Flexo printing or flexography is the most common letterpress printing technique today. Compared to other printing techniques, the advantages of flexographic printing are higher printing speed, low material losses, the possibility of using water-based colors, high color consistency on the print itself. The appearance of the final product largely depends on the selection of the anilox roller, i.e. its line and volume, and on the substrate. The research carried out in this paper determined the influence of the anilox roller when printing on different polymer substrates, and colorimetric differences in color deviation were determined in prints that included four different manufacturers of solvent-based paints on five different polymer substrates (transparent BOPP, white LDPE, black and white recycled LDPE, blue LDPE and green LDPE) with anilox rollers of different lines (lpi) and volume (BCM). In the paper there were used three different anilox rollers with 100 lpi, 165 lpi and 220 lpi.