Abstract:qualitative indicators of dynamics and the quantitative methods of filtered fluorescence correlation spectroscopy (fFCS) and dynamic photon distribution analysis (PDA), can be extended and applied to three-color FRET experiments. Specifically, in three-color pulsed interleaved excitation experiments (PIE) with multiparameter fluorescence detection (MFD), the multidimensional information of color, lifetime and anisotropy offers superior contrast to separate different conformational states. This increases the ro… Show more
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