Determination of ultrasonic attenuation in small samples of solid material by scanning acoustic microscopy with phase contrast

Hillmann, K.; Grill, Wolfgang; Bereiter‐Hahn, Jürgen

doi:10.1016/0925-8388(94)90582-7

Cited by 20 publications

(11 citation statements)

References 2 publications

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“…The resonance frequency of the rod oscillation is, among other values (e.g., the density of the specimen), a function of the viscosity of the surrounding fluid. The extremely small displacements (Ͼ1 nm) of the rod are monitored by an acoustic microscope with phase-sensitive detection (Grill et al, 1996;Hillmann et al, 1994). High frequency pulses (1 GHz) are converted by a transducer into longitudinal acoustic waves, coupling into a sapphire and focused onto the free tip of the glass fiber.…”

Section: The Oscillating Rod Rheometer (Orr)mentioning

confidence: 99%

Viscoelastic Properties of f-actin, Microtubules, f-actin/α-actinin, and f-actin/Hexokinase Determined in Microliter Volumes with a Novel Nondestructive Method

Wagner

Zinke

Dancker

et al. 1999

Biophysical Journal

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A nondestructive method to determine viscoelastic properties of gels and fluids involves an oscillating glass fiber serving as a sensor for the viscosity of the surrounding fluid. Extremely small displacements (typically 1-100 nm) are caused by the glass rod oscillating at its resonance frequency. These displacements are analyzed using a phase-sensitive acoustic microscope. Alterations of the elastic modulus of a fluid or gel change the propagation speed of a longitudinal acoustic wave. The system allows to study quantities as small as 10 microliters with temporal resolution >1 Hz. For 2-100 microM f-actin gels a final viscosity of 1.3-9.4 mPa s and a final elastic modulus of 2.229-2.254 GPa (corresponding to 1493-1501 m/s sound velocity) have been determined. For 10- to 100-microM microtubule gels (native, without stabilization by taxol), a final viscosity of 1.5-124 mPa s and a final elastic modulus of 2.288-2. 547 GPa (approximately 1513-1596 m/s) have been determined. During polymerization the sound velocity in low-concentration actin solutions increased up to +1.3 m/s (approximately 1.69 kPa) and decreased up to -7 m/s (approximately 49 kPa) at high actin concentrations. On polymerization of tubulin a concentration-dependent decrease of sound velocity was observed, too (+48 to -12 m/s approximately 2.3-0.1 MPa, for 10- to 100-microM tubulin). This decrease was interpreted by a nematic phase transition of the actin filaments and microtubules with increasing concentration. 2 mM ATP (when compared to 0.2 mM ATP) increased polymerization rate, final viscosity and elastic modulus of f-actin (17 microM). The actin-binding glycolytic enzyme hexokinase also accelerated the polymerization rate and final viscosity but elastic modulus (2.26 GPa) was less than for f-actin polymerized in presence of 0.2 mM ATP (2.28 GPa).

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Section: The Oscillating Rod Rheometer (Orr)mentioning

confidence: 99%

Viscoelastic Properties of f-actin, Microtubules, f-actin/α-actinin, and f-actin/Hexokinase Determined in Microliter Volumes with a Novel Nondestructive Method

Wagner

Zinke

Dancker

et al. 1999

Biophysical Journal

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“…For the determination of the mechanical properties of microscopic objects attached to a substrate by phase contrast scanning ultrasonic microscopy (PSAM) it is necessary to use the velocity of ultrasonic waves as an input parameter for modeling 7 . Biological samples including living cells are commonly placed on microscope substrate glasses or on substrates manufactured from various polymers.…”

Section: Applicationsmentioning

confidence: 99%

Determination of the velocity of sound with high resolution by ultrasonic imaging of wedge shaped objects in transmission with vector contrast

Amjad

Ndop

Twerdowski

et al. 2008

Health Monitoring of Structural and Biological Systems 2008

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The determination of the velocity of sound for small objects suffers from limited resolution concerning as well the determination of the extension along the path of the sound waves as the determination of the time-of-flight. Imaging of planar objects with a wedge shaped boarder allows imaging in transmission with no object and the full object in the path of the acoustic waves in a continuous manner. Such phase tracking available by PSAM can be used to determine the variation of the time-of-flight with ultimate resolution. Furthermore, for a coupling fluid with a speed equal to the speed of the object under study, the extension of the object does not contribute to the result. Similarly for coupling fluids with sound velocities close to the one of the object under study the error concerning the extension which can be substantial for microscopic objects is reduced and can be minimized by selection of suitable fluids. The method is demonstrated and application involving different objects and fluids are demonstrated.

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“…Scanning acoustic microscopy (SAM) studies on thin hard films and layered systems have also been expansively reviewed in reference [4]. Phase-sensitive acoustic microscopy (PSAM) techniques [5,6] present obvious advantages over standard SAM. In this study, we employ high frequency PSAM (GHz regime) on standard polymer film (soft matter) systems.…”

Section: Introductionmentioning

confidence: 99%

Phase-sensitive acoustic microscopy of polymer thin films

2004

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Determination of ultrasonic attenuation in small samples of solid material by scanning acoustic microscopy with phase contrast

Cited by 20 publications

References 2 publications

Viscoelastic Properties of f-actin, Microtubules, f-actin/α-actinin, and f-actin/Hexokinase Determined in Microliter Volumes with a Novel Nondestructive Method

Viscoelastic Properties of f-actin, Microtubules, f-actin/α-actinin, and f-actin/Hexokinase Determined in Microliter Volumes with a Novel Nondestructive Method

Determination of the velocity of sound with high resolution by ultrasonic imaging of wedge shaped objects in transmission with vector contrast

Phase-sensitive acoustic microscopy of polymer thin films

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