Determination of vinpocetine and its primary metabolite, apovincaminic acid, in rat plasma by liquid chromatography–tandem mass spectrometry

Xia, Hongmei; Su, Linlin; Guo, Jin; Pang, Zhiqing; Jiang, Xinguo

doi:10.1016/j.jchromb.2010.05.029

Cited by 21 publications

(22 citation statements)

References 24 publications

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“…Similarly, ions at m/z 236.1439 and 222.1277 were formed by loss of CO 2 from m/z 280.1347 and 266.1189, respectively. The ions at m/z 208.1128 and 193.0941 were produced by consecutive losses of C 2 H 4 and CH 3 from m/z 236.1439 …”

Section: Resultsmentioning

confidence: 99%

Identification, characterization and distribution of terpene indole alkaloids in ethanolic extracts of Catharanthus roseus using high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and the study of their geographical variation

Kumar

Bajpai

Singh

et al. 2018

Rapid Comm Mass Spectrometry

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Section: Resultsmentioning

confidence: 99%

Identification, characterization and distribution of terpene indole alkaloids in ethanolic extracts of Catharanthus roseus using high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and the study of their geographical variation

Kumar

Bajpai

Singh

et al. 2018

Rapid Comm Mass Spectrometry

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“…Stock solution stability was performed at room temperature and at 4°C by comparing the area response of a stability sample of analytes and internal standard with the area response of sample prepared from fresh stock solutions. The results should be within the acceptable limit of ±10% change for the stock solution stability experiment (Xia et al ., ). Bench‐top stability of extracted samples, room temperature stability, refrigerated stability of extracted sample at 4°C, freeze–thaw stability and long term stability at −20°C were performed at 5.00, 100 and 750 ng/mL using five replicates at each concentration.…”

Section: Methodsmentioning

confidence: 97%

Study the pharmacokinetics of AV‐45 in rat plasma and metabolism in liver microsomes by ultra‐performance liquid chromatography with mass spectrometry

Yin

Zhou

Qiao

et al. 2011

Biomedical Chromatography

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An ultra-performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed and validated to determine AV-45 in rat plasma. After the addition of the internal standard benzophenone, plasma samples were pretreated by protein precipitation. Chromatographic separation was achieved on an Acquity UPLC BEH C₁₈ column (50 × 2.1 mm, 1.7 µm) by gradient elution at a flow rate of 0.4 mL/min. Detection of analytes and internal standard (IS) was done by tandem mass spectrometry, operating in positive-ion and multiple reaction monitoring mode. The method was fully validated for its sensitivity, selectivity, accuracy and precision, matrix effect and stability study. The calibration curve showed good linearity over the concentration range 2.00-1000 ng/mL for AV-45. Intra- and inter-day precisions were less than 7.6%, and accuracy ranged from 100.6 to 107.8%. There was no matrix effect. The validated method was successfully applied to a pharmacokinetic study of AV-45 in rats. Additionally, the metabolism of AV-45 in rat liver microsomes was also studied by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC/TOF-MS). With the help of chromatographic behavior and accurate mass measurements, the metabolites were characterized.

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“…The characteristics of the proposed method have been compared to earlier reported methods. Jiang et al [14] reported a LC-MS-MS method using MRM by positive ionization mode to determine VP and AVA in rat plasma. In their paper, the procedure of sample preparation was multistep and costly by SPE, and conditions of LC separation and MS quantification were very different from n is the number of the sample in a batch, N is the batch number those in our work.…”

Section: Comparisons With Other Methodsmentioning

confidence: 99%

“…AVA was detectable by MS not only in negative mode, but also in positive mode [13,14]. In this proposal, the response in positive mode was more acute than that in negative mode using mobile containing acetonitrile water with formic acid.…”

Section: Ms-ms Conditionsmentioning

confidence: 99%

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Simultaneous Estimation of Vinpocetine and Its Metabolite, Apovincaminic Acid, in Beagle Plasma by LC-MS-MS

Yang

Lou

Gong³

et al. 2011

Chromatographia

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A method was developed to determine vinpocetine and its metabolite, apovincaminic acid, in beagle plasma by LC-MS-MS. After protein precipitation with methanol, the supernatant of the sample was concentrated and injected into an Agilent Zorbax XDB-C 18 column. The sample was separated by a mobile phase consisting of acetonitrile and 0.2% formic acid solution, and the reading was determined on an Agilent 6410 Triple Quad Tandem mass spectrometer in multiple reaction monitoring mode with the following transitions: m/z 351.5 ? 280.2/266.3 for vinpocetine, 323.2 ? 236.1/280.2 for apovincaminic acid, and 411.2 ? 191.1 for the internal standard. The intra-and inter-day variances were less than 15% (RSD%), and average recoveries were higher than 80%. The linearity ranges (LR) between 0.1 and 20.0 ng mL -1 for vinpocetine (r 2 = 0.9980) and between 1.0 and 200.0 ng mL -1 for apovincaminic acid (r 2 = 0.9995) were established. In summary, this method is sensitive, specific, and appropriate for in vivo study of various dosage forms of vinpocetine.

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Determination of vinpocetine and its primary metabolite, apovincaminic acid, in rat plasma by liquid chromatography–tandem mass spectrometry

Cited by 21 publications

References 24 publications

Identification, characterization and distribution of terpene indole alkaloids in ethanolic extracts of Catharanthus roseus using high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and the study of their geographical variation

Identification, characterization and distribution of terpene indole alkaloids in ethanolic extracts of Catharanthus roseus using high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and the study of their geographical variation

Study the pharmacokinetics of AV‐45 in rat plasma and metabolism in liver microsomes by ultra‐performance liquid chromatography with mass spectrometry

Simultaneous Estimation of Vinpocetine and Its Metabolite, Apovincaminic Acid, in Beagle Plasma by LC-MS-MS

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