Determination of vanilmandelic acid in urine by coupled-column liquid chromatography combining affinity to boronate and separation by anion exchange

Eriksson, Britt‐Marie; Wikström, Margareta

doi:10.1016/0378-4347(91)80303-t

Cited by 7 publications

(2 citation statements)

References 16 publications

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“…Other ligands besides antibodies, particularly boronates, have been shown to be valuable in performing on-line affinity extraction with HPLC. Examples include several separation methods in which boronate columns have been combined with HPLC columns for the clinical analysis of catechol-related compounds such as epinephrine, norepinephrine, and dopamine (118 -120 ), dihydroxyphenylalanine (121 ), dihydroxyphenylacetic acid (121,122 ), 5-Scysteinyldopa (123 ), and vanillylmandelic acid (124 ). This same approach has been adapted for profiling (125 ) and quantifying ribonucleotides in urine (126,127 ) and serum (127 ).…”

Section: On-line Affinity Extractionmentioning

confidence: 99%

Affinity Chromatography: A Review of Clinical Applications

Hage

1999

Clinical Chemistry

229

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Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the use of boronate, lectin, protein A or protein G, and immunoaffinity supports for the direct quantification of solutes. Newer techniques that use antibody-based columns for on- or off-line sample extraction are examined in detail, as are methods that use affinity chromatography in combination with other analytical methods, such as reversed-phase liquid chromatography, gas chromatography, and capillary electrophoresis. Indirect analyte detection methods are also described in which immunoaffinity chromatography is used to perform flow-based immunoassays. Other applications that are reviewed include affinity-based chiral separations and the use of affinity chromatography for the study of drug or hormone interactions with binding proteins. Some areas of possible future developments are then considered, such as tandem affinity methods and the use of synthetic dyes, immobilized metal ions, molecular imprints, or aptamers as affinity ligands for clinical analytes.

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Section: On-line Affinity Extractionmentioning

confidence: 99%

Affinity Chromatography: A Review of Clinical Applications

Hage

1999

Clinical Chemistry

229

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“…Using boronic acid affinity chromatography, several important biological molecules have been analyzed and purified, including carbohydrates [51], catecholamines [52][53], ribonucleosides [54], proteins [55] and steroids [56][57].…”

Section: Applications Of Boronic Acidsmentioning

confidence: 99%