Determination of β-lactam antibiotics in milk using micro-flow chemiluminescence system with on-line solid phase extraction

Liu, Wei; Zhang, Zhujun; Zuo-qin, Liu

doi:10.1016/j.aca.2007.04.027

Cited by 54 publications

(41 citation statements)

References 42 publications

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“…Therefore, with the current limitations the use of immunoassays for antibiotics' TDM is not considered as a priority for further development and efforts have been directed to explore other alternatives such as the use of chromatographic techniques. Consequently, liquid-chromatography (LC) coupled to UV [28,[56][57][58][59], chemiluminescence (CL) [60], electrochemical (EC) [61], and mass spectrometry (MS) [62,63], have been widely/successfully used for the detection of β-lactams. Although as compared to mass spectrometry less sophisticated analysis is performed with the use of the other conventional detectors, the speed and sensitivity of the former one surpass those offered by the latter ones (UV, CL, EC).…”

Section: Overview Of Published Analytical Methods Optimized For Theramentioning

confidence: 99%

“…In many cases the use of LC-MS/ MS offers an increase in the sensitivity by several orders of magnitude. For instance, reported lower limit of detection of some β-lactams achieved with LC-UV or LC-EC detectors is 5 mg/L [28,61], while it reached 10-50 times lower levels (0.1-0.5mg/L) when LC-MS/MS is used [64,74]. As in the case of critically ill patients very low levels can be anticipated methods that offer higher sensitivity are preferred.…”

Section: Overview Of Published Analytical Methods Optimized For Theramentioning

confidence: 99%

“…Sample cleanup procedures for β-lactam antibiotics include solid phase extraction [58,60,62,67,72], liquidliquid extraction [59] and protein precipitation using acids [56,61] and organic solvents i.e. : methanol or acetonitrile [64,72,74].…”

Section: Sample Treatmentmentioning

confidence: 99%

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The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

El‐Najjar

Gessner

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Cancer remains a leading cause of mortality and morbidity worldwide. In addition to organ failure, the most frequent reasons for admission of cancer patients to intensive care units (ICU) are: infections and sepsis. As critically ill, the complexity of the health situation of cancer patients renders the standard antimicrobial regimen more complex and even inadequate which results in increased mortality rates. This is due to pathophysiological changes in the volume of distribution, increased clearance, as well as to organ dysfunction. While in the former cases a decrease in drug efficacy is observed, the hallmark of the latter one is overdosing leading to increased toxicity at the expense of efficacy. Furthermore, an additional risk factor is the potential drug-drug interaction between antibiotics and antineoplastic agents. Therefore, therapeutic drug monitoring (TDM) is a necessity to improve the clinical outcome of antimicrobial therapy in cancer patients. To be applied in routine analysis the method used for TDM should be cheap, fast and highly accurate/sensitive. Furthermore, as ICU patients are treated with a cocktail of antibiotics the method has to cover the simultaneous analysis of antibiotics used as a first/second line of treatment. The aim of the current review is to briefly survey the pitfalls in the current antimicrobial therapy and the central role of TDM in dose adjustment and drug-drug interaction's evaluation. A major section is dedicated to summarize the currently published analytical methods and to shed light on the difficulties and potential problems that can be encountered during method development.

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Section: Overview Of Published Analytical Methods Optimized For Theramentioning

confidence: 99%

Section: Overview Of Published Analytical Methods Optimized For Theramentioning

confidence: 99%

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The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

El‐Najjar

Gessner

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Their excessive use in dairy cow diet for the last decades gave rise to stronger bacterial resistance which consequently represents a serious problem in the efficiency of classic anti-bacterial treatment in humans. 1 To face this situation, numerous antibiotics detection protocols have been developed based on conventional chromatographic techniques such as high performance liquid chromatography coupled with UV spectroscopy (HPLC-UV) 2 or mass spectrometry (HPLC-MS). 3 Despite their efficiency and high screening capability, those techniques usually require sample preparation and have to be run by skilled technicians in a laboratory environment.…”

Section: Introductionmentioning

confidence: 99%

Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk

et al. 2009

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A multiplexed immunoassay-based antibiotic sensing device integrated in a lab-on-a-chip format is described. The approach is multidisciplinary and involves the convergent development of a multiantibiotic competitive immunoassay based on sensitive wavelength interrogated optical sensor (WIOS) technology and a polymer-based self-contained microfluidic cartridge. Immunoassay solutions are pressure-driven through external and concerted actuation of a single syringe pump and multiposition valve. Moreover, the use of a novel photosensitive material in a 'one step' fabrication process allowed the rapid fabrication of microfluidic components and interconnection port simultaneously. Pre-filled microfluidic cartridges were used as binary response rapid tests for the simultaneous detection of three antibiotic families -sulfonamides, fluoroquinolones and tetracyclines -in raw milk. For test interpretation, any signal lower than the threshold value obtained for the corresponding Maximum Residue Limit (MRL) concentration (100 mg L À1 ) was considered negative for a given antibiotic. The reliability of the multiplexed detection system was assessed by way of a validation test carried out on a series of six blind milk samples. A test accuracy of 95% was calculated from this experiment. The whole immunoassay procedure is fast (less than 10 minutes) and easy to handle (automated actuation).

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“…In the past two decades trace determinations by CL have markedly increased [13][14][15][16][17][18]. Such methods have recently been suggested for determination of structurally related antibiotics such as amoxicillin, penicillin G, and many other aminopenicillin compounds [19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Determination of penicillin V potassium in pharmaceuticals and spiked human urine by chemiluminescence

2009

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A simple and selective method for penicillin V potassium (PVK) determination by chemiluminescence (CL) was developed. Oxidation of PVK by alkaline hydrogen peroxide produces CL, which is greatly enhanced by N, N-dimethyl formamide (DMF) and N-cetyl-N,N,N-trimethylammonium bromide (CTMAB). Optimum conditions were established using luminometry. There is a linear relationship between the chemiluminescent peak height and the amount of PVK within the range 0.5 -129.5 mg L -1, with a detection limit of 0.2 mg L -1. The coefficient of variation was 1.2% for 40 mg L -1 PVK solution (n = 7). The method is very simple, has high sensitivity and good selectivity, and is usable for process control. It was successfully utilized for the determination of PVK in pharmaceuticals and spiked human urine. Warsaw and Springer-Verlag Berlin Heidelberg. © Versita

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Determination of β-lactam antibiotics in milk using micro-flow chemiluminescence system with on-line solid phase extraction

Cited by 54 publications

References 42 publications

The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

The use of liquid chromatography-tandem mass spectrometry for therapeutic drug monitoring of antibiotics in cancer patients

Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk

Determination of penicillin V potassium in pharmaceuticals and spiked human urine by chemiluminescence

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