Detoxification of corn stover prehydrolyzate by trialkylamine extraction to improve the ethanol production with Pichia stipitis CBS 5776

Zhu, Jianzhong; Yong, Qiang; Xu, Yong; Yu, Shiyuan

doi:10.1016/j.biortech.2010.09.083

Cited by 64 publications

(50 citation statements)

References 25 publications

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“…Trialkylamine [79] Liquid-solid extraction Activated carbon [80] Ion exchange [38,81] Lignin [82] Microbial treatment Coniochaeta ligniaria [83,84] Trichoderma reesei [33,85] Ureibacillus thermosphaericus [86] a The phenolics [89,90], furan aldehydes [91,92], and aliphatic acids [93,94]. Furthermore, overexpression of a transcription factor, Yap1 [95], and of multidrug-resistance proteins [95] has also generated hyperresistant S. cerevisiae transformants.…”

Section: Strategies To Counteract Inhibition Problemsmentioning

confidence: 99%

- Synthetic Biology: A Promising Technology for Biofuel Production

2015

New Biotechnologies for Increased Energy Security

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Section: Strategies To Counteract Inhibition Problemsmentioning

confidence: 99%

- Synthetic Biology: A Promising Technology for Biofuel Production

2015

New Biotechnologies for Increased Energy Security

View full text Add to dashboard Cite

“…Several detoxification methods have been developed and applied to different hydrolysates. Activated carbon, organic solvent absorbing and extracting inhibitory compounds were proven to be effective physical detoxification methods [11,12]. The chemical detoxification methods include over-liming, reacting with reducing agent and peroxide treatment [13,14].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Compounds in Lignocellulosic Biomass Hydrolysates during Hydrolysate Fermentation Processes

Zha¹,

Muilwijk²,

Coulier³

et al. 2012

J Bioproces Biotechniq

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To compare the composition and performance of various lignocellulosic biomass hydrolysates as fermentation media, 8 hydrolysates were generated from a grass-like and a wood biomass. The hydrolysate preparation methods used were 1) dilute acid, 2) mild alkaline, 3) alkaline/peracetic acid, and 4) concentrated acid. These hydrolysates were fermented at 30°C, pH 5.0 using Saccharomyces cerevisiae CEN.PK113-7D as model strain. The growth in different hydrolysates varied in the aspects of lag-phase, growth rate, glucose consumption rate and ethanol production rate. Subsequently, 11 potential inhibitory compounds as described in literature were selected for further analysis. The concentrations of these compounds were determined in the time-samples of the 8 fermentations, using a novel analytical method, ethyl-chloroformate derivatization-GC-MS. Some of these compounds, e.g. furfural, decreased during the fermentation process, while others, such as formic and benzoic acid, remained almost constant. Inhibitory effect analysis of individual compound revealed that most of these compounds exhibit little effect at the concentrations detected in hydrolysates. Only furfural and benzoic acid clearly affected the growth of the model yeast: furfural elongated the lag-phase, while benzoic acid reduced the growth rate and biomass yield.

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“…It turns out that the presence of inhibitors affects the utilization of xylose in the alcoholic fermentation from Pichia stipitis CBS 5776. The results presented in [46] indicate that the xylose from the detoxified hydrolysate was digested completely after 60 h, and during the fermentation on the hydrolysate without detoxification only 15% of xylose was digested. The results of other studies indicate that the digestibility of xylose depends on its initial concentration in the hydrolysate and on the ratio of glucose to xylose.…”

Section: Influence Of the Pre-treatment On The Bioethanol Yieldmentioning

confidence: 89%

“…The detoxification by extraction with a mixture of trialkylamine, octanol, and kerosene significantly improved the efficiency of obtained bioethanol (21.8 mg/mL), in comparison with the ethanol yield obtained from the hydrolysate without detoxification (3.79 mg/mL) [46]. Despite the poorer results obtained for acid compared to enzymatic hydrolysis, acid hydrolysis may be an attractive method of one-stage hydrolysis prior to alcoholic fermentation.…”

Section: Influence Of the Pre-treatment On The Bioethanol Yieldmentioning

confidence: 93%

Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation

et al. 2018

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This paper concerns the comparison of the efficiency of two-stage hydrolysis processes, i.e., alkaline pre-treatment and acid hydrolysis, as well as alkaline pre-treatment followed by enzymatic hydrolysis, carried out in order to obtain reducing sugars from triticale straw. For each of the analyzed systems, the optimization of the processing conditions was carried out with respect to the glucose yield. For the alkaline pre-treatment, an optimal catalyst concentration was selected for constant values of temperature and pre-treatment time. For enzymatic hydrolysis, optimal process time and concentration of the enzyme preparation were determined. For the acidic hydrolysis, performed with 85% phosphoric acid, the optimum temperature and hydrolysis time were determined. In the hydrolysates obtained after the two-stage treatment, the concentration of reducing sugars was determined using HPLC. The obtained hydrolysates were subjected to ethanol fermentation. The concentrations of fermentation inhibitors are given and their effects on the alcoholic fermentation efficiency are discussed.

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Detoxification of corn stover prehydrolyzate by trialkylamine extraction to improve the ethanol production with Pichia stipitis CBS 5776

Cited by 64 publications

References 25 publications

- Synthetic Biology: A Promising Technology for Biofuel Production

- Synthetic Biology: A Promising Technology for Biofuel Production

Inhibitory Compounds in Lignocellulosic Biomass Hydrolysates during Hydrolysate Fermentation Processes

Comparison and Optimization of Saccharification Conditions of Alkaline Pre-Treated Triticale Straw for Acid and Enzymatic Hydrolysis Followed by Ethanol Fermentation

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