Determination of Yeast Carbohydrates with the Anthrone Reagent

Trevelyan, W. E.; Forrest, Robert; Harrison, Joseph S.

doi:10.1038/170626a0

Cited by 142 publications

(83 citation statements)

References 3 publications

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“…Fractions were monitored by UV absorption at 214, 254, and 280 nm, by native PAGE with Stains-All detection reagent and with colorimetric assays to detect the presence of carbohydrates (anthrone (28) and deoxy-sugar (29)). 2-Fold diluted individual SEC pools were subjected to anion exchange column chromatography (AEC) with two 5-ml HiTrap Q HP in series equilibrated with 25 mM Tris, 50 mM NaCl.…”

Section: Methodsmentioning

confidence: 99%

“…Fractions were monitored by absorption at 280 nm. Fractions corresponding to the conjugate (positive for the presence of both protein and polysaccharide) were pooled, and the conjugate was characterized by the determination of the carbohydrate and protein content using anthrone (28) 13 C). All two-dimensional experiments were performed using standard pulse sequences, DQCOSY, TOCSY (mixing time ϭ 120 ms), ROESY (mixing time ϭ 500 ms), and 1 H- 13 C HSQC/HMBC (long range relay transfer delay ϭ 100 ms).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A Vaccine Approach for the Prevention of Infections by Multidrug-resistant Enterococcus faecium

Kodali

Vinogradov

Lin

et al. 2015

Journal of Biological Chemistry

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Background: E. faecium infections are increasingly refractory to antibiotics, encouraging vaccine approaches. Results: Structures of novel protective surface polysaccharides and a teichoic acid were identified from a multidrug-resistant strain. Conclusion: Detection of these antigens on diverse clinical strains predicts broad coverage for a potential multivalent vaccine. Significance: Results suggest the feasibility of a glycoconjugate vaccine to prevent E. faecium disease.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Vaccine Approach for the Prevention of Infections by Multidrug-resistant Enterococcus faecium

Kodali

Vinogradov

Lin

et al. 2015

Journal of Biological Chemistry

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“…The total sugar content was determined by the Antrone method [21], and expressed as Glucose Equivalents.…”

Section: Methodsmentioning

confidence: 99%

Recovery and Concentration of Antioxidants from Winery Wastes

et al. 2012

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Grape and wine byproducts have been extensively studied for the recovery of phenolic compounds with antioxidant activity and a variety of biological actions. The selective recovery and concentration of the phenolic compounds from the liquid phase separated from further diluted winery wastes has been proposed. Adsorption onto non ionic polymeric resins and further desorption with ethanolic solutions was studied. Several commercial food grade resins were screened with the aim of selecting the most suited for the practical recovery of phenolic compounds with radical scavenging activity. Under the optimized desorption conditions (using Sepabeads SP207 or Diaion HP20 as adsorbents and eluting with 96% ethanol at 50 °C) a powdered yellow-light brown product with 50% phenolic content, expressed as gallic acid equivalents, was obtained. The radical scavenging capacity of one gram of product was equivalent to 2-3 g of Trolox.

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“…The protein content was determined by the modified Lowry method with bovine serum albumin as the standard (Frolund et al, 1995). Carbohydrate content was measured using the Anthrone method with glucose as the standard (Trevelyan et al, 1952).…”

Section: Extraction Of Eps and Determination Of Eps Componentsmentioning

confidence: 99%

Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

Ruan

Liu

2013

Journal of Environmental Sciences

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Aluminum flocculant can enhance the flocculating performance of activated sludge. However, the binding mechanism of aluminum ion (Al 3+ ) and extracellular polymeric substances (EPS) in activated sludge is unclear due to the complexity of EPS. In this work, threedimensional excitation emission matrix fluorescence spectroscopy (3DEEM), fluorescence quenching titration and Fourier transform infrared spectroscopy (FT-IR) were used to explore the binding behavior and mechanism between Al 3+ and EPS. The results showed that two fluorescence peaks of tyrosine-and tryptophan-like substances were identified in the loosely bound-extracellular polymeric substances (LB-EPS), and three peaks of tyrosine-, tryptophan-and humic-like substances were identified in the tightly boundextracellular polymeric substances (TB-EPS). It was found that these fluorescence peaks could be quenched with Al 3+ at the dosage of 3.0 mg/L, which demonstrated that strong interactions took place between the EPS and Al 3+ . The conditional stability constants for Al 3+ and EPS were determined by the Stern-Volmer equation. As to the binding mechanism, the -OH, N-H, C=O, C-N groups and the sulfur-and phosphorus-containing groups showed complexation action, although the groups in the LB-EPS and TB-EPS showed different behavior. The TB-EPS have stronger binding ability to Al 3+ than the LB-EPS, and TB-EPS play an important role in the interaction with Al 3+ .

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Determination of Yeast Carbohydrates with the Anthrone Reagent

Cited by 142 publications

References 3 publications

A Vaccine Approach for the Prevention of Infections by Multidrug-resistant Enterococcus faecium

A Vaccine Approach for the Prevention of Infections by Multidrug-resistant Enterococcus faecium

Recovery and Concentration of Antioxidants from Winery Wastes

Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

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