Determination of Urease Biochemical Properties of Asparagus Bean (<i>Vigna unguiculata ssp sesquipedalis</i> L.)

Zusfahair,; Ningsih, Dian Riana; Fatoni, Amin; Pertiwi, Darul Santri

doi:10.1088/1757-899x/349/1/012073

Cited by 8 publications

(11 citation statements)

References 6 publications

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“…Nilai pH lingkungan dapat mempengaruhi keefektifan sisi aktif enzim dalam membentuk kompleks enzim dengan substrat (16) . Dengan demikian kenaikan konsentrasi sel isolat bakteri berperan untuk mempercepat proses terjadinya degradasi urea karena adanya enzim urease pada isolat bakteri tersebut yang menjadi pemeran utama dalam proses biogrouting.…”

Section: H2co3unclassified

“…Kenaikan pH tertinggi pada penelitian ini ditunjukkan oleh isolat bakteri K5A. Nilai kenaikan pH pada isolat bakteri K5A sebesar 3,27 yang berarti nilai ini lebih besar daripada kenaikan pH pada penelitian lain, yaitu sebesar 2 (16) . Kenaikan pH yang berbeda dapat disebabkan oleh sumber isolat dan strain bakteri yang berbeda.…”

Section: H2co3unclassified

“…Oleh karena itu, dari perbedaan ini juga dapat dihasilkan karakteristik enzim urease yang berbeda atau asam amino penyusun enzim yang berbeda sehingga pengaruh terhadap pH juga akan memberikan perbedaan pada aktivitas enzimnya. Karakteristik enzim yang berbeda dapat berasal dari perubahan tingkat ionisasi dalam enzim atau substrat yang berbeda sehingga akan mempengaruhi aktivitas dan ketika terjadi kenaikan pH konformasi enzim berada dalam kondisi ideal yang menyebabkan interaksi antara enzim dan substrat menjadi maksimal dalam proses degradasi urea yang membentuk produk (amonia) (16) .…”

Section: H2co3unclassified

“…Nilai ini sebanding dengan nilai kenaikan pH pada isolat bakteri K5A yang lebih tinggi daripada isolat bakteri K7. Pada penelitian lain menunjukkan ketika pH 5 aktivitas urease yang dihasilkan hanya 70%, namun ketika pH 7 aktivitas urease meningkat menjadi 100%; dengan demkian kenaikan pH sebesar 2 dapat menaikkan konsentrasi amonia hingga 30% (16) . Dengan kata lain, kenaikan pH yang tinggi menghasilkan tingginya kenaikan konsentrasi amonia, sedangkan saat kenaikan pH lebih rendah menyebabkan konsentrasi amonia yang dihasilkan tidak maksimal.…”

Section: H2co3unclassified

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Isolasi dan Produktivitas Bakteri Ureolitik dari Sedimen Muara Sungai Citarum

2020

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This research carried out isolation of ureolytic bacteria taken from soil samples from the mangrove area of Muara Gembong, Citarum River. The aim was to characterize and to test the urease production activity of the ureolytic bacteria. The study was conducted at the BPPT Environmental Laboratory in Building 820 Geostech, Puspiptek Setu, South Tangerang City, Banten Province, from April until September 2019. The results showed that there were ureolytic bacterial isolates from soil samples taken. The productivity of ureolytic bacterial isolates was indicated by the increasing ammonia concentration and the increase of bacterial cell concentration supported by the pH and temperature factors per volume unit of 10 ml. The increase in ammonia concentration of the six isolates’ test media showed significant differences. The highest increase in ammonia concentration was 15.19 ppm produced by isolate K5A and the second highest was 14.10 ppm by isolate K7. Another productivity testing was performed by determining if there was an increase in the concentration of bacterial cells. Based on the analysis of variance, each isolates significantly affected the increase in bacterial cell concentration. The highest increase in bacterial cell concentration by 250.45×106 CFU/ml occurred in isolates K5A, while isolate K7 indicated the second-highest increase in cell concentration by 233.31×106 CFU/ml.Keywords: ureolytic bacteria, productivity, mangrove sediment, Citarum River EstuaryABSTRAK Penelitian ini melakukan isolasi bakteri ureolitik yang diambil dari sampel tanah dari daerah mangrove dari di Muara Gembong, Sungai Citarum. Tujuannya adalah untuk melakukan karakterisasi dan menguji aktivitas produksi urease terhadap isolat bakteri ureolitik yang ditemukan. Penelitian dilakukan di Laboratorium Lingkungan BPPT di Gedung 820 Geostech, Puspiptek Setu, Kota Tangerang Selatan, Provinsi Banten mulai bulan April sampai dengan September 2019. Enam isolat bakteri dapat diperoleh dari sampel tanah yang diambil. Produktivitas isolat bakteri ureolitik pada penelitian ini dapat ditandai dengan kenaikan konsentrasi amonia kenaikan konsentrasi sel bakteri yang didukung dengan faktor pH dan suhu per satuan volume 10 ml. Kenaikan konsentrasi amonia dari keenam media uji isolat menunjukkan perbedaan yang nyata. Produksi konsentrasi amonia tertinggi sebesar 15,19 ppm dihasilkan oleh isolat K5A dan tertinggi kedua sebesar 14,10 ppm oleh isolat K7. Untuk uji produktivitas juga dilakukan dengan melihat adanya kenaikan konsentrasi sel bakteri. Berdasarkan analisis variansi, masing-masing isolat berpengaruh nyata terhadap kenaikan konsentrasi sel bakteri. Kenaikan konsentrasi sel bakteri tertinggi sebesar 250,45×106 CFU/ml terjadi pada isolat bakteri K5A sedangkan isolat K7 menunjukkan kenaikan konsentrasi sel tertinggi kedua sebesar 233,31×106 CFU/ml.Kata kunci: bakteri ureolitik, produktivitas, sedimen mangrove, Muara Sungai Citarum

