Determination the Binding Constant of Interaction of Mobic With Glutathione Using Square Wave Voltammetry

Abstract

Energy in living system can produced through the electron transfer, which necessary to achieve physiological functions of the cells, as a result free radicals of oxygen as well nitrogen species released and caused cell damage due to oxidation stress. Hence, these reactive species involve in the inflammation. This contradictory effect of oxygen necessitated the development of an antioxidant such as Glutathione (Glu.) to protect cell against oxidation by scavenging and inhibiting the action of free radicals. This research studied the electrochemical behavior of Glu. as antioxidant, Mobic as non-sterodial anti-inflammatory drugs( NSAIDs) and their interaction using square wave voltammetry technique (SWV) on the hanging mercury dropping electrode (HMDE) as working electrode, Pt-wire as an auxiliary electrode and Ag/AgCl in Sat. KCl as a reference electrode. Glutathione showed an reduction peak (at -0.305 V) in pH 4 of acetate buffer solution. While, Mobic showed Under the same default Condition a reduction peak at ( -1.15 V). Optimum condition were investigated such as pH, start and end potential, deposition potential, deposition time, equilibrium time, voltage step, amplitude, frequency, the size of the mercury drop and sweep rate. As well, the interaction between the Glu. and Mobic was evaluated by utilizing the calculation of binding constant.