Determining the tranquilization dose and residue of tricaine methanesulfonate (MS-222) in sea bass Lates calcarifer tissue

Xue, Yi-Jing; Chang, Chih‐Cheng; Lai, Jyh-Mirn; Wang, Jiann Hsiung

doi:10.1007/s12562-017-1091-3

Cited by 11 publications

(11 citation statements)

References 26 publications

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“…This has been previously described in rats (Marier et al, 2002; Parke et al, 1974) as being responsible for prolonging the metabolic load on the liver resulting in delayed elimination of monoterpene compounds. By way of comparison, MS‐222 is eliminated most rapidly by a one‐compartment open model with first‐order elimination with an elimination half‐life of 1.3 min (Kiessling et al, 2009), while tissue half‐life can reach 7 h (Xue et al, 2017). Yet, similar serum concentration–time profiles have been described for MS‐222 and eugenol (Rairat et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

A meta‐analytic review of monoterpene for fish anaesthesia

et al. 2023

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The use of anaesthetic agents has been increasing to address the welfare needs of different fish species in scientific and aquaculture practices. MS‐222 is the most used synthetic anaesthetic but some limitations and side effects have been reported. Natural substances have been used as potential substitutes with clove oil playing an important role due to its eugenol content. Yet, other monoterpenes have shown anaesthetic properties in different fish species. As such, a quantitative assessment has been conducted to explicitly relate and summarise the use of monoterpenes with described anaesthetic properties in fish. A combined PubMed, Web of Science, Scopus, PubMed Central and Europe PMC electronic database search was performed, from January 2002 to August 2022, following the PRISMA guidelines. Out of 1555 articles retrieved from the literature search, 30 English reports met the criteria for inclusion in the meta‐analysis through a generic inverse‐variance method (random‐effects model) and according to the fish age. The findings show a total of 10 different monoterpenes with high homogeneity and effectiveness in inducing anaesthesia and recovery according to the fish anaesthetic criteria. Further subgroup analysis showed these effects were independent of fish age. Meta‐regression of the included studies revealed an inverse linear association between anaesthesia induction and recovery time and water bath temperature while no further correlation was found with other confounding factors (body weight, water pH and dissolved oxygen levels). Although further studies will be required, this meta‐analysis provides robust evidence that different monoterpenes serve as an attractive and effective alternative for fish anaesthesia although water bath temperature also played an important role in anaesthesia outcome.

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Section: Discussionmentioning

confidence: 99%

A meta‐analytic review of monoterpene for fish anaesthesia

et al. 2023

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“…Blood samples were collected from five fish in each group at the 2nd and 6th DPI after anaesthesia to avoid the elevation in plasma cortisol level during fish handling; tricaine methane sulfonate (Tricane‐S) was used at a dose of 60 mg L −1 (Xue et al., 2017). One ml of blood was collected via caudal venipuncture from each fish and then placed in a heparinized tube for separation.…”

Section: Methodsmentioning

confidence: 99%

“…was used at a dose of 60 mg L −1 (Xue et al, 2017). One ml of blood was collected via caudal venipuncture from each fish and then placed in a heparinized tube for separation.…”

