Detoxification of biomass hydrolysates with nucleophilic amino acids enhances alcoholic fermentation

Xie, Rui; Tu, Maobing; Carvin, Jamarius; Wu, Yufeng

doi:10.1016/j.biortech.2015.03.030

Cited by 16 publications

(15 citation statements)

References 30 publications

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“…Besides, the ratio between holocellulose and lignin is important in some conversion processes. For example, for obtaining ethanol through bio-chemical processing, a biomass with a high content of holocellulose is preferred (Xie et al, 2015) (lignin has very low biodegradability properties). However, pyrolysis of biomass with a high percentage of lignin can produce better bio-char yields (Yang et al, 2006).…”

Section: Determination Of Content In Holocelluloses and Ligninmentioning

confidence: 99%

Physico-chemical characterization of pine cone shell and its use as biosorbent and fuel

Almendros

Martín-Lara

Ronda

et al. 2015

Bioresource Technology

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Section: Determination Of Content In Holocelluloses and Ligninmentioning

confidence: 99%

Physico-chemical characterization of pine cone shell and its use as biosorbent and fuel

Almendros

Martín-Lara

Ronda

et al. 2015

Bioresource Technology

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“…Klinke et al reported 26 degradation products resulted from alkaline treated wheat straw hydrolysates, including acids, furans and phenols [ 13 ]. Although significant efforts have been made to identify the potential inhibitors on microbial fermentation, no single compound has been determined to be the dominant inhibitor [ 15 ]. Furfural and HMF content has been suggested to be the important indicator of prehydrolysate toxicity, but they are not the major inhibitors [ 4 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates

Zhang

Xia

et al. 2018

Biotechnol Biofuels

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BackgroundBiomass prehydrolysates from dilute acid pretreatment contain a considerable amount of fermentable sugars for biofuels production. However, carbonyl degradation compounds present severe toxicity to fermentation microbes. Furans (such as furfural and hydroxymethylfurfural), aliphatic acids (such as acetic acid, formic acid and levulinic acid) and phenolic compounds (such as vanillin and syringaldehyde) have been suggested to be the main inhibitors in biomass prehydrolysates. However, no single compound has been determined as the dominant toxic inhibitor. The effects of various detoxification methods on inhibitors removal have not been fully understood.ResultsThe effects of overliming and activated carbon (AC) detoxification on the removal of inhibitors and butanol fermentation of the poplar prehydrolysates were investigated. Gas chromatography–mass spectrometry (GC/MS) was used to identify and quantify 46 carbonyl compounds as potential inhibitors. It was observed that overliming and AC treatment alone did not make the prehydrolysates fermentable with Clostridium saccharobutylicum. The sequential overliming and AC resulted in a remarkable fermentability and a high butanol yield at 0.22 g g−1 sugar. The inhibitor removal in the prehydrolysates treated by overliming and AC was also examined by GC/MS. Overliming removed 75.6% of furan derivatives and 68.1% of aromatic monomers. In comparison, AC (5.0% w/v) removed 77.9% of furan derivatives and 98.6% of aromatic monomers. In addition, overliming removed much more 2,5-furandicarboxyaldehyde, 5-ethylfuran-2-carbaldehyde and 2,5-hexanedione than AC did. On the contrary, AC could remove considerably more phenolic acids than overliming. In the sequential detoxification, both dialdehydes/diketones and phenolic acids were extensively removed. This could be the main reason why the sequential detoxification enabled a remarkable ABE fermentation for the prehydrolysates.ConclusionsThis study indicated that the effect of overliming and AC treatment on inhibitors removal was related to their chemical structures. Overliming removed more dialdehydes and diketones than AC treatment, while AC removed more phenolic acids than overliming. Sequential overliming and AC treatment were required to make the prehydrolysates fermentable with C. saccharobutylicum. The study also suggested different detoxification method was needed for ABE fermentation of the prehydrolysate as compared to ethanol fermentation.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1182-0) contains supplementary material, which is available to authorized users.

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“…The reaction products of cysteine and inhibitor showed no negative side effects on the fermentation. Additionally, the amino acid treatment is easy to apply, although the viability of the treatment for industrial processes is still unclear [42].…”

Section: Chemical Degradation Of Inhibitorsmentioning

confidence: 99%

Origin, Impact and Control of Lignocellulosic Inhibitors in Bioethanol Production—A Review

Sjulander

Kikas

2020

Energies

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Bioethanol production from lignocellulosic biomass is still struggling with many obstacles. One of them is lignocellulosic inhibitors. The aim of this review is to discuss the most known inhibitors. Additionally, the review addresses different detoxification methods to degrade or to remove inhibitors from lignocellulosic hydrolysates. Inhibitors are formed during the pretreatment of biomass. They derive from the structural polymers-cellulose, hemicellulose and lignin. The formation of inhibitors depends on the pretreatment conditions. Inhibitors can have a negative influence on both the enzymatic hydrolysis and fermentation of lignocellulosic hydrolysates. The inhibition mechanisms can be, for example, deactivation of enzymes or impairment of vital cell structures. The toxicity of each inhibitor depends on its chemical and physical properties. To decrease the negative effects of inhibitors, different detoxification methods have been researched. Those methods focus on the chemical modification of inhibitors into less toxic forms or on the separation of inhibitors from lignocellulosic hydrolysates. Each detoxification method has its limitations on the removal of certain inhibitors. To choose a suitable detoxification method, a deep molecular understanding of the inhibition mechanism and the inhibitor formation is necessary.

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Detoxification of biomass hydrolysates with nucleophilic amino acids enhances alcoholic fermentation

Cited by 16 publications

References 30 publications

Physico-chemical characterization of pine cone shell and its use as biosorbent and fuel

Physico-chemical characterization of pine cone shell and its use as biosorbent and fuel

Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates

Origin, Impact and Control of Lignocellulosic Inhibitors in Bioethanol Production—A Review

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