Determining 1-kestose, nystose and raffinose oligosaccharides in grape juices and wines using HPLC: method validation and characterization of products from Northeast Brazil

Lima, Marcos dos Santos; Nunes, Polyana Campos; Silva, Bernadete de Lourdes de Araújo; Padilha, Carla Valéria da Silva; Bonfim, Thaís Helena Figueiredo do; Stamford, Tânia Lúcia Montenegro; Vasconcelos, Margarida Angélica da Silva; Aquino, Jailane de Souza

doi:10.1007/s13197-019-03936-x

Cited by 22 publications

(5 citation statements)

References 27 publications

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“…But, its concentration in D plants was null or weak at T2 and T3, suggesting its concentration was related to disease symptom expression. 1-kestose is known to be produced by several plants such as Banana ( Agopian et al., 2008 ), Asparagus ( Forsythe et al., 1990 ), and grapevine ( Dos Santos Lima et al., 2019 ), and was also described in human to make the resident bifidobacterial more vigorous in the intestinal flora ( Hidaka et al., 1986 ). Mannitol is a polyol commonly found in plants and fungi.…”

Section: Discussionmentioning

confidence: 99%

Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine

Moret,

Jacquens,

Larignon

et al. 2024

Front. Plant Sci.

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Botryosphaeria dieback is a grapevine trunk disease caused by fungi of the Botryosphaeriaceae family, which attacks more specifically the woody tissues. The infection leads to different symptoms including a severe form with a leaf drop as well as premature plant death. Botryosphaeria dieback causes major economic losses, since no effective treatment is yet available. A better understanding is necessary to find solutions to fight this disease. In this study, our objective was to characterize the “leaf drop” form by (1) looking for the presence of pathogens in the basal internodes of stems, (2) quantifying blocked vessels by tylosis and/or gummosis, and (3) describing the impact of the disease on vine physiology (gene expression and metabolome) and development (establishment and functioning of the cambium and phellogen) at the level of xylem and phloem of basal stem internodes. Our study has shown that Botryosphaeriaceae were present in both phloem and xylem of the basal internodes of the annual stem, with xylem vessels obturated. We have also clearly demonstrated that gene expression and metabolite profiles were strongly modified in both xylem and phloem of diseased plants. Differences in stems between healthy (control, C) and diseased (D) plants were low at flowering (vines not yet symptomatic), higher at the onset of symptom expression and still present, although less marked, at full disease expression. qRT-PCR analysis showed in both phloem and xylem an overexpression of genes involved in plant defense, and a repression of genes related to meristematic activity (i.e. vascular cambium and phellogen). Metabolomic analysis showed specific fingerprints in stems of healthy and diseased plants from the onset of symptom expression, with an increase of the level of phytoalexins and mannitol, and a decrease of 1-kestose one. At the structural level, many alterations were observed in internodes, even before the onset of symptoms: a classical obstruction of xylem vessels and, for the first time, a disorganization of the secondary phloem with an obstruction of the sieve plates by callose. The disease modifies the development of both secondary phloem (liber) and phellogen. Altogether, this study combining different approaches allowed to highlight deep vine dysfunction in the internodes at the base of stems, that may explain vine decline due to Botryosphaeria dieback.

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Section: Discussionmentioning

confidence: 99%

Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine

Moret,

Jacquens,

Larignon

et al. 2024

Front. Plant Sci.

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“…For the column assessment, particularly the resolution of glucose and fructose and their overlays with interferences were compared. Typically, either cationic exchange resins (mainly hydrogen, lead, or sodium form) or amino phases are applied (Dos Santos Lima et al, 2019) and, thus, are evaluated for fructan analysis in stillage (for more information on the column selection see Supporting Information). Columns with calcium (Sangeetha et al, 2005) and silver (Vega & Zuniga‐Hansen, 2015) phases have also been considered but a priori assessed as inappropriate for stillage.…”

Section: Methodsmentioning

confidence: 99%

Quantification of fructans in cereal‐based bioethanol stillage based on a simplified analytical method

Zimmermann

Kaltschmitt

2022

Cereal Chem

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Background and Objectives Cereal‐based bioethanol stillage, the main by‐product of industrial bioethanol production, is a potential substrate for fructans. However, the quantification of fructans in such complex sample matrices is still a challenge for the corresponding analytics to be overcome to allow for the identification and utilization of such unused fructan sources. Especially a possible utilization or rather the corresponding process development requires appropriate analytics first. Thus, this paper aims to develop and apply a method for fructan quantification in bioethanol stillage using common a high‐performance liquid chromatography–refractive index detector. Findings A new approach for fructan quantification is presented allowing for simple fructan determination in bioethanol stillage for comparably high sample throughput. The developed analytical method performs fructan quantification by means of fructose‐ and glucose‐equivalent determination after targeted acidic hydrolysis adapted to the respective sample matrix. On the basis of this method, up to 2.5% of fructans were found in the dry matter of cereal‐based bioethanol stillage. Conclusion Cereal‐based bioethanol stillage is assessed to be a potential source of fructans. The presented analytical method can be the basis for respective process development and, thus, may contribute to a higher‐value utilization of stillage. Significance and Novelty According to current knowledge, no determination of fructans in stillage or corresponding analytical methods have been published so far.

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“…Multiple types of oligosaccharides have been identified in grapes, wine, and wine coproducts in recent studies. Blanch et al [ 26 ] found 5 fructo-oligosaccharides (1-kestose, neokestose, nystose, nystose b, and kestopentaose) in red table grapes, while Dos Santos Lima et al [ 27 ] identified 1-ketose, nystose, and raffinose oligosaccharides in grape juice and wine. Oligosaccharides are present in both red and white wines, as shown in the study conducted by Bordiga et al [ 28 ], who reported 45 distinct oligosaccharides in wine with degrees of polymerization of 3–14 monosaccharide building blocks made of glucose, arabinose, xylose, mannose, rhamnose, fucose, galacturonic acid, and glucuronic acid.…”

Section: Prebiotics In Grape Pomacementioning

confidence: 99%

Uncovering the promising role of grape pomace as a modulator of the gut microbiome: An in-depth review

Sinrod,

Shah,

Surek

et al. 2023

Heliyon

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Determining 1-kestose, nystose and raffinose oligosaccharides in grape juices and wines using HPLC: method validation and characterization of products from Northeast Brazil

Cited by 22 publications

References 27 publications

Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine

Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine

Quantification of fructans in cereal‐based bioethanol stillage based on a simplified analytical method

Uncovering the promising role of grape pomace as a modulator of the gut microbiome: An in-depth review

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