Determining simple sulfhydryl compounds (low molecular weight) and their contents in biological samples by using 2,2′-dithiobis-(5-nitropyridine)

A Poly(oxyethylene)-Supported Cys–Pro–Leu–Cys/Fe(II) Complex as a Rubredoxin Model: Protection of the Fe–Cys Coordination from Hydrolysis in Aqueous Solution

“…The Cys Cp carbon signal of Cys(Acm) was observed at 40.1 ppm in CDC1 3 , while that of Cys(HgCl) shifted to 31.0 ppm in Me 2 SO-d 6 . Such a shift has also been observed for a 13 C NMR signal of the Cp carbon adjacent to a thiolato ligand coordinated to a Pd(II) ion. 14 A lowfield shift (12 ppm) of Cp signal was induced by Hg-S bonds (Table II) Table II.…”

“…70) solutions was recognized. Therefore, the difference in the chemical shifts of 13 C NMR signals and the absence of any distinction between the CD spectra in MeOH and water are ascribed to the interactions of the side chains, but not to the change in conformational probability about amide chromophores. Thus, the slight conformational change about the chiral Ca of the main chain with a hydrophobic interaction, though local interactions in the case of chains, may leads to considerable change in the chemical shifts assigned to Pro and Leu residues.…”

“…14 A lowfield shift (12 ppm) of Cp signal was induced by Hg-S bonds (Table II) Table II. 13 Table I. The 13 C chemical shifts of the Leu Ca (db= 2.3 ppm) (db is the difference between the chemical shifts in Me 2 SO-d 6 and in D20), Leu Cb (M = 1.3 ppm), and Pro Cy (db= 1.3 ppm) carbons in D 2 0 suggest the occurrence of hydrophobic interactions by Pro and Leu side chains.…”

Application of β-cyclodextrin/MnFe2O4 magnetic nanoparticles as a catalyst for fast chemiluminescence determination of glutathione in human blood using luminol-diperiodatoargentate(III) System

“…Measurement of free GSH in the blood samples is essential to estimate the detoxification and redox status of cells because it has the protective role against oxidative and free radical-mediated radiation cell injury. 3 A number of methods used for the determination of GSH have been reported including enzymatic, 4 spectrophotometric, [5][6][7] high performance liquid chromatography (HPLC), 8,9 electrochemical methods [10][11][12][13][14][15][16][17][18] and chemiluminescence. [19][20][21][22][23] The spectrophotometric methods are simple but not sufficiently sensitive.…”

Spectrophotometric Determination of Aliphatic Thiols Based on Redox Color Reaction with Copper(II) Complex of 1,10-Phenanthroline in Dimethyl Sulfoxide

“…However, no spectrophotometric method for aliphatic thiols has been established, though many spectrophotometric methods have been widely used for such water-soluble thiols as cysteine and glutathione. 4,5 We have previously shown that a Copper(II)-1,10-phenanthroline(phen) complex ion in dimethyl sulfoxide is quantitatively reduced by aliphatic thiols to the intensely colored copper(I)-phen complex ion. 6 Based on this finding, we proposed a spectrophotometric method for the determination of aliphatic thiols.…”