Determination of Underivatized Polyamines: A Review of Analytical Methods and Applications

AL-Hadithi, Nabil N.; Saad, Bahruddin

doi:10.1080/00032719.2010.551686

Cited by 14 publications

(6 citation statements)

References 62 publications

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“…Gas chromatography, thin-layer chromatography and high-performance liquid chromatography have been applied for the analysis of polyamines in food (85–87). Concretely, high or ultra high-performance liquid chromatography with ion-exchange columns or reverse-phase columns to separate polyamines are the most frequently reported techniques in the literature (88).…”

Section: Introductionmentioning

confidence: 99%

“…Post-column derivatization has the advantage that it is automatically performed online, thereby avoiding sample manipulation and shortening the time required for the analysis (89). In recent years, the determination of polyamines through liquid chromatography coupled to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) has emerged as an alternative analytical technique, very specific and sensitive and without the need of derivatization (52, 87, 88, 90).…”

Section: Introductionmentioning

confidence: 99%

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Polyamines in Food

et al. 2019

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The polyamines spermine, spermidine, and putrescine are involved in various biological processes, notably in cell proliferation and differentiation, and also have antioxidant properties. Dietary polyamines have important implications in human health, mainly in the intestinal maturation and in the differentiation and development of immune system. The antioxidant and anti-inflammatory effect of polyamine can also play an important role in the prevention of chronic diseases such as cardiovascular diseases. In addition to endogenous synthesis, food is an important source of polyamines. Although there are no recommendations for polyamine daily intake, it is known that in stages of rapid cell growth (i.e., in the neonatal period), polyamine requirements are high. Additionally, de novo synthesis of polyamines tends to decrease with age, which is why their dietary sources acquire a greater importance in an aging population. Polyamine daily intake differs among to the available estimations, probably due to different dietary patterns and methodologies of data collection. Polyamines can be found in all types of foods in a wide range of concentrations. Spermidine and spermine are naturally present in food whereas putrescine could also have a microbial origin. The main polyamine in plant-based products is spermidine, whereas spermine content is generally higher in animal-derived foods. This article reviews the main implications of polyamines for human health, as well as their content in food and breast milk and infant formula. In addition, the estimated levels of polyamines intake in different populations are provided.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polyamines in Food

et al. 2019

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“…Hyphenated techniques, such as LC/MS, GC/MS or capillary electrophoreses coupled to MS are among the most powerful methods for the analysis of metabolites [19–24] . Nonetheless, many published analytical methods suffer from at least one of the following drawbacks: they have long chromatographic run times; they are optimized for determination of polyamines only in one specific matrix; they have higher limits of detection; or they require time-consuming sample pretreatment steps [1,2,16,21,22,24–32] .…”

Section: Introductionmentioning

confidence: 99%

Polyamines in biological samples: Rapid and robust quantification by solid-phase extraction online-coupled to liquid chromatography–tandem mass spectrometry

Magnes

Fauland

Gander

et al. 2014

Journal of Chromatography A

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“…To assay the polyamine contents, classical analytical techniques such as chromatographic techniques coupled with different detectors, capillary electrophoresis, or immunoassays are still primarily used nowadays . Most of these techniques are expensive, time‐consuming, and require experienced technical staffs to handle their operation and tedious sample‐pretreatment or derivatization steps.…”

Section: Introductionmentioning

confidence: 99%

“…Most of these techniques are expensive, time‐consuming, and require experienced technical staffs to handle their operation and tedious sample‐pretreatment or derivatization steps. Development of fast kits towards these chromophore‐free analytes is therefore a hot area of research in analytical fields …”

Section: Introductionmentioning

confidence: 99%

Study of the Aggregation of DNA‐Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Tsoi

et al. 2017

ChemPlusChem

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As mart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in ac onvenient and fast manner. Acomprehensive study was performed to elucidate the driving force of DNA adsorption,d ifferent factors' effects, such as gold nanoparticle size, DNA length, concentration, and working pH towardss permine sensing by using UV/Vis absorption spectroscopya nd isothermalt itration calorimetry.I tw as found that the developed aptasensor could detectspermine by two differ-ent sensing mechanismss imply by adjustingt he DNA concentration without complicated procedures. Good performance in complicated matrices was provenb yt he satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such af lexible and smart approach described here would provide au seful tool for the fast sensing of spermine and prostatec ancer screening.[a] T.

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Determination of Underivatized Polyamines: A Review of Analytical Methods and Applications

Cited by 14 publications

References 62 publications

Polyamines in Food

Polyamines in Food

Polyamines in biological samples: Rapid and robust quantification by solid-phase extraction online-coupled to liquid chromatography–tandem mass spectrometry

Study of the Aggregation of DNA‐Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

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