The effect of soil and irrigation water quality in three sites of the Khasah Su River basin Kirkuk-Iraq was studied on the values of total chlorophyll, carbohydrates, and proteins of cress, parsley, celery, and basil plants. Samples were collected from November 2021 to May 2022. Sampling sites in the Khasah Su River located between longitudes 44o 23/ and 44o 24/ and latitudes 35o 24/ and 35o 29/. Soil quality index (SQI) values documented poor soil fertility in all sites, especially on the third site. Irrigation water quality index (IWQI) values were excellent in the first site, good in the second site, and poor in the third site. The basil plant recorded the lowest mean values of carbohydrates 0.59ppm in the first site and proteins 0.26ppm in the second site and the cress plant recorded the lowest mean value of total chlorophyll at 7.92ppm in the first site. Parsley plant recorded the highest mean values of carbohydrates 5.69ppm and total chlorophyll 21.46ppm, and cress plant highest average values of proteins 1.63ppm on the third site. Basil and cress plants were the most affected by the decrease in soil quality and irrigation water index at the study sites, while parsley and celery plants were the least affected.