Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface

Abstract

Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to determine suitable concentrations of RB in these bonding agents for imaging in the confocal laser scanning microscope. This study provides systematical strategies for adding RB to viscous dental adhesive resins, focusing on the determination of the lowest range of dye concentrations necessary to achieve an acceptable image of the dentin/adhesive interface. It was demonstrated that optimized images of the resin distribution in dentin can be produced with 0.1-0.02 mg/mL of RB in the (tested) adhesives. Our approaches took into account aspects related to the dye concentration, photophysical parameters in different host media, specimen composition and morphology to develop a rational use of the fluorescent agent with the resin-based materials. Information gained from this work can help optimize labeling methods using dispersions of low-molecular-weight dyes in different monomer blend systems.

Comparative Evaluation of Resin Dentin Interface using Universal and Total- Etch Adhesive Systems on Sound and Eroded Dentin: In Vitro Study

“…22,32,33 To distinguish the resin-dentin interface under confocal microscopy, the universal adhesive agent was incorporated with a concentration of 0.1 mg/mL of Rhodamine B dye before application on the dentin surface, as it easily penetrates into the dentin and act as a contrast agent. 25,34 Depending on the type of group, the restorative procedures were done on all the samples. Resin-dentin interface, has a thin layer of resin-reinforced dentin that binds the two different materials together on a molecular level which is often called as hybrid layer.…”

“…To distinguish the resin-dentin interface under confocal microscopy, the universal adhesive agent was incorporated with a concentration of 0.1 mg/mL of Rhodamine B dye before application on the dentin surface, as it easily penetrates into the dentin and act as a contrast agent. 25 34 …”

“…Rhodamine B dye with 0.01%wt mixed with 5 mL of universal adhesive agent (G-Premio Bond, GC Corporation, Tokyo, Japan) as suggested by Júnior et al was used to obtain an intense fluorescence to aid in the visualization of the bonding agent distribution on the resin-dentin interface. 25 The materials used in this study are specified in Table 1 .…”

Confocal laser scanning microscopic analysis of the penetration of an epoxy resin‐based sealer into dentinal tubules after calcium hydroxide dressing

“…(20) found that rhodamine B dye can influence the physical–mechanical properties of dental adhesive resins, and Bim Júnior et al . (21) observed that the concentration of this dye influences the image labelling in confocal laser scanning microscopy. The present study used a concentration of 0.01%, thus following studies by Russel et al .…”

Dentin bond strength evaluation between a conventional and universal adhesive using etch-and-rinse strategy

“…The remained 30 teeth were prepared as describe above, but the adhesive systems were labeled with rhodamine B (0.02 μg/mL for SU and 0.1 μg/mL for MP) 35 , in order to allow the analysis of the micromorphology of the adhesive interface with greater accuracy.…”