Determination strategies of phytohormones: recent advances

Du, Fuyou; Bai, Yu; Liu, Huwei

doi:10.1039/c0ay00471e

Cited by 42 publications

(32 citation statements)

References 95 publications

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“…Chromatographic methods for auxin quantification date from the mid-20th century [99][100][101][102] including early use on forestry trees such as Salix × fragilis L. [103], Picea abies [104], and Populus tremula L. [105]. Chromatographic methods can provide excellent hormone separation and, when coupled with mass spectrometry (MS), allow quantification of compounds from multiple hormone classes in the same sample [69,106]. These methods have greatly improved endogenous auxin identification in forestry species [107][108][109][110].…”

Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

“…These kits allowed the analysis of multiple samples in microtiter plates [89][90][91][92]. Immunoenzymatic techniques are considered to have high specificity for plant hormones, but the long periods required for antibody preparation [69], the cross reactivity of antibodies with multiple compounds from the same hormone class [93][94][95], and the possibility of interference from other compounds [88,96] have been major limitations.…”

Section: Immunoassaysmentioning

confidence: 99%

“…Four consecutive 30-min extractions using ether were also effective for auxin extraction at room temperature [64]. However, bioassay methods gradually became obsolete because of their low specificity for auxin, imprecise results, and the long durations required for bioassay plant preparation [65][66][67][68][69].…”

Section: Introductionmentioning

confidence: 99%

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The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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Qualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees.

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Section: Chromatography and Mass Spectrometrymentioning

confidence: 99%

Section: Immunoassaysmentioning

confidence: 99%

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The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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“…Together with molecular biology approaches, several analytical methods have been developed for the determination of phytohormones in plants, mainly based on high performance liquid chromatography (HPLC) coupled with mass spectrometry (MS or MS/MS) [9]. In MS analysis the use of isotope labeled compounds (expensive and not always available) is mandatory to compensate for ionization suppression caused by co-eluting components in the plant matrix [10].…”

Section: Introductionmentioning

confidence: 99%

“…The commercial sorbents are usually silica and bonded silica such as C8 and C18, which suffer from the non-specific selectivity in isolating plant hormones from plants (e.g. C18 cartridges are capable of retaining lipids and plant pigments in the crude extract) [9].…”

Section: Introductionmentioning

confidence: 99%

New polymeric sorbent for the solid-phase extraction of indole-3-acetic acid from plants followed by liquid chromatography — Fluorescence detector

Campanella

Pulidori

Onor

et al. 2016

Microchemical Journal

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Plant hormones play a crucial role in controlling plant growth and development. These groups of naturally occurring substances trigger physiological processes at very low concentrations, which require sensitive techniques for their quantitation. This study reports on the development of a newly synthesized polymer sorbent for the analysis of indole-3-acetic acid (IAA), which is the most important auxin in plants, from complex raw matrices, such as plant extracts. IAA was determined by high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FD). The polymer developed was able to bind about 94% of IAA with a reproducible manner. The release of IAA in methanol was more than 70% for both of them. Non-specific hydrophobic interactions are, likely, the dominant driving force of the binding between IAA and the polymer. This methodology was applied for the determination of IAA in raw lemon leaves. The detection and quantification limits for IAA in real samples were 0.50 ± 0.08 ng/g and 2.64 ± 0.09 ng/g (n = 3, α = 0.05), respectively. As expected, the IAA concentration in uninfected lemon sample was significantly lower respect to the concentration found in infected sample.

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