Determination of Tryptophan with p-Dimethylaminobenzaldehyde Using Photochemical Development of Color

Spies, Joseph R.; Chambers, Dorris C.

doi:10.1021/ac60045a040

Cited by 27 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amino acids was analyzed using Spectrophotometric method based on ninhydrin chemical reaction following the method previously described by Schroeder et al (1990), Spies and Chambers (1950), Gaitonde (1967). Typically, ninhydrin combines with amino acids to form coloured complexes, the intensity of the colours depend on the amount of amino acid present.…”

Section: Amino Acid Determinationmentioning

confidence: 99%

Amino Acid and Proximate Composition of Saccharomyces cerevisiae Biomass Cultivated in Cassava Mill Effluents

Izah¹,

Bassey²,

Ohimain³

2017

mmr

View full text Add to dashboard Cite

Cassava mill effluent (CME) is generated during cassava processing into cassava flake or gari. The effluents affects the quality of the receiving environment such soil. It is could affect domestic animals such as goat and sheep when they ingest it, and retard normal growth and productivity of plants in the affected soil. This study evaluated amino acid and proximate composition of Saccharomyces cerevisiae cultivated in cassava mill effluents. S. cerevisiae used in this study was isolated from palm wine based on cultural, morphological, and physiological/biochemical characteristics. 10 ml of S. cerevisiae broth was inoculated into 100ml of sterile cassava mill effluents filtered with double muslin cloth. The medium was shaken intermittently between 7.00 to 19.00 hours' time interval. The medium was decanted and subsequently filtered using Whatman filter paper. The resultant biomass was washed with distilled water and re-filtered. The sludge/biomass recovered were oven dried. Amino acid and proximate composition of the yeast biomass produced was analyzed using spectrophotometer. Proximate composition was also analyzed following standard procedure. Result showed that the total essential amino acid (40.88 g/100 g) was slightly higher than the Food and Agricultural Organization/World Health Organization (FAO/WHO) standard for feed use. While the total non-essential amino acid (21.56 g/100 g) was lower than FAO/WHO limits. Mean proximate composition was 17.01% (crude protein), 7.34% (ash), 56.40% (carbohydrate) and 407.13 kcal/100 g (calorific value/energy content). The findings of this study showed that during fermentation of cassava mill effluents with S. cerevisiae an appreciable amount protein is enriched considering the fact that the substrate is a waste.

show abstract

Section: Amino Acid Determinationmentioning

confidence: 99%

Amino Acid and Proximate Composition of Saccharomyces cerevisiae Biomass Cultivated in Cassava Mill Effluents

Izah¹,

Bassey²,

Ohimain³

2017

mmr

View full text Add to dashboard Cite

show abstract

“…Methionine and cysteine were determined separately using the performic acid procedure of Moore [23]. Tryptophan was determined according to the method of Spies and Chambers [24].…”

Section: Amino Acid Analysismentioning

confidence: 99%

Assessment of protein nutritional quality and effects of traditional processes: A comparison between Ethiopian quality protein maize and five Ethiopian adapted normal maize cultivars

Fufa

Akalu

Wondimu

et al. 2003

Nahrung

View full text Add to dashboard Cite

The present study was designed to quantitatively measure and compare the levels and variations of total protein, individual amino acids, and computed protein efficiency ratio (C-PER) in raw and traditionally processed products of one recently released quality protein maize (QPM BH542) with four high-yield maize hybrids, namely flint BH660, semi-dent BH140, Pioneer 30H83, and Pioneer 30G97, as well as one local maize cultivar. The total protein content was variable among the cultivars ranging from 7% for BH660 to 8.6% for Pioneer 30H83, 8.9% for BH140, 9.8% for QPM BH542, 10.1% for local maize cultivar, and 11.8% for Pioneer 30G97, respectively. However, the QPM BH542 maize protein proved to be higher in nutritional quality than common maize proteins because it contained 30% to 82% more lysine, higher levels of arginine, tryptophan, histidine, threonine, cysteine, and valine. As a result, the QPM BH542 amino acid profile gives a good balance of total essential amino acids, limited only in lysine, and has a C-PER ratio of 2.2 compared to 1.14, 1.2, 1.4, 1.66, and 1.67 for Pioneer 30G97, local, BH-140, BH660, and Pioneer 30H83, respectively. The various traditional processes of maize have no significant effect on the protein nutritional quality of the new quality protein maize. Hence, the widely dissemination of it in agricultural cultivation as well as consumption by the general population is recommended.

show abstract

“…Although the method of SPIES and CHAMBERS has been extensively used and normally gives reliable results, the method has some limitations. Variations in the absorption maximum (from a normal value of 590-600 nm to 545-460 nm) have been verified with different protein samples (379). Intense light, oxidizing and reducing agents, including H2S, bisulfite and chloride ions interfere with the method (135, 379).…”

Section: ···-Nhthco-···mentioning

confidence: 94%

“…The long known reaction of the indole nucleus of tryptophan with aldehydes under strongly acidic conditions to give colored products (135,287,397) was employed by SPIES and CHAMBERS (379) in a colorimetric procedure for the determination of tryptophan. In this method, the tryptophan-containing compounds (peptides or proteins) are dissolved in concentrated sulfuric acid containing p-dimethylaminobenzaldehyde (DAB) and a blue color is obtained after a prescribed time by adding sodium nitrite solution.…”

Section: P-dimethylaminobenzaldehydementioning

confidence: 99%

“…Intense light, oxidizing and reducing agents, including H2S, bisulfite and chloride ions interfere with the method (135, 379). Interference has been eliminated to some extent by appropriate addition of silver sulfate to the assay mixture (379). Low tryptophan values are obtained due to reaction of sulfur amino acids, serine and threonine with the indole nucleus of tryptophan.…”

Section: ···-Nhthco-···mentioning

confidence: 99%

See 1 more Smart Citation

The Chemistry of Tryptophan in Peptides and Proteins

Fontana¹,

Toniolo²

1976

Fortschritte Der Chemie Organischer Naturstoffe / Progress in the Chemistry of Organic Natural Products

View full text Add to dashboard Cite

Determination of Tryptophan with p-Dimethylaminobenzaldehyde Using Photochemical Development of Color

Cited by 27 publications

References 5 publications

Amino Acid and Proximate Composition of <i>Saccharomyces cerevisiae</i> Biomass Cultivated in Cassava Mill Effluents

Amino Acid and Proximate Composition of <i>Saccharomyces cerevisiae</i> Biomass Cultivated in Cassava Mill Effluents

Assessment of protein nutritional quality and effects of traditional processes: A comparison between Ethiopian quality protein maize and five Ethiopian adapted normal maize cultivars

The Chemistry of Tryptophan in Peptides and Proteins

Contact Info

Product

Resources

About