Determination of Urea in Serum Based on the Combination of an Enzymatic Reaction with Immobilized Urease and Ion Chromatographic Analysis

Miyauchi, Toshiyuki; Miyachi, Yusuke; Takahashi, Makoto; Ishikawa, Norihisa; Mori, Hidehiko

doi:10.2116/analsci.26.847

Cited by 12 publications

(4 citation statements)

References 21 publications

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“…1.0 and 5.0 lM urea were determined six times, with RSD of 3.5 and 2.6%, respectively. This method had good selectivity and sensitivity comparing with the reported method (Table 1) [1][2][3][4][5][21][22][23][24] and was one of the most sensitive methods for urea.…”

Section: Working Curvementioning

confidence: 86%

A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catalytic reaction and NH4 + associated particle reaction

Liang

Qin

Zhang

et al. 2011

Bioprocess Biosyst Eng

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In the pH 6.6 Na2HPO4-NaH2PO4 buffer solutions and in the presence of urease catalyst, urea can be decomposed to form NH4+. The NH4+ reacted with sodium tetraphenyl boron (NaTPB) to form the association particles that exhibited a resonance scattering (RS) peak at 474 nm. When the urea concentration increased, NH4+ increased, and RS intensity at 474 nm enhanced linearly. Under the chosen conditions, the increased RS intensity (ΔI (474 nm)) had a linear response to the urea concentration in the range of 0.125-15 μM, with a detection limit of 0.058 μM urea, and a regression equation of ΔI (474 nm) = 31.6C + 2.1, a correlation coefficient of 0.9986. This catalytic RS method was applied for the detection of urea in human serum sample, with good selectivity and sensitivity, and the results were consistent with the reference method.

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Section: Working Curvementioning

confidence: 86%

A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catalytic reaction and NH4 + associated particle reaction

Liang

Qin

Zhang

et al. 2011

Bioprocess Biosyst Eng

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“…In recent years, different analytical methods such as spectrophotometric method [5,[15][16][17] ion chromatography [9,18], high performance liquid chromatography [3,[19][20][21][22][23], gas chromatography mass spectrometry [24,25] reversed-phase liquid chromatography with tandem mass spectrometry [26], high-performance liquid chromatography/isotope dilution mass spectrometry [27], liquid chromatography-tandem mass spectrometry [2,28] and chemiluminescence [29], liquid chromatography/mass spectrometry [30] for estimation of urea, creatinine and UA levels in human serum or plasma samples, as well as urine have been developed. This study aims to develop new and very simple methods to solve overlapping spectra in their ternary mixtures without preliminary separation steps.…”

Section: Introductionmentioning

confidence: 99%

Ratio derivative-zero crossing and successive derivative of ratio spectra for simultaneous determination of urea, creatinine, and uric acid in human urine samples

Ali¹,

Fakhre²,

Rasheed³

2023

Bull. Chem. Soc. Eth.

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ABSTRACT. Two simple precise and accurate spectrophotometric techniques are developed for simultaneous determination of ternary mixture of urea, creatinine, and uric acid in humane urine sample. The first technique is a ratio derivative zero - crossing where measured urea at 216.61 nm, creatinine at 260.56 nm, and uric acid at 283 nm. The second technique is a successive derivative of ratio spectra where urea, creatinine, and uric acid measured at 203 nm, 261 nm, and 283 nm, 287 nm, respectively. These procedures do not need separation. The proposed methods showed excellent linear range over the concentration ranges of 1.0–15.0 μg/mL, 1.0–16.0 μg/mL, and 2.0–15.0 μg/mL for urea, creatinine, and uric acid, respectively. The recoveries ranged from 97.10% to 101.9% for urea, 97.22% to102.70 % for creatinine, and 97.45% to102.55 % for uric acid with relative standard deviations less than 1.56% for urea, 3.87% for creatinine, and 3.71% for uric acid. The analytical eco-scale and green analytical procedure index tools were used to evaluate how the proposed procedures will affect the environment. The simultaneous quantification of urea, creatinine, and uric acid in human urine samples can be accomplished with remarkable effectiveness by using the suggested approaches. KEY WORDS: Urea, Creatinine, Uric acid, Derivative spectrophotometry Bull. Chem. Soc. Ethiop. 2023, 37(4), 817-829. DOI: https://dx.doi.org/10.4314/bcse.v37i4.2

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“…However, these detection methods often have many disadvantages such as time-consuming detection and complex pretreatment process. The commonly used methods for urea detection include the electrochemical sensing method [12], flow injection method [13], ion chromatography [14], high performance liquid chromatography [15], and fluorescence spectroscopy [16], among others. These traditional methods are usually expensive and require complex instruments, as well as time-consuming and complex sample pretreatment process, making them unsuitable for field monitoring.…”

Section: Introductionmentioning

confidence: 99%

An Endoscope-like SERS Probe Based on the Focusing Effect of Silica Nanospheres for Tyrosine and Urea Detection in Sweat

et al. 2022

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In this work, we developed a new type of SERS probe, which was composed of glass-SiO2-Au@MBN@Ag nanoparticles (NPs) three-dimensional Surface-enhanced Raman spectroscopy (SERS) substrate. When the laser passed through the quartz glass sheet, on the one hand, the SiO2 NPs supporting the Au@MBN@Ag NPs increase the roughness of the substrate surface, resulting in a large number of hot spots among nanoparticles. On the other hand, based on the focusing effect of silicon dioxide nanospheres, the laser can better focus on the surface of nanoparticles in the inverted SERS probe, thus showing better SERS enhancement. Furthermore, the Au@MBN@Ag NPs core-shell structure was used with 4-mercaptobenzoonitrile (MBN) as an internal standard molecule, and the quantitative determination of tyrosine and urea was realized by internal standard correction method. The standard working curves of the two had good linear correlation with R2 above 0.9555. The detection limits of tyrosine and urea were in the range of 2.85 × 10−10 M~7.54 × 10−6 M, which confirms that this design can be used for quantitative and specific detection of biological molecules, demonstrating great practical significance for the research of diseases such as skin lesions and endocrine disorders.

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Determination of Urea in Serum Based on the Combination of an Enzymatic Reaction with Immobilized Urease and Ion Chromatographic Analysis

Cited by 12 publications

References 21 publications

A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catalytic reaction and NH4 + associated particle reaction

A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catalytic reaction and NH4 + associated particle reaction

Ratio derivative-zero crossing and successive derivative of ratio spectra for simultaneous determination of urea, creatinine, and uric acid in human urine samples

An Endoscope-like SERS Probe Based on the Focusing Effect of Silica Nanospheres for Tyrosine and Urea Detection in Sweat

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