2023
DOI: 10.3390/membranes13020129
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Determining the Bending Rigidity of Free-Standing Planar Phospholipid Bilayers

Abstract: We describe a method to determine membrane bending rigidity from capacitance measurements on large area, free-standing, planar, biomembranes. The bending rigidity of lipid membranes is an important biological mechanical property that is commonly optically measured in vesicles, but difficult to quantify in a planar, unsupported system. To accomplish this, we simultaneously image and apply an electric potential to free-standing, millimeter area, planar lipid bilayers composed of DOPC and DOPG phospholipids to me… Show more

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Cited by 2 publications
(2 citation statements)
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“…Bilayers are formed on the LAMB platform as previously described. [35][36][37][38][39] Briefly, DOPC and DOPE-cb are diluted in chloroform to form stock solutions. These stock solutions are then combined in a 7 mL scintillation vial according to a target molar composition, typically 99 to 1 DOPC to DOPE-cb.…”
Section: Bilayer Fabricationmentioning
confidence: 99%
“…Bilayers are formed on the LAMB platform as previously described. [35][36][37][38][39] Briefly, DOPC and DOPE-cb are diluted in chloroform to form stock solutions. These stock solutions are then combined in a 7 mL scintillation vial according to a target molar composition, typically 99 to 1 DOPC to DOPE-cb.…”
Section: Bilayer Fabricationmentioning
confidence: 99%
“…To begin to understand how lipid chemistry and ionic solution environment dictates the interactions between lipid bilayers, we use a modified thin film balance to create, image, and carefully drain an aqueous phospholipid (PL) thin film in air. , While our previous work loaded oil in the microchip surrounded by an aqueous phase to form a tensionable, free-standing, large area phospholipid bilayer in order to measure membrane Young’s modulus and bending rigidity in symmetric, and asymmetric environments, , here we create inverted phospholipid bilayers in water in order to examine headgroup-headgroup interactions . We suspend the lipids in an aqueous solution and create a thick film (that is subsequently thinned) surrounded by air.…”
Section: Introductionmentioning
confidence: 99%