Section: Plasma Cortisolmentioning

confidence: 99%

The role of Aeromonas genotyping in virulence for Dicentrarchus labrax

Aboyadak,

Soliman,

Nageeb

et al. 2023

Journal of Fish Diseases

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Aeromonas septicemia still represents a serious challenge facing the global aquaculture sector. In the present study, Aeromonas caviae and A. veronii were isolated from four diseased European seabass (Dicentrarchus labrax) farms experiencing a high mortality rate. Diseased fish showed haemorrhages on the external body surface with exophthalmia, cataracts, scale desquamation, skin ulcers and fin erosions. The most common post‐mortem findings were congested internal organs, particularly the liver and posterior kidney. Twenty‐eight A. Veronii and 11 A. caviae isolates were identified biochemically by the Vitek 2 system and then confirmed by PCR and phylogenetic analysis. Hemolysin (hlyA) and aerolysin (aer) were the most abundant virulence genes in the recovered isolates, followed by cytotoxic enterotoxin (act) and heat‐stable enterotoxin (ast). A. caviae was more virulent than A. veronii for D. labrax fingerlings as LD50 ranging between (>1 × 108–6.2 × 107) for A. veronii and (2.9 × 107–8.3 × 107) for A. caviae. The sensitivity test indicated the effectiveness of norfloxacin, doxycycline and oxytetracycline against the tested isolates. Serum cortisol significantly increased in the infected groups, while catalase and glutathione peroxidase activities significantly decreased at 2 days post‐infection (DPI) and then increased at 6 DPI. The presence of virulence genes was associated with bacterial pathogenicity expressed in fish mortality rate. Virulence genes also drastically affect cortisol levels more than catalase and glutathione peroxidase levels.

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“…Xue determined the MS-222 residues in anaesthetized fish. It was found that the half-life period of elimination in muscle and liver were 5.54-5.27h (30 mg/L) and 8.72-7.15h (60 mg/L) respectively [16]. When the concentration was 30 mg/L, the withdrawal period was at least 4.5d; if the concentration was 60 mg/L, the withdrawal period was at least 7.5day.…”

Section: Tissue Residues and Safety Of Ms-222mentioning

confidence: 98%

Research on the Application of Fishery Anesthetic MS-222 and Eugenol in China

2019

International Journal of Innovative Studies in Aquatic Biology

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As a large aquaculture industry country, China has been producing the world's largest number of aquaculture production for many years. In the operation to aquatic animals such as fishing, transportation, artificial propagation, individual marking, internal anatomy and external morphological measurement, etc., aquatic animals will produce strong stress reactions, such as mucus secretion, jumping and struggling, which seriously hamper the smooth development of related work and cause some harm to fish. The scientific and rational use of anesthetics can significantly reduce the stress response of fish, reduce the damage to fish, and facilitate manual operation. At present, there are many kinds of anesthetics for aquatic animals. There are more than 30 kinds, such as eugenol, MS-222, benzocaine, 2-phenoxyethanol, etc. Among them, MS-222 and eugenol are the most widely used in breeding and production. MS-222 has been approved by the Food and Drug Administration (FDA) of the United States for its advantages of safe and convenient use, fast anaesthesia and recovery. It is the most widely used, safe and effective aquatic anaesthetic at present. MS-222 has been reported as an anesthetic for many kinds of fish [1][2][3][4]. Eugenol is a plant extract. It is cheap and easy to get, and the metabolites can be quickly discharged. Eugenol will not induce mutations in the body. It does not cause harm to human beings and the environment. It can be added directly in food. Research of eugenol, as a fish anesthetic of aquaculture animals such as IetalurusPunetaus, has been reported [5][6]. The writer summarizes the anesthetic MS222 and eugenol from the principles of anesthesia and its influencing factors and the current application in production. ANESTHETIC PRINCIPLES AND INFLUENCING FACTORS OF FISHERY ANESTHETICS 2.1. Anesthetic PrinciplesThe action principle of anesthetics: anesthetics first inhibit the cerebral cortex (tactile loss period), then act on the basal ganglia and cerebellum (excitatory period), and finally act on the spinal cord (anaesthesia period). Too much or too long exposure can make the anesthetics deep into the medulla, causing respiration and blood vessel shrink, and central paralysis, and eventually lead to death [7]. Influence Factor Fish Species, Specifications and AgesDifferent breeds react differently to different anesthetics and concentrations, while MS-222 has no effect on the crayfish (Procambarusclarkii). Generally, for the same variety with different specifications, the

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Determining the tranquilization dose and residue of tricaine methanesulfonate (MS-222) in sea bass Lates calcarifer tissue

Cited by 11 publications

References 26 publications

A meta‐analytic review of monoterpene for fish anaesthesia

A meta‐analytic review of monoterpene for fish anaesthesia

The role of Aeromonas genotyping in virulence for Dicentrarchus labrax

Research on the Application of Fishery Anesthetic MS-222 and Eugenol in China

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