Determination of tulobuterol in rat plasma using a liquid chromatography–tandem mass spectrometry method and its application to a pharmacokinetic study of tulobuterol patch

Han, Xiao; Liu, Ran; Ji, Lifang; Hui, Mei; Li, Qing; Fang, Liang; Bi, Kaishun

doi:10.1016/j.jchromb.2015.11.017

Cited by 3 publications

(8 citation statements)

References 13 publications

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“…Excitingly, Cur enhanced the distribution of DOX in nucleic fraction, which was 4.12 times that of DOX-Sol after incubation for 2h. Because Cur could down-regulate P-glycoprotein expression (Choi et al, 2008;Shukla et al, 2009) and reverse MDR in resistant cells (Han et al, 2016;Meiyanto et al, 2014)…”

Section: Distribution In Subcellular Fractionsmentioning

confidence: 99%

“…Cur functions on cellular enzymes including cyclooxygenase and glutathione S-transferase, and inhibits NF-κB activity to induce tumor cell apoptosis (Choi, Kim, Lim, Abbreviations used: Cur, curcumin; DOX, doxorubicin; Gli, glibenclamide; HA-VES, hyaluronic acid-vitamin E succinate; MDR, multidrug resistance; PM, polymeric micelle. Shin, & Lee, 2008;Han et al, 2016;Meiyanto et al, 2014;Shukla et al, 2009). Meanwhile, our previous results show that Cur could improve the absorption of DOX in vivo (Ma, Wang, Guo, & Tu, 2015b).…”

Section: Introductionmentioning

confidence: 99%

“…It was rejected if the measured peak area in blank cell lysate sample was <20% of LLOQ(Han et al, 2016).Linearity was assayed by a series of standard samples with different concentrations on three consecutive days and analyzed by a weighted least-square linear regression using 1/x 2 of the peak area ratios of analytes to IS against concentrations. Selectivity was assessed by analyzing blank cell lysate in comparison with QC cell lysate samples and a sample derived from cells incubated for 1h.…”

mentioning

confidence: 99%

“…and reverse MDR in resistant cells(Han et al, 2016;Meiyanto et al, 2014), it is known to have a synergistic antitumor effect with DOX. For that reason, a combination of DOX and Cur could increase the internalization of DOX into resistant cells and increase concentration of DOX in its acting site.…”

mentioning

confidence: 99%

“…Moreover, the residual effect was investigated by spike one analyte at high concentration to examine its effect on blank cell lysate sample. It was rejected if the measured peak area in blank cell lysate sample was <20% of LLOQ(Han et al, 2016).Linearity was assayed by a series of standard samples with different concentrations on three consecutive days and analyzed by a weighted least-square linear regression using 1/x 2 of the peak area ratios of analytes to IS against concentrations. The concentrations of analytes in the QCs or test samples were calculated by the regression parameters from the calibration curves.…”

mentioning

confidence: 99%

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Validated LC–MS/MS method for simultaneous determination of doxorubicin and curcumin in polymeric micelles in subcellular compartments of MCF‐7/Adr cells by protein precipitation–ultrasonic breaking method

Wang

Liu

et al. 2016

Biomedical Chromatography

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A specific and sensitive LC-MS/MS with protein precipitation- ultrasonic breaking method has been developed and validated for simultaneous determination of doxorubicin (DOX) and curcumin (Cur) in DOX and Cur co-loaded hyaluronic acid-vitamin E succinatemicelles [(DOX + Cur)-polymeric micelles (PMs)] in subcellular compartments of resistant MCF-7/Adr cells. Sequential extraction of four subcellular protein fractions (cytosolic, membrane/organelle, nucleic and cytoskeleton) was performed directly from MCF-7/Adr cells after incubation with (DOX + Cur)-PMs. An ultrasonic breaking-methanol precipitation method was used for extraction of the fractions, and the micelle breaking efficiency with methanol was 98.1 and 97.6% for DOX and Cur, respectively. The analytes were analyzed using positive electrospray ionization coupled with multiple reaction monitoring. The calibration curves were linear over a concentration range of 0.5-400 ng/mL for DOX and 2-2000 ng/mL for Cur, and the recovery for the two analytes were >85% with negligible matrix effect. The intra-day and inter-day precision was <10.80% and relative error was within ±7.70%. The developed method was successfully applied for subcellular determination of DOX and Cur in MCF-7/Adr cells. Moreover, Cur and (DOX + Cur)-PMs had a marked promoting effect on the distribution of DOX in the nucleic protein fraction.

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Section: Distribution In Subcellular Fractionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Validated LC–MS/MS method for simultaneous determination of doxorubicin and curcumin in polymeric micelles in subcellular compartments of MCF‐7/Adr cells by protein precipitation–ultrasonic breaking method

Wang

Liu

et al. 2016

Biomedical Chromatography

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Development of Quantitative Method of Tulobuterol Hydrochloride in Rat Plasma: Validation and Application to Preclinical Pharmacokinetics

NAZ,

SUBRAHMANYAM,

RACHAMALLA

2023

Int J App Pharm

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Objective: A robust, simple, accurate, rapid, and selective bioanalytical high-performance liquid chromatography (HPLC) method was established and validated to determine the tulobuterol hydrochloride in rat plasma. Methods: The protein precipitation method deproteinated analyte from rat plasma using acetone. The analysis of tulobuterol hydrochloride from rat plasma was accomplished using a mobile phase comprising of methanol: potassium dihydrogen orthophosphate buffer (0.05M; pH 4.0) in 90:10 (v/v) ratio run at 1.0 ml/min flow rate. Separation was carried on BDS hypersil C18 column (4.6 mm × 250 mm; 5 µ) at ambient temperature employing a 996 photodiode array (PDA) detector at 228 nm. Results: The linearity model was exhibited from 100-500 ng/ml with a good correlation of 0.999. Tulobuterol hydrochloride was efficiently separated at a retention time of 7.281 min. The percent recovery rate was between 100.21-100.46 %. The accuracy, precision, robustness, and ruggedness study showed relative standard deviation (%RSD) was within 2% (acceptable limit), and that revealed the method was efficient, precise, reliable, and reproducible. Conclusion: A simple, accurate, suitable method to quantitate tulobuterol hydrochloride in rat plasma was established using HPLC employed with a PDA detector that overcomes the increased cost for analysis. The developed method was successfully validated in rat plasma.

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Synthesis of Chiral 5-Aryl-2-oxazolidinones via an Ir-BiphPHOX Catalyzed Enantioselective Hydrogenation

Zhang¹,

Yan²,

He³

et al. 2022

Chinese Journal of Organic Chemistry

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Determination of tulobuterol in rat plasma using a liquid chromatography–tandem mass spectrometry method and its application to a pharmacokinetic study of tulobuterol patch

Cited by 3 publications

References 13 publications

Validated LC–MS/MS method for simultaneous determination of doxorubicin and curcumin in polymeric micelles in subcellular compartments of MCF‐7/Adr cells by protein precipitation–ultrasonic breaking method

Validated LC–MS/MS method for simultaneous determination of doxorubicin and curcumin in polymeric micelles in subcellular compartments of MCF‐7/Adr cells by protein precipitation–ultrasonic breaking method

Development of Quantitative Method of Tulobuterol Hydrochloride in Rat Plasma: Validation and Application to Preclinical Pharmacokinetics

Synthesis of Chiral 5-Aryl-2-oxazolidinones via an Ir-BiphPHOX Catalyzed Enantioselective Hydrogenation

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