Determination of urinary malondialdehyde by isotope dilution LC-MS/MS with automated solid-phase extraction: A cautionary note on derivatization optimization

Chen, Jian‐Lian; Huang, Yen‐Hua; Pan, Chih-Hong; Hu, Chiung‐Wen; Chao, Mu‐Rong

doi:10.1016/j.freeradbiomed.2011.08.012

Cited by 53 publications

(41 citation statements)

References 32 publications

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“…Compared with other methods, the LOD for the urine samples was similar to values obtained using HPLC–FLD and LC–MS/MS , which require SPE and preconcentration, as described in Table . The LOD of the proposed method was approximately 50–100 times lower than the LODs for methods using HPLC after derivatization with thiobarbituric acid and 2,4‐dinitrophenylhydrazine in urine samples .…”

Section: Resultssupporting

confidence: 67%

“…In these methods, malondialdehyde was derivatized using the reagents 2,4,6‐trichlorophenylhydrazine , phenylhydrazine , pentafluorophenylhydrazine , and 2,2,2‐trifluoroethylhydrazine . HPLC methods have also been developed using diode array detection , fluorescence detection , and MS/MS . Malondialdehyde can also be derivatized using diaminonaphthalene , hydralazine , thiobarbituric acid , 2,4‐dinitrophenylhydrazine , 9‐fluorenylmethoxycarbonylhydrazine , 2‐aminoacridone , 2,2‐furil , 4‐(2‐trimethylammonio)ethoxy benzenaminium halide , and dansylhydrazine .…”

Section: Introductionmentioning

confidence: 99%

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Simple and sensitive determination of malondialdehyde in human urine and saliva using UHPLC–MS/MS after derivatization with 3,4‐diaminobenzophenone

Shin

2017

J of Separation Science

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Malondialdehyde has been used as a biomarker for lipid peroxidation in biological samples. An ultra-high performance liquid chromatography with tandem mass spectrometry method was developed to determine the levels of malondialdehyde in human urine and saliva samples. To select the optimum derivatization reagent from four diamino compounds, the reactivity and sensitivity of their derivatives were compared, and 3,4-diaminobenzophenone was selected. The optimum reaction conditions for malondialdehyde with 3,4-diaminobenzophenone were as follows: a reagent dosage of 50 mg/L, pH of 4, and reaction for 30 min at 50°C. The formed derivative product was analyzed using ultra-high performance liquid chromatography with tandem mass spectrometry without additional extraction or concentration steps. In the optimal conditions, the method was used to determine malondialdehyde concentration in human urine and saliva samples. The limits of quantification for malondialdehyde in biological samples were over a concentration range of 0.1-0.3 μg/L. Additionally, the calibration curve showed a linearity greater than r = 0.997. The method was used to analyze 14 human urine and saliva samples from healthy volunteers. Malondialdehyde was detected in the concentration range of 1.7-33.6 μg/g creatinine in all human urine samples and 0.1-1.3 μg/L in all human saliva samples.

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Section: Resultssupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Simple and sensitive determination of malondialdehyde in human urine and saliva using UHPLC–MS/MS after derivatization with 3,4‐diaminobenzophenone

Shin

2017

J of Separation Science

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“…ROS can react with double bonds of polyunsaturated fatty acids (PUFAs) to yield lipid hydroperoxides. Malondialdehyde is the secondary oxidation product of peroxidized PUFAs and has been shown to have mutagenic and cytotoxic effects and possibly to be involved in the pathogenesis of several human diseases, including atherosclerosis, neurodegenerative diseases, and cancer [67]. Lipid peroxidation products, namely, malondialdehyde [68], 4-hydroxynonenal (HNE) [69], advanced lipoxidation end products (ALE) [70], hydroxyoctadecadienoic acid (HODE) [71], F 2 -isoprostanes [72] (enzymatically produced by free-radical-catalyzed peroxidation of arachidonoyl lipids), and isolevuglandins, have been reported to be elevated in HD patients [73].…”

