Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo™3

Chen, Jun; Zheng, Liang; Lawrence, Theodore S.; Smith, David E.

doi:10.1016/j.jchromb.2005.02.003

Cited by 12 publications

(1 citation statement)

References 15 publications

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“…Fluorescent reagents such as monobromobimane (MBB) and 4-fluoro-7-sulfobenzofurazan (SBD-F) are not attractive due to the requirement of elevated temperatures and long reaction times [8,9]. Ellman's, 4,4 0 -dithiodipyridine (DTDP) and Thioglo s -3 react faster with thiols but the first two suffer from undesirable side reactions while the high cost of Thioglo s -3 limits its applicability to routine analyses [10][11][12]. O-Phthaldialdehyde (OPA), that is a common derivatizing reagent for primary amines, can also be used for the indirect fluorimetric or spectrophotometric determination of thiols [13].…”

Section: Introductionmentioning

confidence: 99%

Automated pre‐column derivatization of thiolic fruit‐antibrowning agents by sequential injection coupled to high‐performance liquid chromatography using a monolithic stationary phase and an in‐loop stopped‐flow approach

Karakosta

Tzanavaras

Themelis

2011

J of Separation Science

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The present study reports the very first application of ethyl propiolate (EP) as an advantageous pre-column derivatization reagent for the determination of thiols by liquid chromatography (LC). Cysteine (CYS), glutathione (GSH) and N-acetylcysteine (NAC) were derivatized online under stopped-flow conditions in a sequential injection (SI) system coupled to HPLC. The formed derivatives were separated isocratically with a monolithic stationary phase (100×4.6 mm id) and UV detected at 285 nm. Critical parameters that affected the online pre-column derivatization reaction (e.g. the reaction time and the amount concentration of EP) and the separation (e.g. pH and the composition of the mobile phase) were investigated. The developed analytical scheme offers a total analysis time of less than 10 min, limits of detection in the range of 0.24-0.35 μmol/L and satisfactory linearity up to 200 μmol/L for all analytes. The proposed method was applied to the analysis of the selected thiols--that are often employed as antibrowning agents--in fresh fruit samples.

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Section: Introductionmentioning

confidence: 99%

Automated pre‐column derivatization of thiolic fruit‐antibrowning agents by sequential injection coupled to high‐performance liquid chromatography using a monolithic stationary phase and an in‐loop stopped‐flow approach

Karakosta

Tzanavaras

Themelis

2011

J of Separation Science

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Determination of the active and inactive metabolites of prasugrel in human plasma by liquid chromatography/tandem mass spectrometry

Farid

McIntosh

Garofolo

et al. 2006

Rapid Comm Mass Spectrometry

116

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Two fast and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS)-based bioanalytical assays were developed and validated to quantify the active and three inactive metabolites of prasugrel. Prasugrel is a novel thienopyridine prodrug that is metabolized to the pharmacologically active metabolite in addition to three inactive metabolites, which directly relate to the formation and elimination of the active metabolite. After extraction and separation, the analytes were detected and quantified using a triple quadrupole mass spectrometer using positive electrospray ionization. The validated concentration range for the inactive metabolites assay was from 1 to 500 ng/mL for each of the three analytes. Additionally, a 5x dilution factor was validated. The interday accuracy ranged from -10.5% to 12.5% and the precision ranged from 2.4% to 6.6% for all three analytes. All results showed accuracy and precision within +/-20% at the lower limit of quantification and +/-15% at other levels. The validated concentration range for the active metabolite assay was from 0.5 to 250 ng/mL. Additionally, a 10x dilution factor was validated. The interbatch accuracy ranged from -7.00% to 5.98%, while the precision ranged from 0.98% to 3.39%. Derivatization of the active metabolite in blood with 2-bromo-3'-methoxyacetophenone immediately after collection was essential to ensure the stability of the metabolite during sample processing and storage. These methods have been applied to determine the concentrations of the active and inactive metabolites of prasugrel in human plasma.

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Silver nanoparticles conjugated MnFe-based Prussian blue analogue for voltammetric and impedimetric bioaptasensing of amifostine (ethyol)

Alkahtani

2020

Microchim Acta

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Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo™3

Cited by 12 publications

References 15 publications

Automated pre‐column derivatization of thiolic fruit‐antibrowning agents by sequential injection coupled to high‐performance liquid chromatography using a monolithic stationary phase and an in‐loop stopped‐flow approach

Automated pre‐column derivatization of thiolic fruit‐antibrowning agents by sequential injection coupled to high‐performance liquid chromatography using a monolithic stationary phase and an in‐loop stopped‐flow approach

Determination of the active and inactive metabolites of prasugrel in human plasma by liquid chromatography/tandem mass spectrometry

Silver nanoparticles conjugated MnFe-based Prussian blue analogue for voltammetric and impedimetric bioaptasensing of amifostine (ethyol)

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