Determination of whole-body calcium in vivo in rats by neutron activation analysis

Schubiger, P. August; Jakob, K; Ferreira-Marques, F.M.; Hefti, E.; Trechsel, U.; Mühlbauer, R. C.; Fleisch, H.

doi:10.1016/0047-0740(80)90006-6

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…Cesana et al (1 978) found greater flux nonunifomities than those detected in our system with the wax reflector. Schubiger et al (1980) measured flux gradients that were sufficiently large to require rotation of the rat during irradiation to lower the repositioning error.…”

Section: Discussionmentioning

confidence: 99%

The precision and accuracy of noninvasive measurements of total body calcium in the rat

Beachey¹,

Wickham²,

Webber³

1985

Can. J. Physiol. Pharmacol.

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It has been proposed that the adult castrated male rat may be used as a model of human postmenopausal osteoporosis. If such a model is valid then the total body calcium of castrated rats should progressively decrease compared with normal rats. To search for this age-related difference, it will be necessary to use a measurement technique with a precision which is small compared with the magnitude of the change to be detected. From measurements of total body calcium by neutron activation and by whole body ashing of eight dead rats, it has been shown that total body calcium can be measured with an accuracy and precision which should allow distinction between the expected lifetime changes in normal and castrated rats.

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Section: Discussionmentioning

confidence: 99%

The precision and accuracy of noninvasive measurements of total body calcium in the rat

Beachey¹,

Wickham²,

Webber³

1985

Can. J. Physiol. Pharmacol.

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“…The technique is described in detail elsewhere [8]. Whole body calcium determinations were performed with neutron activation, the rats being irradiated with thermal neutron produced by a nuclear reactor, and the calcium y-rays measured with a NaI crystal scintillation counter.…”

Section: Calcium Determinationmentioning

confidence: 99%

Use of dermestid beetles for cleaning bones

et al. 1980

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Various parts of the skeleton of normal and osteoporotic rats were compared with respect to their dry weight, ash weight, and calcium content when the bones were cleaned by Dermestes maculatus beetles or manually. Both techniques gave similar results. This was also true when whole body calcium measured by neutron activation and total skeletal calcium from bones cleaned by the beetles were compared. Thus dermestid beetles are useful as a technique to clean bones, especially for the parts of the skeleton which are difficult to dissect by hand.

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Sequential and precise in vivo measurement of bone mineral density in rats using dual-energy x-ray absorptiometry

Ammann

Rizzoli

Slosman

et al. 1992

Journal of Bone and Mineral Research

199

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In the design of new strategies for the treatment of osteoporosis, noninvasive, precise, and sensitive bone mass measurement capable of detecting changes over short periods of time in small animals is essential. Most of the models described thus far require the sacrifice of the animals and/or display low reproducibility. Using a dual-energy x-ray absorptiometer (DEXA; Hologic QDR-1000) in an ultrahigh-resolution mode, we measured bone mineral density (BMD) in rats at the levels of lumbar spine (L1-4), proximal tail (caudal vertebrae C2-4), and tibia. Accuracy was evaluated by measuring the mineral content of bone powder capsules (within the range of rat vertebrae BMD), under 0.5-3 cm water to mimic variations in soft tissue thickness. The bone powder capsule mineral content was highly correlated with chemically determined hydroxyapatite content (r = 0.999). In vivo reproducibility was evaluated by calculating the coefficient of variation (CV = 100 x SD/mean) of four to six BMD measurements, each time with repositioning, in seven rats (220-500 g body weight). CV was 1.36 +/- 0.32% (x +/- SD) for lumbar spine, 0.66 +/- 0.50% for proximal tail, and 1.12 +/- 0.45% for tibia. The ability to detect BMD changes was investigated by measuring BMD before and every 4 weeks after ovariectomy (OVX) in 270 g rats, pair fed during the whole experiment. Compared with sham-operated control animals, a highly significant difference in lumbar spine BMD was observed 4 weeks after OVX, which reached a maximum by 8 weeks and remained stable thereafter. At the level of the proximal tibia, the difference was maximal 4 weeks after OVX.(ABSTRACT TRUNCATED AT 250 WORDS)

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Determination of whole-body calcium in vivo in rats by neutron activation analysis

Cited by 5 publications

References 4 publications

The precision and accuracy of noninvasive measurements of total body calcium in the rat

The precision and accuracy of noninvasive measurements of total body calcium in the rat

Use of dermestid beetles for cleaning bones

Sequential and precise in vivo measurement of bone mineral density in rats using dual-energy x-ray absorptiometry

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