosphorus, magnesium, calcium and manganese, while niacin was the water-soluble vitamin present at the highest concentrations in palm kernel.

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“…Tryptophan was estimated by the ninhydrin method of Pintér-Szakács and Molnán-Perl (1990). One gram of sample was introduced into a 25 ml polypylene test tube with caps and then 10 ml of 0.075 mol/L NaOH was added and mixed until there were no lumps.…”

Section: Determination Of Tryptophanmentioning

confidence: 99%

Nutritional evaluation of caseins and whey proteins and their hydrolysates from Protamex

Sindayikengera

Xia

2006

J. Zhejiang Univ. - Sci. B

115

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Whey protein concentrate (WPC 80) and sodium caseinate were hydrolyzed by Protamex to 5%, 10%, 15%, and 20% degree of hydrolysis (DH). WPC 80, sodium caseinate and their hydrolysates were then analyzed, compared and evaluated for their nutritional qualities. Their chemical composition, protein solubility, amino acid composition, essential amino acid index (EAA index), biological value (BV), nutritional index (NI), chemical score, enzymic protein efficiency ratio (E-PER) and in vitro protein digestibility (IVPD) were determined. The results indicated that the enzymatic hydrolysis of WPC 80 and sodium caseinate by Protamex improved the solubility and IVPD of their hydrolysates. WPC 80, sodium caseinate and their hydrolysates were high-quality proteins and had a surplus of essential amino acids compared with the FAO/WHO/UNU (1985) reference standard. The nutritive value of WPC 80 and its hydrolysates was superior to that of sodium caseinate and its hydrolysates as indicated by some nutritional parameters such as the amino acid composition, chemical score, EAA index and predicted BV. However, the E-PER was lower for the WPC hydrolysates as compared to unhydrolyzed WPC 80 but sodium caseinate and its hydrolysates did not differ significantly. The nutritional qualities of WPC 80, sodium caseinate and their hydrolysates were good and make them appropriate for food formulations or as nutritional supplements.

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Determination of tryptophan in unhydrolyzed food and feedstuffs by the acid ninhydrin method

Cited by 92 publications

References 1 publication

Brazilian soybean Glycine max (L.) Merr. cultivars adapted to low latitude regions: seed composition and content of bioactive proteins

Brazilian soybean Glycine max (L.) Merr. cultivars adapted to low latitude regions: seed composition and content of bioactive proteins

Comparison of nutrient composition in kernel of tenera and clonal materials of oil palm (Elaeis guineensis Jacq.)

Nutritional evaluation of caseins and whey proteins and their hydrolysates from Protamex

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