Determination of vitamin C in fruits and vegetables by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry

刘真真,; Liu, Zhenzhen; 齐沛沛,; Qi, Peipei; 王新全,; Wang, Xinquan; 陈文学,; Chen, Wenxue; 吴莉宇,; Wu, Lei; 王强,; Wang, Qiang

doi:10.3724/sp.j.1123.2016.07015

Cited by 4 publications

(3 citation statements)

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“…Reduced AsA and total AsA contents were measured as described previously (Liu et al ., 2016). In brief, 0.5 g freeze‐dried sample powders were extracted with 10 ml 1% oxalic acid at 4°C for 12 h and centrifuged (14 000 g , 10 min, 4°C).…”

Section: Methodsmentioning

confidence: 99%

A comprehensive metabolic map reveals major quality regulations in red‐flesh kiwifruit (Actinidia chinensis)

Shu

Zhang

et al. 2023

New Phytologist

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Summary Kiwifruit (Actinidia chinensis) is one of the popular fruits world‐wide, and its quality is mainly determined by key metabolites (sugars, flavonoids, and vitamins). Previous works on kiwifruit are mostly done via a single omics approach or involve only limited metabolites. Consequently, the dynamic metabolomes during kiwifruit development and ripening and the underlying regulatory mechanisms are poorly understood. In this study, using high‐resolution metabolomic and transcriptomic analyses, we investigated kiwifruit metabolic landscapes at 11 different developmental and ripening stages and revealed a parallel classification of 515 metabolites and their co‐expressed genes into 10 distinct metabolic vs gene modules (MM vs GM). Through integrative bioinformatics coupled with functional genomic assays, we constructed a global map and uncovered essential transcriptomic and transcriptional regulatory networks for all major metabolic changes that occurred throughout the kiwifruit growth cycle. Apart from known MM vs GM for metabolites such as soluble sugars, we identified novel transcription factors that regulate the accumulation of procyanidins, vitamin C, and other important metabolites. Our findings thus shed light on the kiwifruit metabolic regulatory network and provide a valuable resource for the designed improvement of kiwifruit quality.

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Section: Methodsmentioning

confidence: 99%

A comprehensive metabolic map reveals major quality regulations in red‐flesh kiwifruit (Actinidia chinensis)

Shu

Zhang

et al. 2023

New Phytologist

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“…A variety of analytical methods are used to determine AA: titrimetric [2,3], spectrophotometric [2][3][4], chromatographic [5][6][7][8], fluorimetric [9], chemiluminescent and electrochemical methods [10][11][12]. Traditional methods of redox titration do not allow a quantitative assessment of the content of ascorbic acid in stained samples.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensor Based on a Carbon Veil Modified by Phytosynthesized Gold Nanoparticles for Determination of Ascorbic Acid

Брайнина

Bukharinova

Stozhko

et al. 2020

Sensors

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An original voltammetric sensor (Au-gr/CVE) based on a carbon veil (CV) and phytosynthesized gold nanoparticles (Au-gr) was developed for ascorbic acid (AA) determination. Extract from strawberry leaves was used as source of antioxidants (reducers) for Au-gr phytosynthesis. The sensor was characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy and electrochemical methods. Optimal parameters of AA determination were chosen. The sensor exhibits a linear response to AA in a wide concentration range (1 μM–5.75 mM) and a limit of detection of 0.05 μM. The developed sensor demonstrated a high intra-day repeatability of 1 μM AA response (RSD = 1.4%) and its stability during six weeks, selectivity of AA determination toward glucose, sucrose, fructose, citric, tartaric and malic acids. The proposed sensor based on Au-gr provides a higher sensitivity and a lower limit of AA detection in comparison with the sensor based on gold nanoparticles synthesized by the Turkevich method. The sensor was successfully applied for the determination of AA content in fruit juices without samples preparation. The recovery of 99%–111% and RSD no more than 6.8% confirm the good reproducibility of the juice analysis results. A good agreement with the potentiometric titration data was obtained. A correlation (r = 0.9867) between the results of AA determination obtained on the developed sensor and integral antioxidant activity of fruit juices was observed.

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“…Based on the CFs at m/z 569.3799 and 455.3496, the compound was preliminarily determined to be an OLE-type saponin. Combining the mass spectrometry and the remaining fragment ion information (Table2), compound 13 was identified as ginsenoside Ro[27][28][29]. e fragmentation of ginsenoside Ro occurred as follows: when the molecular ion at m/z 955.4875 [M-H] − lost one molecular glucose residue (162 Da), fragment ion peaks were generated at m/z 793.4306, when the molecular ions lost one CO 2 molecule (44 Da), one H 2 O (18 Da), and two molecular glucose residues (162 Da + 162 Da), the product ion peak at m/z 569.3799 was produced.…”

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confidence: 99%

Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS

Liu

Zhang

Yang

et al. 2022

Journal of Analytical Methods in Chemistry

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Saponins are the main active components in Panax ginseng C. A. Mey. (PG), Panax quinquefolius L. (PQ), and Panax japonicus C. A. Mey. (PJ), which belong to the genus Panax in the Araliaceae family. Because the chemical components in the three species are similar, they are often mixed and misused in functional foods and pharmaceuticals applications. Therefore, it is urgent to establish a method to quickly distinguish among PG, PQ, and PJ. Ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was combined with data postprocessing to identify the main characteristic fragments (CFs) and the related neutral losses (NLs) of protopanaxadiol (PPD), protopanaxatriol (PPT), oleanolic acid (OLE), and ocotillol- (OCO-) type saponins. By comparing the mass spectral data, it was possible to rapidly classify and identify saponins in PG, PQ, and PJ. A total of twenty-three chemical components were identified in the PG samples, twenty-three components were identified in the PQ samples, and twenty-seven components were identified in the PJ samples. Among them, OCO-type saponins were characteristic of PQ and PJ. Ginsenoside Rf, which was absent from PQ, allowed for differentiation between PQ and PJ. The CFs and NLs in the mass spectra of the characteristic components of PG, PQ, and PJ allowed for the rapid classification and identification of these species. Additionally, these results provide technical support for the quality evaluation of Chinese herbal medicine and for constructing a scientific regulatory system.

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Determination of vitamin C in fruits and vegetables by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry

Cited by 4 publications

References 0 publications

A comprehensive metabolic map reveals major quality regulations in red‐flesh kiwifruit (Actinidia chinensis)

A comprehensive metabolic map reveals major quality regulations in red‐flesh kiwifruit (Actinidia chinensis)

Electrochemical Sensor Based on a Carbon Veil Modified by Phytosynthesized Gold Nanoparticles for Determination of Ascorbic Acid

Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS

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