Detonation nanodiamond toxicity in human airway epithelial cells is modulated by air oxidation

Silbajoris, Robert; Linak, William P.; Shenderova, Olga; Winterrowd, Christopher K.; Chang, Huan‐Cheng; Zweíer, Jay L.; Kota, Anirudh; Dailey, Lisa A.; Nunn, Nicholas; Bromberg, Philip A.; Samet, James M.

doi:10.1016/j.diamond.2015.05.007

Cited by 12 publications

(5 citation statements)

References 17 publications

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“… 29 , 75 – 77 Our work on HPHT diamond aligns with studies on detonation diamond despite the compositional differences, 41 with graphitic nanodiamonds suppressing cell proliferation and oxidized nanodiamonds having the lowest cytotoxic effect. 15 , 28 Although we did not observe any anti-proliferative effect of oxidized nanodiamonds, we exposed our cells to a concentration range suitable for experiments interrogating local organelle tracking or temperature sensing, rather than those used for drug delivery, which would be substantially higher. At higher concentrations such as 1 μg mL –1 effects including apoptosis have been observed even with oxidized nanodiamonds.…”

Section: Discussionmentioning

confidence: 89%

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Graphitic and oxidised high pressure high temperature (HPHT) nanodiamonds induce differential biological responses in breast cancer cell lines

et al. 2018

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Section: Discussionmentioning

confidence: 89%

“…Also, as expected, the spectrum exhibited a shift in the C O groups from aldehyde (1721 cm –1 ) to carboxyl (1784 cm –1 ) as the groups were converted into the most oxidized form through the heating process. 15 , 33 , 46 – 50 …”

Section: Resultsmentioning

confidence: 99%

Graphitic and oxidised high pressure high temperature (HPHT) nanodiamonds induce differential biological responses in breast cancer cell lines

et al. 2018

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“…FNDs have been proven to be suitable for long-term in vivo imaging in Caenorhabditis elegans, without causing toxicity [21][22] ; in cancer and stem cells, division and differentiation are not affected by 100 nm NDs 23 . In a different experimental model, airway epithelial cells, functionalization may reduce ND inflammogenicity 24 , while a remarkable dose-dependent decrease of neurite length of central and peripheral cultured neurons after FNDs seeding has been demonstrated 25 .…”

Section: Introductionmentioning

confidence: 99%

Nanodiamonds-induced effects on neuronal firing of mouse hippocampal microcircuits

Guarina

Calorio

Gavello

et al. 2018

Sci Rep

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Fluorescent nanodiamonds (FND) are carbon-based nanomaterials that can efficiently incorporate optically active photoluminescent centers such as the nitrogen-vacancy complex, thus making them promising candidates as optical biolabels and drug-delivery agents. FNDs exhibit bright fluorescence without photobleaching combined with high uptake rate and low cytotoxicity. Focusing on FNDs interference with neuronal function, here we examined their effect on cultured hippocampal neurons, monitoring the whole network development as well as the electrophysiological properties of single neurons. We observed that FNDs drastically decreased the frequency of inhibitory (from 1.81 Hz to 0.86 Hz) and excitatory (from 1.61 to 0.68 Hz) miniature postsynaptic currents, and consistently reduced action potential (AP) firing frequency (by 36%), as measured by microelectrode arrays. On the contrary, bursts synchronization was preserved, as well as the amplitude of spontaneous inhibitory and excitatory events. Current-clamp recordings revealed that the ratio of neurons responding with AP trains of high-frequency (fast-spiking) versus neurons responding with trains of low-frequency (slow-spiking) was unaltered, suggesting that FNDs exerted a comparable action on neuronal subpopulations. At the single cell level, rapid onset of the somatic AP (“kink”) was drastically reduced in FND-treated neurons, suggesting a reduced contribution of axonal and dendritic components while preserving neuronal excitability.

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“…Silbajoris et al demonstrated that despite being non-cytotoxic to the human airway epithelial cells (HAEC) in vitro , the DND induced inflammatory gene expression in a dose-dependent manner ( Silbajoris et al, 2009 ). Further research revealed that although the mechanism involved is dependent on the production of intracellular H 2 O 2 , suggesting that DND exposure presents oxidative stress to HAEC, further oxidation of the DND surface can reduce this effect ( Silbajoris et al, 2015 ). The loss of non-diamond carbon from the DND surface correlated to a reduction in the inflammogenic activity, which was observed for the air-annealed samples following heating periods even as short as 5 min.…”

Section: Introductionmentioning

confidence: 99%

Plasma & Microwaves as Greener Options for Nanodiamond Purification: Insight Into Cytocompatibility

Mitev

Alsharabasy

Morrison

et al. 2021

Front. Bioeng. Biotechnol.

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The potential biomedical applications of nanodiamond have been considered over the last few decades. However, there is still uncertainty regarding the extent to which the surface characteristics of this material can influence potential applications. The present study investigated the effects of surface characteristics alongside the prospective of improving nanodiamond production using cold plasma and microwave technologies for the surface tailoring of the nanocarbons. Numerous approaches were applied to purify, refine and modify a group of nanosized diamonds at each step of their production cycle: from the detonation soot as the initial raw material to already certified samples. The degree of surface changes were deliberately performed slowly and kept at different non-diamond carbon presence stages, non-carbon elemental content, and amount converted superficial moieties. In total, 21 treatment procedures and 35 types of nanosize diamond products were investigated. In addition cultures of human fibroblast cells showed enhanced viability in the presence of many of the processed nanodiamonds, indicating the potential for dermal applications of these remarkable nanomaterials.

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Detonation nanodiamond toxicity in human airway epithelial cells is modulated by air oxidation

Cited by 12 publications

References 17 publications

Graphitic and oxidised high pressure high temperature (HPHT) nanodiamonds induce differential biological responses in breast cancer cell lines

Graphitic and oxidised high pressure high temperature (HPHT) nanodiamonds induce differential biological responses in breast cancer cell lines

Nanodiamonds-induced effects on neuronal firing of mouse hippocampal microcircuits

Plasma & Microwaves as Greener Options for Nanodiamond Purification: Insight Into Cytocompatibility

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