Determination of vitamin K homologues by high-performance liquid chromatography with on-line photoreactor and peroxyoxalate chemiluminescence detection

Ahmed, Sameh; Kishikawa, Naoya; Nakashima, Kenichiro; Kuroda, Naotaka

doi:10.1016/j.aca.2007.03.061

Cited by 56 publications

(49 citation statements)

References 29 publications

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“…The proposed HPLC-CL method was 2-1000 times more sensitive compared with the reported methods [21][22][23][24][25][26][27][28] and with similar sensitivity to LC-MS method [29]. However, the simplicity and low cost make the developed method more superior to LC-MS method in routine assay of vitamin K homologues.…”

Section: Resultsmentioning

confidence: 95%

“…The proposed method was found to be 15-1000 times more sensitive than HPLC-UV methods [19][20] method [28] and with sensitivity similar to LC-MS method [29]. However, the high cost and the need for technical experience make LC-MS less convenient for routine assay of vitamin K in plasma of RA patients.…”

Section: Validation Of the Proposed Methodsmentioning

confidence: 99%

“…To date, several publications were reported for the separation and determination of vitamins K in biological samples including high performance liquid chromatography (HPLC) with ultraviolet (UV) [19][20], fluorescence [21][22][23][24][25], electrochemical [26][27], chemiluminescence (CL) [28] and mass spectrometric detection [29]. In initial study to detect vitamin K homologues in plasma of RA patients, it was found that vitamin K concentrations were significantly lower in RA patients that could not be detected by many of the reported methods [19][20][21][22][23][24][25][26][27][28] while others remain expensive techniques for routine assay of vitamin K concentrations [29].…”

Section: Introductionmentioning

confidence: 99%

“…In initial study to detect vitamin K homologues in plasma of RA patients, it was found that vitamin K concentrations were significantly lower in RA patients that could not be detected by many of the reported methods [19][20][21][22][23][24][25][26][27][28] while others remain expensive techniques for routine assay of vitamin K concentrations [29]. Therefore, it is necessary to develop a highly sensitive and selective determination method designated for measurements of lower concentrations vitamin K homologues in the presence of any existed interferences from medications or plasma components.…”

Section: Introductionmentioning

confidence: 99%

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Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Ahmed

Kishikawa

Ohyama

et al. 2011

Talanta

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Vitamin K is a fat-soluble vitamin involved in blood coagulation and bone metabolism. The detection and monitoring of vitamin K homologues in rheumatoid arthritis (RA) patients is a challenging problem due to the smaller concentrations of vitamin K and the presence of several interfering medications. Therefore, this study aimed to develop a new highly sensitive and selective chemiluminescence (CL) method designated to quantify vitamin K homologues in plasma of RA patients selectively including phylloquinone (PK, vitamin K 1 ), menaquinone-4 (MK-4, vitamin K 2 ) and menaquinone-7 (MK-7, vitamin K 2 ). The method was based on the unique photochemical properties of vitamin K homologues that were exploited for selective luminol CL reaction. The correlation coefficients of 0.998 or more were obtained in the concentration ranges of 0.1-100 ng mL -1 vitamin K homologues. The detection limits were 0.03-0.1 ng mL -1 in human plasma for vitamin K homologues. The developed HPLC-CL system was successfully applied for selective determination of vitamin K homologues in plasma of RA patients. The developed method may provide a useful tool for monitoring vitamin K homologues in different clinical studies such as RA, osteoporosis and hepatocellular carcinoma in which vitamin K is intervented.

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Section: Resultsmentioning

confidence: 95%

Section: Validation Of the Proposed Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Ahmed

Kishikawa

Ohyama

et al. 2011

Talanta

Self Cite

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“…The PO-CL is easily combined with HPLC and applied to highly sensitive determination of fluorescent compounds and hydrogen peroxide. Previously, we had reported the determination of quinones by HPLC-PO-CL detection, without addition of hydrogen peroxide and fluorophore, combined with on-line photodegradation reaction [31] and this method was further adopted for the determination of vitamin K homologues [32].…”

Section: Doxorubicin (Dxr) Is a Cytotoxic Anthracycline Antibiotic Thmentioning

confidence: 99%

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Ahmed

Kishikawa

Ohyama

et al. 2009

Talanta

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A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-L was sufficient. Besides, it can detect accurately the low plasma concentrations.The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7-102.4 % and the inter-and intra-assay RSD values in rat plasma were 2.5-8.9 %. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single dose of DXR.

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Naphthoquinones and Anthraquinones: Chemical, Analytical, and Biological Overview

El‐Najjar

Gali‐Muhtasib

Vuorela

et al. 2014

Encyclopedia of Analytical Chemistry

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Naphthoquinones and anthraquinones are a well‐studied class of molecules that play a role in preventing and treating various illnesses. A thorough literature search shows that these molecules are potent antifungal, anticancer, antidiabetes, and neuroprotective agents. In addition to their beneficial properties, naphtho‐ and anthraquinones have toxicological effects mainly due to their presence as photoproducts in air pollutants. The analytical detection of naphtho‐ and anthraquinones is problematic because of the following properties: first is their ability to undergo fast redox cycling and second is their potential to bind thiol, amine, and hydroxyl groups. The possibility of using labeled compounds along with the recent advances of the available analytical techniques facilitated their detection and allowed a better understanding of their mechanism of action. This chapter summarizes the current knowledge with respect to the biological activities of naphtho‐ and anthraquinones as well as their chemical reactivity in an attempt to understand the mechanism by which they exert their effects. An overview about their analytical detection is also presented.

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Determination of vitamin K homologues by high-performance liquid chromatography with on-line photoreactor and peroxyoxalate chemiluminescence detection

Cited by 56 publications

References 29 publications

Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Naphthoquinones and Anthraquinones: Chemical, Analytical, and Biological Overview

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