Section: Biomarkers Of Oxidative Stress and Nucleic Acid Oxidationmentioning

confidence: 99%

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Sung

Hsu

Chen

et al. 2013

Oxidative Medicine and Cellular Longevity

162

110

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Patients with chronic kidney disease (CKD) have high cardiovascular mortality and morbidity and a high risk for developing malignancy. Excessive oxidative stress is thought to play a major role in elevating these risks by increasing oxidative nucleic acid damage. Oxidative stress results from an imbalance between reactive oxygen/nitrogen species (RONS) production and antioxidant defense mechanisms and can cause vascular and tissue injuries as well as nucleic acid damage in CKD patients. The increased production of RONS, impaired nonenzymatic or enzymatic antioxidant defense mechanisms, and other risk factors including gene polymorphisms, uremic toxins (indoxyl sulfate), deficiency of arylesterase/paraoxonase, hyperhomocysteinemia, dialysis-associated membrane bioincompatibility, and endotoxin in patients with CKD can inhibit normal cell function by damaging cell lipids, arachidonic acid derivatives, carbohydrates, proteins, amino acids, and nucleic acids. Several clinical biomarkers and techniques have been used to detect the antioxidant status and oxidative stress/oxidative nucleic acid damage associated with long-term complications such as inflammation, atherosclerosis, amyloidosis, and malignancy in CKD patients. Antioxidant therapies have been studied to reduce the oxidative stress and nucleic acid oxidation in patients with CKD, including alpha-tocopherol, N-acetylcysteine, ascorbic acid, glutathione, folic acid, bardoxolone methyl, angiotensin-converting enzyme inhibitor, and providing better dialysis strategies. This paper provides an overview of radical production, antioxidant defence, pathogenesis and biomarkers of oxidative stress in patients with CKD, and possible antioxidant therapies.

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“…Additionally, hydrogen peroxide has the ability to induce lipid peroxidation, causing degradation to polyunsaturated lipids, which can cause the subsequent rupture to cell membranes [131]. The oxidation of polyunsaturated lipids results in the reactive electrophile bi-product, malondialdehyde (MDA), which is commonly used as an indicator of oxidative stress [132,133] and can be measured accurately in urine samples [134]. Others have correlated increasing abundance of urinary F 2 -isoprostane in a healthy male cohort who performed a series of multi-joint exercises at varying degrees of intensity from 'light' to 'over-training' [117].…”

Section: Oxidative Stress Markers and Post-translational Modificationsmentioning

confidence: 99%

Urinary biomarkers of physical activity: candidates and clinical utility

Sampson¹,

Broadbent²,

Parker³

et al. 2013

Expert Review of Proteomics

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Chronic physical inactivity is a major risk factor for a number of important lifestyle diseases, while inappropriate exposure to high physical demands is a risk factor for musculoskeletal injury and fatigue. Proteomic and metabolomic investigations of the physical activity continuum - extreme sedentariness to extremes in physical performance - offer increasing insight into the biological impacts of physical activity. Moreover, biomarkers, revealed in such studies, may have utility in the monitoring of metabolic and musculoskeletal health or recovery following injury. As a diagnostic matrix, urine is non-invasive to collect and it contains many biomolecules, which reflect both positive and negative adaptations to physical activity exposure. This review examines the utility and landscape of biomarkers of physical activity with particular reference to those found in urine.

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Determination of urinary malondialdehyde by isotope dilution LC-MS/MS with automated solid-phase extraction: A cautionary note on derivatization optimization

Cited by 53 publications

References 32 publications

Simple and sensitive determination of malondialdehyde in human urine and saliva using UHPLC–MS/MS after derivatization with 3,4‐diaminobenzophenone

Simple and sensitive determination of malondialdehyde in human urine and saliva using UHPLC–MS/MS after derivatization with 3,4‐diaminobenzophenone

Oxidative Stress and Nucleic Acid Oxidation in Patients with Chronic Kidney Disease

Urinary biomarkers of physical activity: candidates and clinical utility

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