Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

Schmidt, Mark S.; Huang, Rong; Classon, Robert J.; Murry, Daryl J.

doi:10.1016/j.jpba.2005.11.039

Cited by 19 publications

(24 citation statements)

References 21 publications

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“…12,26,27 In one report, however, atmospheric pressure chemical ionization (APCI) for actinomycin-D analysis was used and it was concluded that ion intensities for both ESI and APCI are of the same order of magnitude. 33 Also for vincristine both APCI 23,24 and ESI 21,26,27 have been described. Our triple quadrupole mass spectrometer is equipped with an ESI, APCI and a heated electrospray ionization (H-ESI) source.…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…12 For bioanalytical quantification of vincristine methods have been described using a radioimmunoassay, 6 HPLC coupled to UV detection, 13,14 electrochemical detection (ECD), [15][16][17][18][19] electrospray ionization (ESI) mass spectrometry 20,21 or atmospheric pressure chemical ionization (APCI) mass spectrometry. [22][23][24] Due to high sensitivity and selectivity liquid chromatography coupled on-line to triple quadrupole tandem mass spectrometry (LC/MS/MS) has become the first choice for the analysis of anticancer drugs in biological matrices. 25 To date two LC/MS/MS methods have been described quantifying actinomycin-D and vincristine simultaneously.…”

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Validated assay for the simultaneous quantification of total vincristine and actinomycin‐D concentrations in human EDTA plasma and of vincristine concentrations in human plasma ultrafiltrate by high‐performance liquid chromatography coupled with tandem mass spectrometry

Damen

Israëls

Caron

et al. 2009

Rapid Comm Mass Spectrometry

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A sensitive, specific and efficient high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) assay for the simultaneous determination of total vincristine and actinomycin-D concentrations in human plasma and an assay for the determination of unbound vincristine are presented. Electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and heated electrospray ionization (H-ESI) were tested as ionization interfaces. For reasons of robustness ESI was chosen followed by tandem mass spectrometry (ESI-MS/MS). For the plasma assay a 30 microL aliquot was protein precipitated with acetonitrile/methanol (50:50, v/v) containing the internal standard vinorelbine and 10 microL volumes were injected onto the HPLC system. To determine unbound vincristine, ultrafiltrate was produced from plasma using 30 kDa centrifugal filter units. The plasma ultrafiltrate was mixed with methanol (50:50, v/v), internal standard vinorelbine was added and 20 microL aliquots were injected onto the HPLC system. Separation was achieved on a 50x2.1 mm i.d. Xbridge C18 column using 1 mM ammonium acetate/acetonitrile (30:70, v/v) adjusted to pH 10.5 with ammonia, run in a gradient with methanol at a flow rate of 0.4 mL/min. HPLC run time was 6 min. The assay quantifies in plasma vincristine from 0.25 to 100 ng/mL and actinomycin-D from 0.5 to 250 ng/mL using plasma sample volumes of only 30 microL. Vincristine in plasma ultrafiltrate can be quantified from 1 to 100 ng/mL. Validation results demonstrate that vincristine and actinomycin-D can be accurately and precisely quantified in human plasma and plasma ultrafiltrate with the presented methods. The assays are now in use to support clinical pharmacological studies in children treated with vincristine and actinomycin-D.

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Section: Mass Spectrometrymentioning

confidence: 99%

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confidence: 99%

Validated assay for the simultaneous quantification of total vincristine and actinomycin‐D concentrations in human EDTA plasma and of vincristine concentrations in human plasma ultrafiltrate by high‐performance liquid chromatography coupled with tandem mass spectrometry

Damen

Israëls

Caron

et al. 2009

Rapid Comm Mass Spectrometry

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“…Reversed-phase applications are most often described. Schmidt et al (2006) used Strata-X columns for the bioanalysis of vincristine in plasma. Strata-X is a polymeric material with reversed-phase properties.…”

Section: Sample Pre-treatmentmentioning

confidence: 99%

“…These ionization methods can handle LC eluent flow rates up to 1 mL/min without the need for a prior eluent split. Atmospheric pressure chemical ionization (APCI) is only described for vincristine and vinblastine (Corona et al, 2008;Ramirez et al, 1997;Schmidt et al, 2006). APCI ionization is based on a principle generating ions via chemical ionization in the gas phase (Bruins, 1994).…”

Section: Detectionmentioning

confidence: 99%

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High‐performance liquid chromatography coupled with mass spectrometry for the quantitative analysis of vinca‐alkaloids in biological matrices: a concise survey from the literature

Damen

Rosing

Schellens

et al. 2009

Biomedical Chromatography

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The bioanalysis of vinca-alkaloids has been investigated extensively. High-performance liquid chromatography coupled to ultraviolet, fluorescence or electrochemical detection have been described. During recent years liquid chromatography coupled with mass spectrometry (LC-MS) has become the first choice for the quantitative bioanalysis of the vinca anticancer agents. This paper reviews recent methods for the bio-analysis of vinca-alkaloids using LC-MS, supplemented with our own experience. We will focus on sample pre-treatment, chromatography and MS detection and pay attention to problems which can occur during the bioanalysis of vinca-alkaloids. These problems encounter carry-over and absorption effects and solutions will be provided how to circumvent these problems.

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Current literature in mass spectrometry

2007

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Reviews; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (6 Weeks journals ‐ Search completed at 15th. Nov. 2006)

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Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

Cited by 19 publications

References 21 publications

Validated assay for the simultaneous quantification of total vincristine and actinomycin‐D concentrations in human EDTA plasma and of vincristine concentrations in human plasma ultrafiltrate by high‐performance liquid chromatography coupled with tandem mass spectrometry

Validated assay for the simultaneous quantification of total vincristine and actinomycin‐D concentrations in human EDTA plasma and of vincristine concentrations in human plasma ultrafiltrate by high‐performance liquid chromatography coupled with tandem mass spectrometry

High‐performance liquid chromatography coupled with mass spectrometry for the quantitative analysis of vinca‐alkaloids in biological matrices: a concise survey from the literature

Current literature in mass spectrometry

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