Determining two-photon absorption cross sections via nonresonant multiphoton photoacoustic spectroscopy

Kiser, John B.; Chandrasekharan, Nirmala; Cullum, Brian M.

doi:10.1117/12.730458

Cited by 1 publication

(3 citation statements)

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“…[3,4] In one study, Kiser et al have shown that rapid and real-time diagnosis of brain tumors can be performed using ratiometric analysis of spectra obtained from multiphoton photoacoustic spectroscopy. [4] Using flash frozen matched tissue pair samples from multiple individuals and ratiometric analysis of spectra at three different wavelengths in the optical diagnostic window, brain tumors were identified with 99% accuracy. Although the study focused on surface detection of tumor and healthy brain tissues, multiphoton photoacoustic spectroscopy has a huge potential to margin tumors centimeters deep into the tissue.…”

Section: Introductionmentioning

confidence: 99%

“…[2][3][4] Previous studies have demonstrated the potential of this technique for effectively distinguishing between excised healthy and malignant brain tissue samples. [3,4] In one study, Kiser et al have shown that rapid and real-time diagnosis of brain tumors can be performed using ratiometric analysis of spectra obtained from multiphoton photoacoustic spectroscopy. [4] Using flash frozen matched tissue pair samples from multiple individuals and ratiometric analysis of spectra at three different wavelengths in the optical diagnostic window, brain tumors were identified with 99% accuracy.…”

Section: Introductionmentioning

confidence: 99%

“…[2,5,6] However, using multiphoton excitation, near-IR light in the optical diagnostic window can be used to excite chemicals deep into the tissues while the ultrasonic/acoustic signals generated are minimally attenuated before reaching the detector. [2,4,7,8] This paper characterizes NMPPAS by investigating how deep can light penetrate into the tissue and still produce measurable acoustic signals following multiphoton excitation. Additionally, this work also determines the lateral resolution of NMPPAS.…”

Section: Introductionmentioning

confidence: 99%

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Depth and resolution characterization of two-photon photoacoustic spectroscopy for noninvasive subsurface chemical diagnostics

2011

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Photoacoustic spectroscopy is a powerful optical biopsy technique that enables rapid tumor diagnosis in situ. It has also been reported that photoacoustic spectroscopy can be used to diagnose pre-malignant tissue based on the chemical differences between healthy and pre-malignant tissues. Since the acoustic signals obtained from tissues in these analyses suffer from minimum damping, photoacoustic spectroscopy can be highly sensitive. This paper focuses on the characterization of a novel multiphoton excited photoacoustic methodology for margining of malignant and premalignant tissues.The two-photon excitation process in tissues using nanosecond laser pulses produces ultrasonic signals that transmit through tissue with minimal attenuation. Additionally, the two-photon excitation process is highly localized since only ballistic photons contribute to the excitation process; thereby eliminating potential absorption events in tissue not of interest (i.e., along the beam path) and increasing the spatial resolution of the diagnostic technique to that achievable via optics. This work characterizes the two-photon excitation process for photoacoustic signal measurements on a model dye. Using gelatin phantoms to mimic real tissues, tissue penetration studies were performed, revealing chemical species as deep as 1.3 cm in the tissue can easily be detected using this methodology. Furthermore, the resolution of this multiphoton excitation process was determined to be as great as 50 µm (near cellular level resolution).

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Section: Introductionmentioning

confidence: 99%