Deterministic lateral displacement (DLD) in the high Reynolds number regime: high-throughput and dynamic separation characteristics

Dincau, Brian; Aghilinejad, Arian; Hammersley, Taylor; Chen, Xiaolin; Kim, Jong Hoon

doi:10.1007/s10404-018-2078-9

Cited by 48 publications

(59 citation statements)

References 22 publications

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“…that utilizing asymmetrical gaps for DLD device enhanced the separation of red blood cells without restricting the throughput significantly . However, DLD faces major design challenges such as inability to sort cells with similar size and unpredictability of the device performance at high throughputs due to flow‐induced cell deformation . There have been prior attempts to couple DLD and DEP although none of them investigated the applicability of such concept for continuous CTC separation.…”

Section: Introductionmentioning

confidence: 99%

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

et al. 2019

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Detection and analysis of circulating tumor cells (CTCs) have emerged as a promising way to diagnose cancer, study its cellular mechanism, and test or develop potential treatments. However, the rarity of CTCs among peripheral blood cells is a big challenge toward CTC detection. In addition, in cases where there is similar size range between certain types of CTCs (e.g. breast cancer cells) and white blood cells (WBCs), high‐resolution techniques are needed. In the present work, we propose a deterministic dielectrophoresis (DEP) method that combines the concept of deterministic lateral displacement (DLD) and insulator‐based dielectrophoresis (iDEP) techniques that rely on physical markers such as size and dielectric properties to differentiate different type of cells. The proposed deterministic DEP technology takes advantage of frequency‐controlled AC electric field for continuous separation of CTCs from peripheral blood cells. Utilizing numerical modeling, different aspects of coupled DLD‐DEP design such as the required applied voltages, velocities, and geometrical parameters of DLD arrays of microposts are investigated. Regarding the inevitable difference and uncertainty ranges for the reported crossover frequencies of cells, a comprehensive analysis is conducted on applied electric field frequency as design's determinant factor. Deterministic DEP design provides continuous sorting of CTCs from WBCs even with similar size and has the future potential for high throughput and efficiency.

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Section: Introductionmentioning

confidence: 99%

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

et al. 2019

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“…The vortices increase the hydrodynamic radius of the posts reducing the critical radius of the particles. Recently, Dincau et al reported on the numerical simulation and experimental evaluation of the performance of the DLD device operating in the high-Re mode (10 < Re < 60) [326]. They figured out that the vortices appear around Re = 25 and are fully developed under Re = 50, reaching the half-size of the post.…”

Section: Deterministic Lateral Displacementmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…Different sizes of particles have different equilibrium positions in the inertial channel, but the separation efficiency is relatively low. DLD technology achieves separation by designing micro-pillar arrays of different sizes and shapes in microchannels, which has unique advantages in terms of processing speed, throughput, label-free and convenience for integration [18,19,20,21,22]. It is promising for applications in microalgae separation.…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Prototype System towards Microalgae Cell Separation, Treatment and Viability Characterization

Wang

Zhou

et al. 2019

Sensors

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There are a huge number, and abundant types, of microalgae in the ocean; and most of them have various values in many fields, such as food, medicine, energy, feed, etc. Therefore, how to identify and separation of microalgae cells quickly and effectively is a prerequisite for the microalgae research and utilization. Herein, we propose a microfluidic system that comprised microalgae cell separation, treatment and viability characterization. Specifically, the microfluidic separation function is based on the principle of deterministic lateral displacement (DLD), which can separate various microalgae species rapidly by their different sizes. Moreover, a concentration gradient generator is designed in this system to automatically produce gradient concentrations of chemical reagents to optimize the chemical treatment of samples. Finally, a single photon counter was used to evaluate the viability of treated microalgae based on laser-induced fluorescence from the intracellular chlorophyll of microalgae. To the best of our knowledge, this is the first laboratory prototype system combining DLD separation, concentration gradient generator and chlorophyll fluorescence detection technology for fast analysis and treatment of microalgae using marine samples. This study may inspire other novel applications of micro-analytical devices for utilization of microalgae resources, marine ecological environment protection and ship ballast water management.

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Deterministic lateral displacement (DLD) in the high Reynolds number regime: high-throughput and dynamic separation characteristics

Cited by 48 publications

References 22 publications

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

On the design of deterministic dielectrophoresis for continuous separation of circulating tumor cells from peripheral blood cells

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

A Microfluidic Prototype System towards Microalgae Cell Separation, Treatment and Viability Characterization

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