Structural health monitoring plays a crucial role in ensuring the integrity and safety of engineering structures such as steel beams. This research paper presents a comprehensive methodology for detecting transverse cracks in beams with a constant section and any boundary conditions. The proposed approach utilizes the normalized squared modal curvature of the beam, the damage severity, and the natural frequency of the undamaged beam. By analyzing the natural frequencies of both the undamaged and damaged states, Relative Frequency Shift (RFS) values are obtained. Subsequently, the Damage Location Coefficients (DLC) are calculated by normalizing the RFS values. These DLC values are then employed to establish a comprehensive database of known damage signatures, enabling the training of an artificial neural network (ANN) in MATLAB. The trained ANN can predict the locations of damages for new scenarios by utilizing DLC values obtained from measurements. To validate the effectiveness of the ANN, extensive simulations using Finite Element Method (FEM) and experimental measurements are conducted on a steel cantilever beam. The results demonstrate the ANN’s capability to accurately predict the locations of transverse cracks, showcasing its potential as a reliable tool for structural health monitoring of steel beams.