Determination of urinary cortisol and 6β-hydroxycortisol by high performance liquid chromatography

Nakamura, Jiro; Yakata, M

doi:10.1016/0009-8981(85)90335-3

Cited by 23 publications

(9 citation statements)

References 21 publications

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“…The h.p.l.c. method we used was validated on the basis of the following points: (1) Our data for healthy adults were in good agreement with previously published values (Dumont et al, 1984;Nakamura & Yakata, 1985;Hosoda et al, 1981;Kishida & Fukushima, 1983;Nahoul et al, 1982;Park, 1978;Zhiri et al, 1986;Desage et al, 1987). (2) In addition we had the opportunity, in a separate study, to perform both our h.p.l.c.…”

Section: Other Monooxygenase Assayssupporting

confidence: 79%

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The increase in urinary excretion of 6 beta‐hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction.

Ged

Rouillon

Pichard

et al. 1989

Brit J Clinical Pharma

312

156

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1. Urinary excretion of 6 beta‐hydroxycortisol, hepatic microsomal cortisol 6 beta‐hydroxylase and the specific content of several forms of cytochrome P450 were measured in 8 to 14 patients before and after treatment with rifampicin (600 mg orally per day for 4 days). 2. Rifampicin treatment produced an average five fold increase in daily excretion of urinary 6 beta‐hydroxycortisol. 3. Cortisol 6 beta‐ hydroxylase activity increased from 15 +/‐ 6 pmol min‐1 mg‐1 in organ donors (considered as 'control subjects') to 87 +/‐ 31 pmol min‐1 mg‐1 in rifampicin treated patients. 4. Among three forms of human P450 (P450IA, IIC and IIIA), (1), (2), measured by Western blots, only P450IIIA was significantly induced by the antibiotic. 5. Only antibodies against P450IIIA selectively inhibited cortisol 6 beta‐ hydroxylase in human liver microsomes. 6. Cortisol 6 beta‐hydroxylase was correlated with P450IIIA specific content. 7. The urinary level of 6 beta‐hydroxycortisol correlated with liver microsomal cortisol 6 beta‐ hydroxylase and P450IIIA specific content. 8. We conclude that P450IIIA is predominantly responsible for cortisol 6 beta‐hydroxylase activity in human liver microsomes and that urinary 6 beta‐hydroxycortisol is a marker of the induction of this cytochrome P450.

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Section: Other Monooxygenase Assayssupporting

confidence: 79%

“…Various h.p.l.c. systems, as well as immunoassays, have been described for this measurement (Dumont et al, 1984;Nakamura & Yakata, 1985;Hosoda et al, 1981;Kishida & Fukushima, 1983;Nahoul et al, 1982;Park, 1978;Zhiri et al, 1986;Desage et al, 1987). The h.p.l.c.…”

Section: Other Monooxygenase Assaysmentioning

confidence: 99%

The increase in urinary excretion of 6 beta‐hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction.

Ged

Rouillon

Pichard

et al. 1989

Brit J Clinical Pharma

312

156

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“…We tested several compounds for use as internal standards such as 6␤-hydroxytestosterone, dexamethasone [26,27], methylprednisolone [33], and beclomethsone for the HPLC analysis of 6␤-OHF and cortisol. Dexamethasone has been widely used as the internal standard [26,27], but an interfering peak was observed at t R 28.80 min derived from urine (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The HPLC method generally offers higher selectivity in measuring urinary 6␤-OHF [23][24][25][26][27][28][29][30], but the results are not often reproducible in quantitation of 6␤-OHF in urine, because of polar interferences and low recoveries of 6␤-OHF.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of 6β-hydroxycortisol and cortisol in human urine by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity determined by the cortisol 6β-hydroxylation clearance

Furuta

Mori

Suzuki

et al. 2004

Journal of Chromatography B

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Urinary 6β-hydroxycortisol excretion in Hong Kong Chinese patients with hepatocellular carcinoma and other chronic liver diseases

Young

Critchley

et al. 1996

Cancer

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Our results show an increase in mean CYP3A4 enzyme activity, reflected as an increase in the 6 beta OHF/F ratio, in Hong Kong Chinese with nonresectable HCC compared with those with resectable HCC and other liver diseases. Although the role of increased CYP3A4 activity in the aetiology of HCC is not known, our specificity and sensitivity estimates suggest that a high 6 beta OHF/F ratio indicates probable inoperability. However, a normal level is a poor predictor of resectability.

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Determination of urinary cortisol and 6β-hydroxycortisol by high performance liquid chromatography

Cited by 23 publications

References 21 publications

The increase in urinary excretion of 6 beta‐hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction.

The increase in urinary excretion of 6 beta‐hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction.

Simultaneous determination of 6β-hydroxycortisol and cortisol in human urine by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity determined by the cortisol 6β-hydroxylation clearance

Urinary 6β-hydroxycortisol excretion in Hong Kong Chinese patients with hepatocellular carcinoma and other chronic liver diseases

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