Determining tissue conductivity in tissue ablation by nanosecond pulsed electric fields

Oshin, Edwin; Guo, Siqi; Jiang, Chunqi

doi:10.1016/j.bioelechem.2021.107949

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…The increase in tissue conductivity following PEF exposure is consistent with that of many biological tissues reported in the literature [15,16,28,[32][33][34][35], although this is the first report of the effects of PEF exposure on AF cartilage to our knowledge. The AF tissue's σ eq increases with more intense PEF exposures, especially with higher n, and total SED.…”

Section: Discussionsupporting

confidence: 89%

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Electrical impedance decreases in annulus fibrosus cartilage exposed to microsecond pulsed electric fields ex vivo

Atsu

Thompson

2023

Biomed. Phys. Eng. Express

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Electropermeabilization of biomembranes often is measured by microscopic imaging of a membrane-impermeable fluorophore that penetrates the cells following pulsed electric field (PEF) exposure. PEF exposure subsequently changes physiological properties of tissue. One way to probe these changes in tissue is measuring electrical properties by way of electrochemical impedance spectroscopy (EIS). In this study, we analyze impedance and conductivity of bovine annulus fibrosus (AF) cartilage before and after exposures to PEF of 100 µs duration. Two PEF parameters – electric field amplitude and number of pulses – are varied, and total specific dose of PEF is calculated. AF tissue conductivity increases with both amplitude and number of pulses, indicating electropermeabilization of the annulus fibrosus cells. A Live/Dead cell imaging assay validates the EIS measurements, indicating intratissue cell permeabilization by µsPEF exposure. These results support the extension of EIS to monitor extent of electropermeabilization of cells within cartilage tissue.

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Section: Discussionsupporting

confidence: 89%

“…Bioimpedance and electrical conductivity are intrinsic properties related to fluid volume, ion concentration, and membrane permeability. Tissue bioimpedance changes upon PEF-induced electropermeabilization of cell membranes [15][16][17]. Therefore, EIS can be used to quantify electropermeabilization within tissues.…”

Section: Introductionmentioning

confidence: 99%

Electrical impedance decreases in annulus fibrosus cartilage exposed to microsecond pulsed electric fields ex vivo

Atsu

Thompson

2023

Biomed. Phys. Eng. Express

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“…It has been shown that the electrical conductivity of the tissue increases irreversibly during electroporation and that this increase is related to the amplitude and number of pulses 37 . It has also been shown that there is a limit to the effectiveness of the number of pulses, and that conductivity will not increase further when the number of pulses is above a certain threshold 38 . In this study, a high number of pulses was used, so the conductivity σ(r) was simplified to a constant.…”

Section: Resultsmentioning

confidence: 99%

Electroacoustic tomography for real-time visualization of electrical field dynamics in deep tissue during electroporation

Xu,

Sun,

Wang

et al. 2023

Commun Eng

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Despite the widespread applications of electroporation in biotechnology and medicine, monitoring the distribution of deep tissue electrical fields in real-time during treatment continues to pose a challenge. Current medical imaging modalities are unable to monitor electroporation during pulse delivery. Here we propose a method to use electroacoustic tomography (EAT) to prompt the emission of broadband ultrasound waves via electrical energy deposition. EAT boasts submillimeter resolution at depths reaching 7.5 centimeters and can deliver imaging speeds up to 100 frames per second when paired with an ultrasound array system. We’ve successfully detected EAT signals at electric field strengths ranging from 60 volts per centimeter to several tens of kilovolts per centimeter. This establishes EAT as a potential label-free, high-resolution approach for real-time evaluation of deep tissue electroporation during therapeutic procedures.

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Simulation and Experimental Study on Damage of Subcellular Structure by High Voltage and Extremely Short Pulse Electric Field

Ma,

Wang,

Chen

et al. 2023

Lecture Notes in Electrical Engineering

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Determining tissue conductivity in tissue ablation by nanosecond pulsed electric fields

Cited by 6 publications

References 34 publications

Electrical impedance decreases in annulus fibrosus cartilage exposed to microsecond pulsed electric fields ex vivo

Electrical impedance decreases in annulus fibrosus cartilage exposed to microsecond pulsed electric fields ex vivo

Electroacoustic tomography for real-time visualization of electrical field dynamics in deep tissue during electroporation

Simulation and Experimental Study on Damage of Subcellular Structure by High Voltage and Extremely Short Pulse Electric Field

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