Determining post‐thaw CD34+ cell dose of cryopreserved haematopoietic progenitor cells demonstrates high recovery and confirms their integrity

Abstract

Our data show that most CD34+ cells survive cryopreservation, regardless of the overall post-thaw total nucleated cell viability. Measuring the number of viable CD34 cells post-thaw might be of importance, and in cases of low viability can confirm the quality of the product issued.

Apparent mtDNA sequence heterogeneity in single human blood CD34+ cells is markedly affected by storage and transport

“…As we only cultured CD34 + cells for a week, and this population was functionally heterogeneous, our conditions favored detection of growth of more differentiated lineage-committed progenitors rather than primitive HSCs that require more time in vitro to generate progeny. While we inferred that more quiescent cells with properties closer to a hematopoietic stem cell were preserved in group 1 specimens, as would be suggested by the literature [18, 19], we were not able to directly test for these cells in these samples.…”

“…Since we removed the dead cells by 7-AAD staining during the sorting, we do not think the enriched CD34 + cells in the MNCs of group I were artifacts of non-specific staining of dead cells. The enrichment of CD34 + cells in group I more likely and simply is explained by higher viability of CD34 + cells compared with more mature leucocytes, as others have shown that most hematopoietic progenitor cells, characterized by positive staining for CD34, were more resistant to cryopreservation injury than mature mononucleated cells, regardless of the overall post-thaw total nucleated cell viability [18, 19]. …”

An automatic wash method for dimethyl sulfoxide removal in autologous hematopoietic stem cell transplantation decreases the adverse effects related to infusion

“…Efficient methods for long‐term preservation of enough viable cells for reliable engraftment are required in almost all autologous and in some allogeneic transplants 22‐26 . Freezing is the best available method to preserve cells.…”

Postthaw clotting of peripheral blood stem cell products due to insufficient anticoagulant

“…Products that were collected with addition of heparin (mostly pediatric donors) were excluded from the analysis. All collection procedures were performed with a COBE Spectra continuous-flow blood cell separator (Gambro BCT, Lakewood, CO), and the products were processed, cryopreserved, and thawed as previously described [8]. Briefly, PBSC products were cryopreserved, after removing plasma, at a concentration of 2-3 310 8 total nucleated cells (TNC) per milliliter in a cryopreservation solution containing dimethyl sulfoxide and human serum albumin, and stored in the vapor phase over liquid nitrogen.…”