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Section: H2co3unclassified

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Isolasi dan Produktivitas Bakteri Ureolitik dari Sedimen Muara Sungai Citarum

2020

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“…Urease is most commonly used as the biosensing element in the urea biosensor study. Urease could be obtained from microorganism (Smith, King, & Goodman, 2009) or beans, such as asparagus bean (Zusfahair, Ningsih, Fatoni, & Santri, 2018a); (Zusfahair, Ningsih, Fatoni, & Santri, 2018b), black-eyed pea (Zusfahair, Ningsih, Fatoni, & Putri, 2018), cotton seed (Menegassi et al, 2008) and jack bean (Follmer, Wassermann, & Carlini, 2004).…”

Section: Introductionmentioning

confidence: 99%

Development of Urea Biosensor Based on Immobilized Urease in Chitosan Cryogel

Zusfahair

Ningsih

Lestari

et al. 2019

Molekul

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The development of biosensors using biological components has an important role in detecting the disease early because it has good selectivity and accuracy. In this study, a biosensor which made is a urea biosensor, based on immobilization urease in chitosan using adsorption techniques, to measure urea levels by colorimetric analysis with bromothymol blue (BTB) as an indicator. The purpose of this study was to find out how to measure urea levels using biosensors based on urease immobilization in chitosan and find out the biosensor performance including optimum enzymatic reaction time, linearity, the limit of detection, repetition, and determination of disrupting compounds. The study began with the making of an immobilization supporting matrix using chitosan which was made in the form of cryogel through an ionic gelation process which adsorbs the urease enzyme. Cryogel urease catalyzes the hydrolysis of urea into NH4+ and CO2-. The reaction product was added with the BTB indicator, and the color change formed was measured using a spectrophotometer. The results showed that the performance of urea biosensors was good enough for urea level detection systems by producing enzymatic reaction times at 15 minutes, linearity at 0.9951, detection limit at 0.018 mM, not affected by the addition of 0.05 mM ascorbic acid and 0.4 mM uric acid. This urea biosensor can be used up to 5 repetitions.

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Benzimidazolone‐piperazine/triazole/thiadiazole/furan/thiophene conjugates: Synthesis, in vitro urease inhibition, and in silico molecular docking studies

Güven,

Menteşe,

Emirik

et al. 2023

Archiv der Pharmazie

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This study describes the synthesis, in vitro urease inhibition, and molecular docking studies of benzimidazolone derivatives incorporating the piperazine, triazole, thiadiazole, furan, thiophene, and thiosemicarbazide moieties. All newly synthesized compounds demonstrated varying degrees of urease inhibitory activity, with IC50 values ranging between 0.64 ± 0.099 and 0.11 ± 0.017 µM, when compared with the standard drug thiourea (IC50 value of 0.51 ± 0.028 µM). To confirm the experimental urease inhibition results and elucidate the mode of interaction of the synthesized compounds with the binding site of the urease enzyme, molecular docking studies were performed using the Schrödinger Suite package. Molecular docking studies showed that compounds with high in vitro urease inhibition interacted with key residues of the urease active site such as His221, Glu222, Asp223, His322, Arg338, and Ni2+ cations via hydrogen bonding, metal coordination, salt bridge, π–π stacking, and π–cation interactions.

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Determination of Urease Biochemical Properties of Asparagus Bean (Vigna unguiculata ssp sesquipedalis L.)

Cited by 8 publications

References 6 publications

Isolasi dan Produktivitas Bakteri Ureolitik dari Sedimen Muara Sungai Citarum

Isolasi dan Produktivitas Bakteri Ureolitik dari Sedimen Muara Sungai Citarum

Development of Urea Biosensor Based on Immobilized Urease in Chitosan Cryogel

Benzimidazolone‐piperazine/triazole/thiadiazole/furan/thiophene conjugates: Synthesis, in vitro urease inhibition, and in silico molecular docking studies

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